Lack of skin sensitization hazard potential for alpha-glycosyl isoquercitrin (AGIQ) utilizing the Local Lymph Node Assay
Skin sensitization is an important aspect of safety assessment and is a key component in the toxicological evaluation of chemicals. alpha-Glycosyl isoquercitrin (AGIQ), is marketed in Japan as a food additive and is generally recognized as safe (GRAS) by the expert panel of the Flavor and Extract Ma...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Toxicology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750022001366 |
| _version_ | 1811188561127407616 |
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| author | Puneet Vij Douglas A. Donahue Keith P. Burke Shim-mo Hayashi Robert R. Maronpot |
| author_facet | Puneet Vij Douglas A. Donahue Keith P. Burke Shim-mo Hayashi Robert R. Maronpot |
| author_sort | Puneet Vij |
| collection | DOAJ |
| description | Skin sensitization is an important aspect of safety assessment and is a key component in the toxicological evaluation of chemicals. alpha-Glycosyl isoquercitrin (AGIQ), is marketed in Japan as a food additive and is generally recognized as safe (GRAS) by the expert panel of the Flavor and Extract Manufacturers Association (FEMA) in 2005 and the U.S. Food and Drug Administration (FDA) in 2007. The Local Lymph Node Assay (LLNA) was used to assess AGIQ’s potential to cause skin sensitization. Results indicate that no excessive irritation was observed after the irritation screen (ear swelling < 25 % and erythema score < 3) when AGIQ was tested at 5 %, 10 %, and 25 % in N, N-dimethyl formamide [DMF]. Based on lack of irritation, AGIQ was further evaluated at 10 %, 25 %, and 50 % in DMF in the main test resulting in stimulation indices of less than the positive threshold of 1.6 i.e., 1.2, 1.4, and 1.2 respectively. Therefore, AGIQ was not a dermal sensitizer in the LLNA. |
| first_indexed | 2024-04-11T14:20:50Z |
| format | Article |
| id | doaj.art-1b5086f2eaa14955b8101213e898413d |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-04-11T14:20:50Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-1b5086f2eaa14955b8101213e898413d2022-12-22T04:19:03ZengElsevierToxicology Reports2214-75002022-01-01912911296Lack of skin sensitization hazard potential for alpha-glycosyl isoquercitrin (AGIQ) utilizing the Local Lymph Node AssayPuneet Vij0Douglas A. Donahue1Keith P. Burke2Shim-mo Hayashi3Robert R. Maronpot4MB Research Laboratory, 1765 Wentz Road, Spinnerstown, PA 18968, USAIntegrated Laboratory Systems, Inc., PO Box 13501, Research Triangle Park, NC 27709, USAIntegrated Laboratory Systems, Inc., PO Box 13501, Research Triangle Park, NC 27709, USADivision of Food Additives, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, JapanMaronpot Consulting LLC, 1612 Medfield Road, Raleigh, NC 27607, USA; Corresponding author.Skin sensitization is an important aspect of safety assessment and is a key component in the toxicological evaluation of chemicals. alpha-Glycosyl isoquercitrin (AGIQ), is marketed in Japan as a food additive and is generally recognized as safe (GRAS) by the expert panel of the Flavor and Extract Manufacturers Association (FEMA) in 2005 and the U.S. Food and Drug Administration (FDA) in 2007. The Local Lymph Node Assay (LLNA) was used to assess AGIQ’s potential to cause skin sensitization. Results indicate that no excessive irritation was observed after the irritation screen (ear swelling < 25 % and erythema score < 3) when AGIQ was tested at 5 %, 10 %, and 25 % in N, N-dimethyl formamide [DMF]. Based on lack of irritation, AGIQ was further evaluated at 10 %, 25 %, and 50 % in DMF in the main test resulting in stimulation indices of less than the positive threshold of 1.6 i.e., 1.2, 1.4, and 1.2 respectively. Therefore, AGIQ was not a dermal sensitizer in the LLNA.http://www.sciencedirect.com/science/article/pii/S2214750022001366Alpha-glycosyl isoquercitrinAGIQIsoquercitrinSensitizationIrritationLocal Lymph Node Assay |
| spellingShingle | Puneet Vij Douglas A. Donahue Keith P. Burke Shim-mo Hayashi Robert R. Maronpot Lack of skin sensitization hazard potential for alpha-glycosyl isoquercitrin (AGIQ) utilizing the Local Lymph Node Assay Toxicology Reports Alpha-glycosyl isoquercitrin AGIQ Isoquercitrin Sensitization Irritation Local Lymph Node Assay |
| title | Lack of skin sensitization hazard potential for alpha-glycosyl isoquercitrin (AGIQ) utilizing the Local Lymph Node Assay |
| title_full | Lack of skin sensitization hazard potential for alpha-glycosyl isoquercitrin (AGIQ) utilizing the Local Lymph Node Assay |
| title_fullStr | Lack of skin sensitization hazard potential for alpha-glycosyl isoquercitrin (AGIQ) utilizing the Local Lymph Node Assay |
| title_full_unstemmed | Lack of skin sensitization hazard potential for alpha-glycosyl isoquercitrin (AGIQ) utilizing the Local Lymph Node Assay |
| title_short | Lack of skin sensitization hazard potential for alpha-glycosyl isoquercitrin (AGIQ) utilizing the Local Lymph Node Assay |
| title_sort | lack of skin sensitization hazard potential for alpha glycosyl isoquercitrin agiq utilizing the local lymph node assay |
| topic | Alpha-glycosyl isoquercitrin AGIQ Isoquercitrin Sensitization Irritation Local Lymph Node Assay |
| url | http://www.sciencedirect.com/science/article/pii/S2214750022001366 |
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