An atlas of cell types in the mouse epididymis and vas deferens
Following testicular spermatogenesis, mammalian sperm continue to mature in a long epithelial tube known as the epididymis, which plays key roles in remodeling sperm protein, lipid, and RNA composition. To understand the roles for the epididymis in reproductive biology, we generated a single-cell at...
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2020-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/55474 |
| _version_ | 1811202685962027008 |
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| author | Vera D Rinaldi Elisa Donnard Kyle Gellatly Morten Rasmussen Alper Kucukural Onur Yukselen Manuel Garber Upasna Sharma Oliver J Rando |
| author_facet | Vera D Rinaldi Elisa Donnard Kyle Gellatly Morten Rasmussen Alper Kucukural Onur Yukselen Manuel Garber Upasna Sharma Oliver J Rando |
| author_sort | Vera D Rinaldi |
| collection | DOAJ |
| description | Following testicular spermatogenesis, mammalian sperm continue to mature in a long epithelial tube known as the epididymis, which plays key roles in remodeling sperm protein, lipid, and RNA composition. To understand the roles for the epididymis in reproductive biology, we generated a single-cell atlas of the murine epididymis and vas deferens. We recovered key epithelial cell types including principal cells, clear cells, and basal cells, along with associated support cells that include fibroblasts, smooth muscle, macrophages and other immune cells. Moreover, our data illuminate extensive regional specialization of principal cell populations across the length of the epididymis. In addition to region-specific specialization of principal cells, we find evidence for functionally specialized subpopulations of stromal cells, and, most notably, two distinct populations of clear cells. Our dataset extends on existing knowledge of epididymal biology, and provides a wealth of information on potential regulatory and signaling factors that bear future investigation. |
| first_indexed | 2024-04-12T02:42:43Z |
| format | Article |
| id | doaj.art-1b5671ed738449c8a1945380abda0587 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:42:43Z |
| publishDate | 2020-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-1b5671ed738449c8a1945380abda05872022-12-22T03:51:16ZengeLife Sciences Publications LtdeLife2050-084X2020-07-01910.7554/eLife.55474An atlas of cell types in the mouse epididymis and vas deferensVera D Rinaldi0https://orcid.org/0000-0002-0051-1754Elisa Donnard1https://orcid.org/0000-0002-8834-8110Kyle Gellatly2Morten Rasmussen3Alper Kucukural4https://orcid.org/0000-0001-9983-394XOnur Yukselen5Manuel Garber6https://orcid.org/0000-0001-8732-1293Upasna Sharma7Oliver J Rando8https://orcid.org/0000-0003-1516-9397Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United StatesDepartment of Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United States; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, United StatesDepartment of Molecular, Cell and Developmental Biology, University of California Santa Cruz, Santa Cruz, United StatesDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, United StatesFollowing testicular spermatogenesis, mammalian sperm continue to mature in a long epithelial tube known as the epididymis, which plays key roles in remodeling sperm protein, lipid, and RNA composition. To understand the roles for the epididymis in reproductive biology, we generated a single-cell atlas of the murine epididymis and vas deferens. We recovered key epithelial cell types including principal cells, clear cells, and basal cells, along with associated support cells that include fibroblasts, smooth muscle, macrophages and other immune cells. Moreover, our data illuminate extensive regional specialization of principal cell populations across the length of the epididymis. In addition to region-specific specialization of principal cells, we find evidence for functionally specialized subpopulations of stromal cells, and, most notably, two distinct populations of clear cells. Our dataset extends on existing knowledge of epididymal biology, and provides a wealth of information on potential regulatory and signaling factors that bear future investigation.https://elifesciences.org/articles/55474reproductionsperm maturationdevelopment |
| spellingShingle | Vera D Rinaldi Elisa Donnard Kyle Gellatly Morten Rasmussen Alper Kucukural Onur Yukselen Manuel Garber Upasna Sharma Oliver J Rando An atlas of cell types in the mouse epididymis and vas deferens eLife reproduction sperm maturation development |
| title | An atlas of cell types in the mouse epididymis and vas deferens |
| title_full | An atlas of cell types in the mouse epididymis and vas deferens |
| title_fullStr | An atlas of cell types in the mouse epididymis and vas deferens |
| title_full_unstemmed | An atlas of cell types in the mouse epididymis and vas deferens |
| title_short | An atlas of cell types in the mouse epididymis and vas deferens |
| title_sort | atlas of cell types in the mouse epididymis and vas deferens |
| topic | reproduction sperm maturation development |
| url | https://elifesciences.org/articles/55474 |
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