Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170
The protective effects of two probiotic mixtures was studied using the fingerprints of the bacterial community of Litopenaeus vannamei juveniles exposed to probiotics and challenged with Vibrio parahaemolyticus CAIM 170. Fingerprints were constructed using 16S rRNA gene and the PCR-SSCP (Single stra...
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| Format: | Article |
| Language: | English |
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Escuela de Ciencias del Mar, Facultad de Recursos Naturales, Pontificia Universidad Católica de Valparaíso
2015-09-01
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| Series: | Latin American Journal of Aquatic Research |
| Subjects: | |
| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400015&lng=en&tlng=en |
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| author | Irasema E Luis-Villaseñor Domenico Voltolina Bruno Gomez-Gil Felipe Ascencio Ángel I Campa-Córdova Juan M Audelo-Naranjo Olga O Zamudio-Armenta |
| author_facet | Irasema E Luis-Villaseñor Domenico Voltolina Bruno Gomez-Gil Felipe Ascencio Ángel I Campa-Córdova Juan M Audelo-Naranjo Olga O Zamudio-Armenta |
| author_sort | Irasema E Luis-Villaseñor |
| collection | DOAJ |
| description | The protective effects of two probiotic mixtures was studied using the fingerprints of the bacterial community of Litopenaeus vannamei juveniles exposed to probiotics and challenged with Vibrio parahaemolyticus CAIM 170. Fingerprints were constructed using 16S rRNA gene and the PCR-SSCP (Single strand conformation polymorphism) technique, and the probiotics used were an experimental Bacillus mixture (Bacillus tequilensis YC5-2 + B. endophyticus C2-2 and YC3-B) and the commercial probiotic Alibio. The DNA for PCR-SSCP analyses was extracted directly from the guts of shrimps treated for 20 days with the probiotics and injected with 2.5*10(5) CFU g-1 of V. parahaemolyticus one week after suspension of the probiotic treatment. Untreated shrimps served as positive (injected with V. parahaemolyticus) and negative (not injected) controls Analysis of the bacterial community carried out after inoculation and 12 and 48 h later confirmed that V. parahaemolyticus was present in shrimps of the positive control , but not in the negative control or treated with the probiotic mixtures. A significant difference in the diversity of the bacterial community was observed between times after infection. The band patterns in 0-12 h were clustered into a different group from that determined after 48 h, and suggested that during bacterial infection the guts of whiteleg shrimp were dominated by gamma proteobacteria represented by Vibrio sp. and Photobacterium sp. Our results indicate that the experimental and the commercial mixtures are suitable to modulate the bacterial community of L. vannamei and could be used as a probiotic to control vibriosis in juvenile shrimp. |
| first_indexed | 2024-12-16T10:04:12Z |
| format | Article |
| id | doaj.art-1b5a42a30e7047dab9f51ec6a22c8fc5 |
| institution | Directory Open Access Journal |
| issn | 0718-560X |
| language | English |
| last_indexed | 2024-12-16T10:04:12Z |
| publishDate | 2015-09-01 |
| publisher | Escuela de Ciencias del Mar, Facultad de Recursos Naturales, Pontificia Universidad Católica de Valparaíso |
| record_format | Article |
| series | Latin American Journal of Aquatic Research |
| spelling | doaj.art-1b5a42a30e7047dab9f51ec6a22c8fc52022-12-21T22:35:43ZengEscuela de Ciencias del Mar, Facultad de Recursos Naturales, Pontificia Universidad Católica de ValparaísoLatin American Journal of Aquatic Research0718-560X2015-09-0143476677510.3856/vol43-issue4-fulltext-15S0718-560X2015000400015Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170Irasema E Luis-Villaseñor0Domenico Voltolina1Bruno Gomez-Gil2Felipe Ascencio3Ángel I Campa-Córdova4Juan M Audelo-Naranjo5Olga O Zamudio-Armenta6Universidad Autónoma de SinaloaCentro de Investigaciones Biológicas del NoroesteCentro de Investigación en Alimentación y DesarrolloCentro de Investigaciones Biológicas del NoroesteCentro de Investigaciones Biológicas del NoroesteUniversidad Autónoma de SinaloaUniversidad Autónoma de SinaloaThe protective effects of two probiotic mixtures was studied using the fingerprints of the bacterial community of Litopenaeus vannamei juveniles exposed to probiotics and challenged with Vibrio parahaemolyticus CAIM 170. Fingerprints were constructed using 16S rRNA gene and the PCR-SSCP (Single strand conformation polymorphism) technique, and the probiotics used were an experimental Bacillus mixture (Bacillus tequilensis YC5-2 + B. endophyticus C2-2 and YC3-B) and the commercial probiotic Alibio. The DNA for PCR-SSCP analyses was extracted directly from the guts of shrimps treated for 20 days with the probiotics and injected with 2.5*10(5) CFU g-1 of V. parahaemolyticus one week after suspension of the probiotic treatment. Untreated shrimps served as positive (injected with V. parahaemolyticus) and negative (not injected) controls Analysis of the bacterial community carried out after inoculation and 12 and 48 h later confirmed that V. parahaemolyticus was present in shrimps of the positive control , but not in the negative control or treated with the probiotic mixtures. A significant difference in the diversity of the bacterial community was observed between times after infection. The band patterns in 0-12 h were clustered into a different group from that determined after 48 h, and suggested that during bacterial infection the guts of whiteleg shrimp were dominated by gamma proteobacteria represented by Vibrio sp. and Photobacterium sp. Our results indicate that the experimental and the commercial mixtures are suitable to modulate the bacterial community of L. vannamei and could be used as a probiotic to control vibriosis in juvenile shrimp.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400015&lng=en&tlng=enLitopenaeus vannameimezcla de BacillusVibrio parahaemolyticuscomunidad bacterianaacuicultura |
| spellingShingle | Irasema E Luis-Villaseñor Domenico Voltolina Bruno Gomez-Gil Felipe Ascencio Ángel I Campa-Córdova Juan M Audelo-Naranjo Olga O Zamudio-Armenta Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170 Latin American Journal of Aquatic Research Litopenaeus vannamei mezcla de Bacillus Vibrio parahaemolyticus comunidad bacteriana acuicultura |
| title | Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170 |
| title_full | Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170 |
| title_fullStr | Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170 |
| title_full_unstemmed | Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170 |
| title_short | Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170 |
| title_sort | probiotic modulation of the gut bacterial community of juvenile litopenaeus vannamei challenged with vibrio parahaemolyticus caim 170 |
| topic | Litopenaeus vannamei mezcla de Bacillus Vibrio parahaemolyticus comunidad bacteriana acuicultura |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400015&lng=en&tlng=en |
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