Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni
A new serological assay dot-dye-immunoassay (dot-DIA) was evaluated for the diagnosis of schistosomiasis mansoni. This method consist of four steps: (a) biding of antigens to a nitrocellulose membrane (NC); (b) blocking of free sites of the NC; (c) incubation in specific primary antibody; (d) detect...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1992-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200003 |
| _version_ | 1797757669708136448 |
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| author | Ana Lúcia Teles Rabello Emmanuel Dias Neto Maria Monica Aguiar Garcia Naftale Katz |
| author_facet | Ana Lúcia Teles Rabello Emmanuel Dias Neto Maria Monica Aguiar Garcia Naftale Katz |
| author_sort | Ana Lúcia Teles Rabello |
| collection | DOAJ |
| description | A new serological assay dot-dye-immunoassay (dot-DIA) was evaluated for the diagnosis of schistosomiasis mansoni. This method consist of four steps: (a) biding of antigens to a nitrocellulose membrane (NC); (b) blocking of free sites of the NC; (c) incubation in specific primary antibody; (d) detection of primary antibody reactivity by color development using second antibody coupled to textile dyes. Sera from 82 individuals, 61 with Schistosoma mansoni eggs in the stool and 21 stool negative were tested by ELISA, dot-ELISA, and dotDIA. A high level of agreement between the methods tested was observed for all sera tested: ELISA x dot-ELISA: 95.1%, ELISA x dot-DIA: 92.7% and dot-ELISA x dot-DIA: 97.6%. In this study, dot-DIA proved to be a feasible, sensitive, rapid and practical test for the diagnosis of shcistosomiasis. |
| first_indexed | 2024-03-12T18:18:56Z |
| format | Article |
| id | doaj.art-1b51798d2001442780466fe2771d8a6c |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:18:56Z |
| publishDate | 1992-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-1b51798d2001442780466fe2771d8a6c2023-08-02T08:59:56ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601992-06-0187218719010.1590/S0074-02761992000200003Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoniAna Lúcia Teles RabelloEmmanuel Dias NetoMaria Monica Aguiar GarciaNaftale KatzA new serological assay dot-dye-immunoassay (dot-DIA) was evaluated for the diagnosis of schistosomiasis mansoni. This method consist of four steps: (a) biding of antigens to a nitrocellulose membrane (NC); (b) blocking of free sites of the NC; (c) incubation in specific primary antibody; (d) detection of primary antibody reactivity by color development using second antibody coupled to textile dyes. Sera from 82 individuals, 61 with Schistosoma mansoni eggs in the stool and 21 stool negative were tested by ELISA, dot-ELISA, and dotDIA. A high level of agreement between the methods tested was observed for all sera tested: ELISA x dot-ELISA: 95.1%, ELISA x dot-DIA: 92.7% and dot-ELISA x dot-DIA: 97.6%. In this study, dot-DIA proved to be a feasible, sensitive, rapid and practical test for the diagnosis of shcistosomiasis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200003Schistosomiasisimmunoassaydot-ELISAdot-DIA |
| spellingShingle | Ana Lúcia Teles Rabello Emmanuel Dias Neto Maria Monica Aguiar Garcia Naftale Katz Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni Memorias do Instituto Oswaldo Cruz Schistosomiasis immunoassay dot-ELISA dot-DIA |
| title | Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni |
| title_full | Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni |
| title_fullStr | Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni |
| title_full_unstemmed | Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni |
| title_short | Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni |
| title_sort | dot dye immunoassay for the diagnosis of schistosomiasis mansoni |
| topic | Schistosomiasis immunoassay dot-ELISA dot-DIA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200003 |
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