Characterization of MDR and ESBL-producing E. coli strains from healthy swine herds of north-eastern India
Fecal samples (225) from apparently healthy pigs were analysed for E. coli isolation. Multidrug-resistant (MDR) isolates (42) were identified from 225 E. coli isolates. Forty isolates exhibited extended-spectrum beta-lactamases (ESBL) phenotype harbouring single to multiple-sized plasmids (1 kb to â...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2019-06-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/91104 |
| _version_ | 1797896659382829056 |
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| author | R K SANJUKTA H SURMANI R K MANDAKINI A A P MILTON S DAS K PURO S GHATAK I SHAKUNTALA A SEN |
| author_facet | R K SANJUKTA H SURMANI R K MANDAKINI A A P MILTON S DAS K PURO S GHATAK I SHAKUNTALA A SEN |
| author_sort | R K SANJUKTA |
| collection | DOAJ |
| description | Fecal samples (225) from apparently healthy pigs were analysed for E. coli isolation. Multidrug-resistant (MDR) isolates (42) were identified from 225 E. coli isolates. Forty isolates exhibited extended-spectrum beta-lactamases (ESBL) phenotype harbouring single to multiple-sized plasmids (1 kb to ≥ 25 kb). Overall blaTEM (85.7%) was the commonest genotype, followed by blaCTX-M (61.9%), blaOXA (17%) and blaSHV was not recovered. Other antimicrobial resistant genes (ARGs) identified were sul2 (100%), strA (95.2%), tetA (92.9%), sul1 (76%), aadA (71.4%), tetB (50%), cat (42.9%) and aac(3)IV (26.2%). Plasmid-mediated quinolone resistance (PMQR) positive strains totalled to 59.5% for qnrB, 19.04% for qnrS but qnrA was not detected. Integron genes were detected either singly or combined: intI1 (76.2%), intI2 (26.2%). Conjugation experiment was successful for 4 ESBL producers. The present study detected higher carriage of ESBL producing E. coli in pigs, and highlights the dominance of resistance and integron genes circulating in MDR E. coli from swine of northeast India, posing a threat of horizontal gene transfer. |
| first_indexed | 2024-04-10T07:46:17Z |
| format | Article |
| id | doaj.art-1b546234702645558c875e1cbd9cfc1f |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:46:17Z |
| publishDate | 2019-06-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-1b546234702645558c875e1cbd9cfc1f2023-02-23T10:34:18ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272019-06-0189610.56093/ijans.v89i6.91104Characterization of MDR and ESBL-producing E. coli strains from healthy swine herds of north-eastern IndiaR K SANJUKTA0H SURMANI1R K MANDAKINI2A A P MILTON3S DAS4K PURO5S GHATAK6I SHAKUNTALA7A SEN8Scientist, ICAR Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 IndiaResearch Scholar, ICAR Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 IndiaResearch Scholar, ICAR Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 IndiaScientist, ICAR Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 IndiaScientist, ICAR Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 IndiaSenior Scientist, ICAR Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 IndiaSenior Scientist, ICAR Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 IndiaPrincipal Scientist, ICAR Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 IndiaPrincipal Scientist, ICAR Research Complex for North-Eastern Hill Region, Umiam, Meghalaya 793 103 IndiaFecal samples (225) from apparently healthy pigs were analysed for E. coli isolation. Multidrug-resistant (MDR) isolates (42) were identified from 225 E. coli isolates. Forty isolates exhibited extended-spectrum beta-lactamases (ESBL) phenotype harbouring single to multiple-sized plasmids (1 kb to ≥ 25 kb). Overall blaTEM (85.7%) was the commonest genotype, followed by blaCTX-M (61.9%), blaOXA (17%) and blaSHV was not recovered. Other antimicrobial resistant genes (ARGs) identified were sul2 (100%), strA (95.2%), tetA (92.9%), sul1 (76%), aadA (71.4%), tetB (50%), cat (42.9%) and aac(3)IV (26.2%). Plasmid-mediated quinolone resistance (PMQR) positive strains totalled to 59.5% for qnrB, 19.04% for qnrS but qnrA was not detected. Integron genes were detected either singly or combined: intI1 (76.2%), intI2 (26.2%). Conjugation experiment was successful for 4 ESBL producers. The present study detected higher carriage of ESBL producing E. coli in pigs, and highlights the dominance of resistance and integron genes circulating in MDR E. coli from swine of northeast India, posing a threat of horizontal gene transfer.https://epubs.icar.org.in/index.php/IJAnS/article/view/91104Antimicrobial resistant genesE. coliESBLsMDRNortheastern IndiaPig |
| spellingShingle | R K SANJUKTA H SURMANI R K MANDAKINI A A P MILTON S DAS K PURO S GHATAK I SHAKUNTALA A SEN Characterization of MDR and ESBL-producing E. coli strains from healthy swine herds of north-eastern India Indian Journal of Animal Sciences Antimicrobial resistant genes E. coli ESBLs MDR Northeastern India Pig |
| title | Characterization of MDR and ESBL-producing E. coli strains from healthy swine herds of north-eastern India |
| title_full | Characterization of MDR and ESBL-producing E. coli strains from healthy swine herds of north-eastern India |
| title_fullStr | Characterization of MDR and ESBL-producing E. coli strains from healthy swine herds of north-eastern India |
| title_full_unstemmed | Characterization of MDR and ESBL-producing E. coli strains from healthy swine herds of north-eastern India |
| title_short | Characterization of MDR and ESBL-producing E. coli strains from healthy swine herds of north-eastern India |
| title_sort | characterization of mdr and esbl producing e coli strains from healthy swine herds of north eastern india |
| topic | Antimicrobial resistant genes E. coli ESBLs MDR Northeastern India Pig |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/91104 |
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