Serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancer
Endometriosis and endometrial cancer are closely related to oxidative stress. However, the direct relationship between copper and zinc levels and oxidative stress in the extracellular and intracellular space remains unclear. The presented study is focused on the determination of serum Zn and Cu leve...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13738 |
| _version_ | 1797367587618684928 |
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| author | Miroslava Rabajdová Ivana Špaková Lukáš Smolko Michaela Abrahamovská Barbora Baranovičová Anna Birková Janka Vašková Mária Mareková |
| author_facet | Miroslava Rabajdová Ivana Špaková Lukáš Smolko Michaela Abrahamovská Barbora Baranovičová Anna Birková Janka Vašková Mária Mareková |
| author_sort | Miroslava Rabajdová |
| collection | DOAJ |
| description | Endometriosis and endometrial cancer are closely related to oxidative stress. However, the direct relationship between copper and zinc levels and oxidative stress in the extracellular and intracellular space remains unclear. The presented study is focused on the determination of serum Zn and Cu levels, glutathione concentration and enzyme activity in three groups: patients diagnosed with endometrial cancer (EC), patients diagnosed with endometriosis (EM), and a healthy control group. Spectrophotometric determination of trace elements revealed that levels of zinc and copper were lower in blood plasma of patients with endometriosis as compared with the other groups; however, there were no significant differences in the Cu/Zn ratio. Furthermore, significantly increased blood serum glutathione levels were detected in both EM and EC groups compared with the control group. While the activity of superoxide dismutase (SOD) was similar across the studied groups, we observed differences in the activity of other enzymes associated with oxidative stress, including glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S‐transferase (GST), between the control group and the EM and EC patients. Additionally, analysis of gene expression based on free circulating mRNA indicated significant differences in the expression of SOD isoenzymes between the patient groups and the control group; expression of GPx isoenzymes was also altered. Obtained results may have potential application in diagnostics as well as monitoring of endometriosis and endometrial cancer. |
| first_indexed | 2024-03-08T17:20:29Z |
| format | Article |
| id | doaj.art-1b5ef68142c644ddb499b3345ba05050 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-08T17:20:29Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-1b5ef68142c644ddb499b3345ba050502024-01-03T05:28:44ZengWileyFEBS Open Bio2211-54632024-01-0114114815710.1002/2211-5463.13738Serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancerMiroslava Rabajdová0Ivana Špaková1Lukáš Smolko2Michaela Abrahamovská3Barbora Baranovičová4Anna Birková5Janka Vašková6Mária Mareková7Department of Medical and Clinical Biochemistry, Faculty of Medicine P. J. Šafárik University in Košice SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine P. J. Šafárik University in Košice SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine P. J. Šafárik University in Košice SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine P. J. Šafárik University in Košice SlovakiaDepartment of Gynaecology and Obstetrics, Faculty of Medicine P. J. Šafárik University in Košice SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine P. J. Šafárik University in Košice SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine P. J. Šafárik University in Košice SlovakiaDepartment of Medical and Clinical Biochemistry, Faculty of Medicine P. J. Šafárik University in Košice SlovakiaEndometriosis and endometrial cancer are closely related to oxidative stress. However, the direct relationship between copper and zinc levels and oxidative stress in the extracellular and intracellular space remains unclear. The presented study is focused on the determination of serum Zn and Cu levels, glutathione concentration and enzyme activity in three groups: patients diagnosed with endometrial cancer (EC), patients diagnosed with endometriosis (EM), and a healthy control group. Spectrophotometric determination of trace elements revealed that levels of zinc and copper were lower in blood plasma of patients with endometriosis as compared with the other groups; however, there were no significant differences in the Cu/Zn ratio. Furthermore, significantly increased blood serum glutathione levels were detected in both EM and EC groups compared with the control group. While the activity of superoxide dismutase (SOD) was similar across the studied groups, we observed differences in the activity of other enzymes associated with oxidative stress, including glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S‐transferase (GST), between the control group and the EM and EC patients. Additionally, analysis of gene expression based on free circulating mRNA indicated significant differences in the expression of SOD isoenzymes between the patient groups and the control group; expression of GPx isoenzymes was also altered. Obtained results may have potential application in diagnostics as well as monitoring of endometriosis and endometrial cancer.https://doi.org/10.1002/2211-5463.13738coppergene expressionglutathionesuperoxide dismutasezinc |
| spellingShingle | Miroslava Rabajdová Ivana Špaková Lukáš Smolko Michaela Abrahamovská Barbora Baranovičová Anna Birková Janka Vašková Mária Mareková Serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancer FEBS Open Bio copper gene expression glutathione superoxide dismutase zinc |
| title | Serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancer |
| title_full | Serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancer |
| title_fullStr | Serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancer |
| title_full_unstemmed | Serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancer |
| title_short | Serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancer |
| title_sort | serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancer |
| topic | copper gene expression glutathione superoxide dismutase zinc |
| url | https://doi.org/10.1002/2211-5463.13738 |
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