Effect of Actisil (Hydroplus™), organic supplements, and pH of the medium on the micropropagation of Vaccinium corymbosum
The effect of commercial Hydroplus™ Actisil, coconut water, coconut milk, and pH of the WPM medium on the micropropagation of V. corymbosum ‘Liberty’ was studied. Three experiments were performed with different concentrations of silicon Hydroplus™ Actisil (Si), coconut water (CW), coconut milk (CM)...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2022-10-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
| Subjects: | |
| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4527 |
| _version_ | 1797394054263078912 |
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| author | Monika Figiel-Kroczyńska Marcelina Krupa-Małkiewicz Ireneusz Ochmian |
| author_facet | Monika Figiel-Kroczyńska Marcelina Krupa-Małkiewicz Ireneusz Ochmian |
| author_sort | Monika Figiel-Kroczyńska |
| collection | DOAJ |
| description |
The effect of commercial Hydroplus™ Actisil, coconut water, coconut milk, and pH of the WPM medium
on the micropropagation of V. corymbosum ‘Liberty’ was studied. Three experiments were performed with
different concentrations of silicon Hydroplus™ Actisil (Si), coconut water (CW), coconut milk (CM), and
different pH as a stress factor. Si was applied at a concentration of 50, 100, 200 and 500 mg dm–3. The highest explant (2.02 cm) with the highest number of new shoots (1.91) and fresh weight (55.16 g) was obtained on WPM medium with the addition of Si in concentration 200 mg dm–3. In experiment 2, similar to 0.1 mg dm–3 zeatin explant growth was achieved when 15% CW was added to the WPM medium (2.13 cm). The use of CM did not have a positive effect on blueberry growth in vitro. The results of experiment 3 indicated that explants of blueberry better developed when pH was lower (5.0) with the highest number of new shoots (2.85) and fresh weight (95.67g). However, there were no significant differences in plant height between pH used. The application of 200 mg dm–3 Actisil benefits the negative effect of higher pH of the WPM medium on micropropagation of blueberry in case of plant height, fresh weight, and biochemical parameters (proline, malondialdehyde – MDA and catalase – CAT activity).
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| first_indexed | 2024-03-09T00:12:52Z |
| format | Article |
| id | doaj.art-1b5754ca90e54594b6cd6ff9825847da |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:12:52Z |
| publishDate | 2022-10-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-1b5754ca90e54594b6cd6ff9825847da2023-12-12T09:39:58ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052022-10-0121510.24326/asphc.2022.5.3Effect of Actisil (Hydroplus™), organic supplements, and pH of the medium on the micropropagation of Vaccinium corymbosumMonika Figiel-Kroczyńska0Marcelina Krupa-Małkiewicz1Ireneusz Ochmian2https://orcid.org/0000-0003-4378-4004Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology Szczecin, Szczecin 71-434, PolandDepartment of Horticulture, West Pomeranian University of Technology Szczecin, Słowackiego 17 Street, 71-434 Szczecin, Poland The effect of commercial Hydroplus™ Actisil, coconut water, coconut milk, and pH of the WPM medium on the micropropagation of V. corymbosum ‘Liberty’ was studied. Three experiments were performed with different concentrations of silicon Hydroplus™ Actisil (Si), coconut water (CW), coconut milk (CM), and different pH as a stress factor. Si was applied at a concentration of 50, 100, 200 and 500 mg dm–3. The highest explant (2.02 cm) with the highest number of new shoots (1.91) and fresh weight (55.16 g) was obtained on WPM medium with the addition of Si in concentration 200 mg dm–3. In experiment 2, similar to 0.1 mg dm–3 zeatin explant growth was achieved when 15% CW was added to the WPM medium (2.13 cm). The use of CM did not have a positive effect on blueberry growth in vitro. The results of experiment 3 indicated that explants of blueberry better developed when pH was lower (5.0) with the highest number of new shoots (2.85) and fresh weight (95.67g). However, there were no significant differences in plant height between pH used. The application of 200 mg dm–3 Actisil benefits the negative effect of higher pH of the WPM medium on micropropagation of blueberry in case of plant height, fresh weight, and biochemical parameters (proline, malondialdehyde – MDA and catalase – CAT activity). https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4527blueberryin vitrozeatinsiliconWoody Plant Mediumcoconut water and milk |
| spellingShingle | Monika Figiel-Kroczyńska Marcelina Krupa-Małkiewicz Ireneusz Ochmian Effect of Actisil (Hydroplus™), organic supplements, and pH of the medium on the micropropagation of Vaccinium corymbosum Acta Scientiarum Polonorum: Hortorum Cultus blueberry in vitro zeatin silicon Woody Plant Medium coconut water and milk |
| title | Effect of Actisil (Hydroplus™), organic supplements, and pH of the medium on the micropropagation of Vaccinium corymbosum |
| title_full | Effect of Actisil (Hydroplus™), organic supplements, and pH of the medium on the micropropagation of Vaccinium corymbosum |
| title_fullStr | Effect of Actisil (Hydroplus™), organic supplements, and pH of the medium on the micropropagation of Vaccinium corymbosum |
| title_full_unstemmed | Effect of Actisil (Hydroplus™), organic supplements, and pH of the medium on the micropropagation of Vaccinium corymbosum |
| title_short | Effect of Actisil (Hydroplus™), organic supplements, and pH of the medium on the micropropagation of Vaccinium corymbosum |
| title_sort | effect of actisil hydroplus™ organic supplements and ph of the medium on the micropropagation of vaccinium corymbosum |
| topic | blueberry in vitro zeatin silicon Woody Plant Medium coconut water and milk |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4527 |
| work_keys_str_mv | AT monikafigielkroczynska effectofactisilhydroplusorganicsupplementsandphofthemediumonthemicropropagationofvacciniumcorymbosum AT marcelinakrupamałkiewicz effectofactisilhydroplusorganicsupplementsandphofthemediumonthemicropropagationofvacciniumcorymbosum AT ireneuszochmian effectofactisilhydroplusorganicsupplementsandphofthemediumonthemicropropagationofvacciniumcorymbosum |