The involvement of Neuregulin-1 in the process of facial nerve injury repair through the utilization of dental pulp stem cells
Abstract Background Facial nerve injury often results in poor prognosis due to the challenging process of nerve regeneration. Neuregulin-1, a human calmodulin, is under investigation in this study for its impact on the reparative capabilities of Dental Pulp Stem Cells (DPSCs) in facial nerve injury....
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-024-03953-z |
| _version_ | 1797273018383204352 |
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| author | Lihong Yao Wanqiu Xu Lixue Liu Xiaohang Xu Hualei Xi Bing Xue Xiaofang Cao Song Lin Guiyan Piao Jian Sun Xiumei Wang |
| author_facet | Lihong Yao Wanqiu Xu Lixue Liu Xiaohang Xu Hualei Xi Bing Xue Xiaofang Cao Song Lin Guiyan Piao Jian Sun Xiumei Wang |
| author_sort | Lihong Yao |
| collection | DOAJ |
| description | Abstract Background Facial nerve injury often results in poor prognosis due to the challenging process of nerve regeneration. Neuregulin-1, a human calmodulin, is under investigation in this study for its impact on the reparative capabilities of Dental Pulp Stem Cells (DPSCs) in facial nerve injury. Methods Lentivirus was used to transfect and construct Neuregulin-1 overexpressed DPSCs. Various techniques assessed the effects of Neuregulin-1: osteogenic induction, lipid induction, Reverse Transcription Polymerase Chain Reaction, Western Blot, Cell Counting Kit-8 assay, wound healing, immunofluorescence, Phalloidin staining, nerve stem action potential, Hematoxylin-eosin staining, transmission electron microscopy, and immunohistochemistry. Results Neuregulin-1 effectively enhanced the proliferation, migration, and cytoskeletal rearrangement of DPSCs, while simultaneously suppressing the expression of Ras homolog gene family member A (RhoA) and Microfilament actin (F-actin). These changes facilitated the neural differentiation of DPSCs. Additionally, in vivo experiments showed that Neuregulin-1 expedited the restoration of action potential in the facial nerve trunk, increased the thickness of the myelin sheath, and stimulated axon regeneration. Conclusion Neuregulin-1 has the capability to facilitate the repair of facial nerve injuries by promoting the regenerative capacity of DPSCs. Thus, Neuregulin-1 is a significant potential gene in the reparative processes of nerve damage. |
| first_indexed | 2024-03-07T14:37:33Z |
| format | Article |
| id | doaj.art-1b4bf438a2e244248f6b44fe7d970f01 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-03-07T14:37:33Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-1b4bf438a2e244248f6b44fe7d970f012024-03-05T20:33:28ZengBMCBMC Oral Health1472-68312024-02-0124111310.1186/s12903-024-03953-zThe involvement of Neuregulin-1 in the process of facial nerve injury repair through the utilization of dental pulp stem cellsLihong Yao0Wanqiu Xu1Lixue Liu2Xiaohang Xu3Hualei Xi4Bing Xue5Xiaofang Cao6Song Lin7Guiyan Piao8Jian Sun9Xiumei Wang10Department of Stomatology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Stomatology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Stomatology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Stomatology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Stomatology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Stomatology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Stomatology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Stomatology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Stomatology, The Second Affiliated Hospital of Harbin Medical UniversityMudanjiang Medical UniversityDepartment of Stomatology, The Second Affiliated Hospital of Harbin Medical UniversityAbstract Background Facial nerve injury often results in poor prognosis due to the challenging process of nerve regeneration. Neuregulin-1, a human calmodulin, is under investigation in this study for its impact on the reparative capabilities of Dental Pulp Stem Cells (DPSCs) in facial nerve injury. Methods Lentivirus was used to transfect and construct Neuregulin-1 overexpressed DPSCs. Various techniques assessed the effects of Neuregulin-1: osteogenic induction, lipid induction, Reverse Transcription Polymerase Chain Reaction, Western Blot, Cell Counting Kit-8 assay, wound healing, immunofluorescence, Phalloidin staining, nerve stem action potential, Hematoxylin-eosin staining, transmission electron microscopy, and immunohistochemistry. Results Neuregulin-1 effectively enhanced the proliferation, migration, and cytoskeletal rearrangement of DPSCs, while simultaneously suppressing the expression of Ras homolog gene family member A (RhoA) and Microfilament actin (F-actin). These changes facilitated the neural differentiation of DPSCs. Additionally, in vivo experiments showed that Neuregulin-1 expedited the restoration of action potential in the facial nerve trunk, increased the thickness of the myelin sheath, and stimulated axon regeneration. Conclusion Neuregulin-1 has the capability to facilitate the repair of facial nerve injuries by promoting the regenerative capacity of DPSCs. Thus, Neuregulin-1 is a significant potential gene in the reparative processes of nerve damage.https://doi.org/10.1186/s12903-024-03953-zDental pulp stem cellsNeuregulin-1ProliferationMigrationNeural differentiationFacial nerve injury |
| spellingShingle | Lihong Yao Wanqiu Xu Lixue Liu Xiaohang Xu Hualei Xi Bing Xue Xiaofang Cao Song Lin Guiyan Piao Jian Sun Xiumei Wang The involvement of Neuregulin-1 in the process of facial nerve injury repair through the utilization of dental pulp stem cells BMC Oral Health Dental pulp stem cells Neuregulin-1 Proliferation Migration Neural differentiation Facial nerve injury |
| title | The involvement of Neuregulin-1 in the process of facial nerve injury repair through the utilization of dental pulp stem cells |
| title_full | The involvement of Neuregulin-1 in the process of facial nerve injury repair through the utilization of dental pulp stem cells |
| title_fullStr | The involvement of Neuregulin-1 in the process of facial nerve injury repair through the utilization of dental pulp stem cells |
| title_full_unstemmed | The involvement of Neuregulin-1 in the process of facial nerve injury repair through the utilization of dental pulp stem cells |
| title_short | The involvement of Neuregulin-1 in the process of facial nerve injury repair through the utilization of dental pulp stem cells |
| title_sort | involvement of neuregulin 1 in the process of facial nerve injury repair through the utilization of dental pulp stem cells |
| topic | Dental pulp stem cells Neuregulin-1 Proliferation Migration Neural differentiation Facial nerve injury |
| url | https://doi.org/10.1186/s12903-024-03953-z |
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