Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive

Abstract A type of high molecular weight bioactive polymers called exopolysaccharides (EPS) are produced by thermophiles, the extremophilic microbes that thrive in acidic environmental conditions of hot springs with excessively warm temperatures. Over time, EPS became important as natural biotechnol...

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Main Authors: Srijan Banerjee, Gustavo Cabrera-Barjas, Jaime Tapia, João Paulo Fabi, Cedric Delattre, Aparna Banerjee
Format: Article
Language:English
Published: SpringerOpen 2024-02-01
Series:Natural Products and Bioprospecting
Subjects:
Online Access:https://doi.org/10.1007/s13659-024-00436-0
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author Srijan Banerjee
Gustavo Cabrera-Barjas
Jaime Tapia
João Paulo Fabi
Cedric Delattre
Aparna Banerjee
author_facet Srijan Banerjee
Gustavo Cabrera-Barjas
Jaime Tapia
João Paulo Fabi
Cedric Delattre
Aparna Banerjee
author_sort Srijan Banerjee
collection DOAJ
description Abstract A type of high molecular weight bioactive polymers called exopolysaccharides (EPS) are produced by thermophiles, the extremophilic microbes that thrive in acidic environmental conditions of hot springs with excessively warm temperatures. Over time, EPS became important as natural biotechnological additives because of their noncytotoxic, emulsifying, antioxidant, or immunostimulant activities. In this article, we unravelled a new EPS produced by Staphylococcus sp. BSP3 from an acidic (pH 6.03) San Pedro hot spring (38.1 °C) located in the central Andean mountains in Chile. Several physicochemical techniques were performed to characterize the EPS structure including Scanning electron microscopy–energy dispersive X-ray spectroscopy (SEM–EDS), Atomic Force Microscopy (AFM), High-Performance Liquid Chromatography (HPLC), Gel permeation chromatography (GPC), Fourier Transform Infrared Spectroscopy (FTIR), 1D Nuclear Magnetic Resonance (NMR), and Thermogravimetric analysis (TGA). It was confirmed that the amorphous surface of the BSP3 EPS, composed of rough pillar-like nanostructures, is evenly distributed. The main EPS monosaccharide constituents were mannose (72%), glucose (24%) and galactose (4%). Also, it is a medium molecular weight (43.7 kDa) heteropolysaccharide. NMR spectroscopy demonstrated the presence of a [→ 6)-⍺-d-Manp-(1 → 6)-⍺-d-Manp-(1 →] backbone 2-O substituted with 1-⍺-d-Manp. A high thermal stability of EPS (287 °C) was confirmed by TGA analysis. Emulsification, antioxidant, flocculation, water-holding (WHC), and oil-holding (OHC) capacities are   also studied for biotechnological industry applications. The results demonstrated that BSP3 EPS could be used as a biodegradable material for different purposes, like flocculation and natural additives in product formulation. Graphical Abstract
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spelling doaj.art-1b50aeeec9b745bab751826e239004842024-03-05T20:43:14ZengSpringerOpenNatural Products and Bioprospecting2192-21952192-22092024-02-0114111610.1007/s13659-024-00436-0Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additiveSrijan Banerjee0Gustavo Cabrera-Barjas1Jaime Tapia2João Paulo Fabi3Cedric Delattre4Aparna Banerjee5Instituto de Química de Recursos Naturales, Universidad de TalcaUniversidad San Sebastián Campus Las Tres Pascualas, Facultad de Ciencias Para el Cuidado de la SaludInstituto de Química de Recursos Naturales, Universidad de TalcaDepartment of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, University of São PauloUniversité Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut PascalInstituto de Ciencias Aplicadas, Facultad de Ingeniería, Universidad Autónoma de ChileAbstract A type of high molecular weight bioactive polymers called exopolysaccharides (EPS) are produced by thermophiles, the extremophilic microbes that thrive in acidic environmental conditions of hot springs with excessively warm temperatures. Over time, EPS became important as natural biotechnological additives because of their noncytotoxic, emulsifying, antioxidant, or immunostimulant activities. In this article, we unravelled a new EPS produced by Staphylococcus sp. BSP3 from an acidic (pH 6.03) San Pedro hot spring (38.1 °C) located in the central Andean mountains in Chile. Several physicochemical techniques were performed to characterize the EPS structure including Scanning electron microscopy–energy dispersive X-ray spectroscopy (SEM–EDS), Atomic Force Microscopy (AFM), High-Performance Liquid Chromatography (HPLC), Gel permeation chromatography (GPC), Fourier Transform Infrared Spectroscopy (FTIR), 1D Nuclear Magnetic Resonance (NMR), and Thermogravimetric analysis (TGA). It was confirmed that the amorphous surface of the BSP3 EPS, composed of rough pillar-like nanostructures, is evenly distributed. The main EPS monosaccharide constituents were mannose (72%), glucose (24%) and galactose (4%). Also, it is a medium molecular weight (43.7 kDa) heteropolysaccharide. NMR spectroscopy demonstrated the presence of a [→ 6)-⍺-d-Manp-(1 → 6)-⍺-d-Manp-(1 →] backbone 2-O substituted with 1-⍺-d-Manp. A high thermal stability of EPS (287 °C) was confirmed by TGA analysis. Emulsification, antioxidant, flocculation, water-holding (WHC), and oil-holding (OHC) capacities are   also studied for biotechnological industry applications. The results demonstrated that BSP3 EPS could be used as a biodegradable material for different purposes, like flocculation and natural additives in product formulation. Graphical Abstracthttps://doi.org/10.1007/s13659-024-00436-0StaphylococcusHot springExopolysaccharidesStructural characterizationFlocculationAntioxidant activity
spellingShingle Srijan Banerjee
Gustavo Cabrera-Barjas
Jaime Tapia
João Paulo Fabi
Cedric Delattre
Aparna Banerjee
Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive
Natural Products and Bioprospecting
Staphylococcus
Hot spring
Exopolysaccharides
Structural characterization
Flocculation
Antioxidant activity
title Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive
title_full Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive
title_fullStr Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive
title_full_unstemmed Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive
title_short Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive
title_sort characterization of chilean hot spring origin staphylococcus sp bsp3 produced exopolysaccharide as biological additive
topic Staphylococcus
Hot spring
Exopolysaccharides
Structural characterization
Flocculation
Antioxidant activity
url https://doi.org/10.1007/s13659-024-00436-0
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