Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic Season
Human respiratory syncytial virus (RSV) is the most common cause of upper and lower respiratory tract infections in infants and young children. It is actively evolving under environmental and herd immunity influences. This work presents, for the first time, sequence variability analysis of RSV G gen...
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MDPI AG
2021-01-01
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| Online Access: | https://www.mdpi.com/1999-4915/13/1/119 |
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| author | Vera Krivitskaya Kseniya Komissarova Maria Pisareva Maria Sverlova Artem Fadeev Ekaterina Petrova Veronika Timonina Anna Sominina Daria Danilenko |
| author_facet | Vera Krivitskaya Kseniya Komissarova Maria Pisareva Maria Sverlova Artem Fadeev Ekaterina Petrova Veronika Timonina Anna Sominina Daria Danilenko |
| author_sort | Vera Krivitskaya |
| collection | DOAJ |
| description | Human respiratory syncytial virus (RSV) is the most common cause of upper and lower respiratory tract infections in infants and young children. It is actively evolving under environmental and herd immunity influences. This work presents, for the first time, sequence variability analysis of RSV G gene and G protein using St. Petersburg (Russia) isolates. Viruses were isolated in a cell culture from the clinical samples of 61 children hospitalized (January–April 2014) with laboratory-confirmed RSV infection. Real-time RT-PCR data showed that 56 isolates (91.8%) belonged to RSV-A and 5 isolates (8.2%) belonged to RSV-B. The G genes were sequenced for 27 RSV-A isolates and all of them belonged to genotype ON1/GA2. Of these RSV-A, 77.8% belonged to the ON1(1.1) genetic sub-cluster, and 14.8% belonged to the ON1(1.2) sub-cluster. The ON1(1.3) sub-cluster constituted a minor group (3.7%). Many single-amino acid substitutions were identified in the G proteins of St. Petersburg isolates, compared with the Canadian ON1/GA2 reference virus (ON67-1210A). Most of the amino acid replacements were found in immunodominant B- and T-cell antigenic determinants of G protein. These may affect the antigenic characteristics of RSV and influence the host antiviral immune response to currently circulating viruses. |
| first_indexed | 2024-03-09T04:31:29Z |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T04:31:29Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1b4d3e6883c241fc819cb88422a4e4b02023-12-03T13:34:32ZengMDPI AGViruses1999-49152021-01-0113111910.3390/v13010119Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic SeasonVera Krivitskaya0Kseniya Komissarova1Maria Pisareva2Maria Sverlova3Artem Fadeev4Ekaterina Petrova5Veronika Timonina6Anna Sominina7Daria Danilenko8Department of Etiology and Epidemiology, Smorodintsev Research Institute of Influenza, 197376 Saint-Petersburg, RussiaDepartment of Etiology and Epidemiology, Smorodintsev Research Institute of Influenza, 197376 Saint-Petersburg, RussiaDepartment of Etiology and Epidemiology, Smorodintsev Research Institute of Influenza, 197376 Saint-Petersburg, RussiaDepartment of Etiology and Epidemiology, Smorodintsev Research Institute of Influenza, 197376 Saint-Petersburg, RussiaDepartment of Etiology and Epidemiology, Smorodintsev Research Institute of Influenza, 197376 Saint-Petersburg, RussiaDepartment of Etiology and Epidemiology, Smorodintsev Research Institute of Influenza, 197376 Saint-Petersburg, RussiaChildren’s City Hospital of St. Olga, 194017 Saint-Petersburg, RussiaDepartment of Etiology and Epidemiology, Smorodintsev Research Institute of Influenza, 197376 Saint-Petersburg, RussiaDepartment of Etiology and Epidemiology, Smorodintsev Research Institute of Influenza, 197376 Saint-Petersburg, RussiaHuman respiratory syncytial virus (RSV) is the most common cause of upper and lower respiratory tract infections in infants and young children. It is actively evolving under environmental and herd immunity influences. This work presents, for the first time, sequence variability analysis of RSV G gene and G protein using St. Petersburg (Russia) isolates. Viruses were isolated in a cell culture from the clinical samples of 61 children hospitalized (January–April 2014) with laboratory-confirmed RSV infection. Real-time RT-PCR data showed that 56 isolates (91.8%) belonged to RSV-A and 5 isolates (8.2%) belonged to RSV-B. The G genes were sequenced for 27 RSV-A isolates and all of them belonged to genotype ON1/GA2. Of these RSV-A, 77.8% belonged to the ON1(1.1) genetic sub-cluster, and 14.8% belonged to the ON1(1.2) sub-cluster. The ON1(1.3) sub-cluster constituted a minor group (3.7%). Many single-amino acid substitutions were identified in the G proteins of St. Petersburg isolates, compared with the Canadian ON1/GA2 reference virus (ON67-1210A). Most of the amino acid replacements were found in immunodominant B- and T-cell antigenic determinants of G protein. These may affect the antigenic characteristics of RSV and influence the host antiviral immune response to currently circulating viruses.https://www.mdpi.com/1999-4915/13/1/119human respiratory syncytial virusphylogenetic analysisON1/GA2 genotypeG protein variability |
| spellingShingle | Vera Krivitskaya Kseniya Komissarova Maria Pisareva Maria Sverlova Artem Fadeev Ekaterina Petrova Veronika Timonina Anna Sominina Daria Danilenko Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic Season Viruses human respiratory syncytial virus phylogenetic analysis ON1/GA2 genotype G protein variability |
| title | Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic Season |
| title_full | Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic Season |
| title_fullStr | Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic Season |
| title_full_unstemmed | Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic Season |
| title_short | Respiratory Syncytial Virus G Protein Sequence Variability among Isolates from St. Petersburg, Russia, during the 2013–2014 Epidemic Season |
| title_sort | respiratory syncytial virus g protein sequence variability among isolates from st petersburg russia during the 2013 2014 epidemic season |
| topic | human respiratory syncytial virus phylogenetic analysis ON1/GA2 genotype G protein variability |
| url | https://www.mdpi.com/1999-4915/13/1/119 |
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