Hippophae salicifolia D.Don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusion
Objective: To investigate the protective effect of Hippophae salicifolia D.Don (H. salicifolia) berries extract against cerebral reperfusion injury induced neurobehavioral and neurochemical changes in a rat model of middle cerebral artery occlusion (MCAO). Methods: Rats were pretreated with alcoholi...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-06-01
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| Series: | Journal of Acute Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221618915300214 |
| _version_ | 1828273505743929344 |
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| author | Santhrani Thakur Pradeepthi Chilikuri Bindu Pulugurtha Lavanya Yaidikar |
| author_facet | Santhrani Thakur Pradeepthi Chilikuri Bindu Pulugurtha Lavanya Yaidikar |
| author_sort | Santhrani Thakur |
| collection | DOAJ |
| description | Objective: To investigate the protective effect of Hippophae salicifolia D.Don (H. salicifolia) berries extract against cerebral reperfusion injury induced neurobehavioral and neurochemical changes in a rat model of middle cerebral artery occlusion (MCAO).
Methods: Rats were pretreated with alcoholic extract of H. salicifolia (250 and 500 mg/kg) for 14 d and focal cerebral ischemia was induced by MCAO. After 60 min of MCAO, reperfused for 24 h, a battery of behavioral tests were assessed the extent of neurological deficits. Infarct volume and brain edema were measured in 2,3,5-triphenyltetrazolium chloride stained brain sections. TNF-α, oxidative stress parameters like reduced glutathione, calcium, glutamate, malondialdehyde and apoptotic parameters like caspase-3, and caspase-9 were estimated in the brain homogenates.
Results: Pretreatment with alcoholic extract of H. salicifolia at doses of 250 and 500 mg/kg significantly improved the neurobehavioral alterations and reduced the infarct volume, edema induced by ischemia reperfusion injury. H. salicifolia significantly prevented ischemia induced increase in malondialdehyde, glutamate, calcium, caspase-3, caspase-9 and TNF-α levels as compared to ischemic animals.
Conclusions: Our results indicate that H. salicifolia mitigated the ischemia reperfusion induced neuronal damage. |
| first_indexed | 2024-04-13T06:21:31Z |
| format | Article |
| id | doaj.art-1b4d1bebb0ea4fd186d938c82b18150b |
| institution | Directory Open Access Journal |
| issn | 2221-6189 |
| language | English |
| last_indexed | 2024-04-13T06:21:31Z |
| publishDate | 2015-06-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Acute Disease |
| spelling | doaj.art-1b4d1bebb0ea4fd186d938c82b18150b2022-12-22T02:58:37ZengWolters Kluwer Medknow PublicationsJournal of Acute Disease2221-61892015-06-014212012810.1016/S2221-6189(15)30021-4Hippophae salicifolia D.Don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusionSanthrani ThakurPradeepthi ChilikuriBindu PulugurthaLavanya YaidikarObjective: To investigate the protective effect of Hippophae salicifolia D.Don (H. salicifolia) berries extract against cerebral reperfusion injury induced neurobehavioral and neurochemical changes in a rat model of middle cerebral artery occlusion (MCAO). Methods: Rats were pretreated with alcoholic extract of H. salicifolia (250 and 500 mg/kg) for 14 d and focal cerebral ischemia was induced by MCAO. After 60 min of MCAO, reperfused for 24 h, a battery of behavioral tests were assessed the extent of neurological deficits. Infarct volume and brain edema were measured in 2,3,5-triphenyltetrazolium chloride stained brain sections. TNF-α, oxidative stress parameters like reduced glutathione, calcium, glutamate, malondialdehyde and apoptotic parameters like caspase-3, and caspase-9 were estimated in the brain homogenates. Results: Pretreatment with alcoholic extract of H. salicifolia at doses of 250 and 500 mg/kg significantly improved the neurobehavioral alterations and reduced the infarct volume, edema induced by ischemia reperfusion injury. H. salicifolia significantly prevented ischemia induced increase in malondialdehyde, glutamate, calcium, caspase-3, caspase-9 and TNF-α levels as compared to ischemic animals. Conclusions: Our results indicate that H. salicifolia mitigated the ischemia reperfusion induced neuronal damage.http://www.sciencedirect.com/science/article/pii/S2221618915300214Hippophae salicifolia D.Don berriesIschemia reperfusion injuryAntioxidantAnti-inflammatoryAnti-apoptotic activities |
| spellingShingle | Santhrani Thakur Pradeepthi Chilikuri Bindu Pulugurtha Lavanya Yaidikar Hippophae salicifolia D.Don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusion Journal of Acute Disease Hippophae salicifolia D.Don berries Ischemia reperfusion injury Antioxidant Anti-inflammatory Anti-apoptotic activities |
| title | Hippophae salicifolia D.Don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusion |
| title_full | Hippophae salicifolia D.Don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusion |
| title_fullStr | Hippophae salicifolia D.Don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusion |
| title_full_unstemmed | Hippophae salicifolia D.Don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusion |
| title_short | Hippophae salicifolia D.Don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusion |
| title_sort | hippophae salicifolia d don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusion |
| topic | Hippophae salicifolia D.Don berries Ischemia reperfusion injury Antioxidant Anti-inflammatory Anti-apoptotic activities |
| url | http://www.sciencedirect.com/science/article/pii/S2221618915300214 |
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