Expansion of KPC-producing Enterobacterales in four large hospitals in Hanoi, Vietnam
ABSTRACT: Objectives: : The incidence of carbapenem resistance among nosocomial Gram-negative bacteria in Vietnam is high and increasing, including among Enterobacterales. In this study, we assessed the presence of one of the main carbapenemase genes, blaKPC, among carbapenem-resistant Enterobacter...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716521002137 |
| _version_ | 1798026685325508608 |
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| author | Tran Dieu Linh Nguyen Hoai Thu Keigo Shibayama Masato Suzuki LayMint Yoshida Pham Duy Thai Dang Duc Anh Tran Nhu Duong Hong Son Trinh Vu Phuong Thom Luu Thi Vu Nga Nguyen Thi Kim Phuong Bui Thanh Thuyet Timothy R. Walsh Le Viet Thanh Anne-Laure Bañuls H. Rogier van Doorn Tran Van Anh Tran Huy Hoang |
| author_facet | Tran Dieu Linh Nguyen Hoai Thu Keigo Shibayama Masato Suzuki LayMint Yoshida Pham Duy Thai Dang Duc Anh Tran Nhu Duong Hong Son Trinh Vu Phuong Thom Luu Thi Vu Nga Nguyen Thi Kim Phuong Bui Thanh Thuyet Timothy R. Walsh Le Viet Thanh Anne-Laure Bañuls H. Rogier van Doorn Tran Van Anh Tran Huy Hoang |
| author_sort | Tran Dieu Linh |
| collection | DOAJ |
| description | ABSTRACT: Objectives: : The incidence of carbapenem resistance among nosocomial Gram-negative bacteria in Vietnam is high and increasing, including among Enterobacterales. In this study, we assessed the presence of one of the main carbapenemase genes, blaKPC, among carbapenem-resistant Enterobacterales (CRE) from four large hospitals in Hanoi, Vietnam, between 2010 and 2015, and described their key molecular characteristics. Methods: : KPC-producing Enterobacterales were detected using conventional PCR and were further analysed using S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), Southern blotting and whole-genome sequencing (WGS) for sequence typing and genetic characterisation. Results: : blaKPC genes were detected in 122 (20.4%) of 599 CRE isolates. blaKPC-carrying plasmids were diverse in size. Klebsiella pneumoniae harbouring blaKPC genes belonged to ST15 and ST11, whereas KPC-producing Escherichia coli showed more diverse sequence types including ST3580, ST448, ST709 and ST405. Genotypic relationships supported the hypothesis of circulation of a population of ‘resident’ resistant bacteria in one hospital through the years and of transmission among these hospitals via patient transfer. WGS results revealed co-carriage of several other antimicrobial resistance genes and three different genetic contexts of blaKPC-2. Among these, the combination of ISEcp1–blaCTX-M and ISKpn27–blaKPC–ΔISKpn6 on the same plasmid is reported for the first time. Conclusion: : We describe the dissemination of blaKPC-expressing Enterobacterales in four large hospitals in Hanoi, Vietnam, since 2010, which may have started earlier, along with their resistance patterns, sequence types, genotypic relationship, plasmid sizes and genetic context, thereby contributing to the overall picture of the antimicrobial resistance situation in Enterobacterales in Vietnam. |
| first_indexed | 2024-04-11T18:40:24Z |
| format | Article |
| id | doaj.art-1b5f493c8d264ccb9d06a21070ad7de4 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-04-11T18:40:24Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-1b5f493c8d264ccb9d06a21070ad7de42022-12-22T04:09:02ZengElsevierJournal of Global Antimicrobial Resistance2213-71652021-12-0127200211Expansion of KPC-producing Enterobacterales in four large hospitals in Hanoi, VietnamTran Dieu Linh0Nguyen Hoai Thu1Keigo Shibayama2Masato Suzuki3LayMint Yoshida4Pham Duy Thai5Dang Duc Anh6Tran Nhu Duong7Hong Son Trinh8Vu Phuong Thom9Luu Thi Vu Nga10Nguyen Thi Kim Phuong11Bui Thanh Thuyet12Timothy R. Walsh13Le Viet Thanh14Anne-Laure Bañuls15H. Rogier van Doorn16Tran Van Anh17Tran Huy Hoang18National Institute of Hygiene and Epidemiology, Hanoi, VietnamNational Institute of Hygiene and Epidemiology, Hanoi, VietnamNational Institute of Infectious Diseases, Tokyo, JapanNational Institute of Infectious Diseases, Tokyo, JapanInstitute of Tropical Diseases, Nagasaki University, Nagasaki, JapanNational Institute of Hygiene and Epidemiology, Hanoi, VietnamNational Institute of Hygiene and Epidemiology, Hanoi, VietnamNational Institute of Hygiene and Epidemiology, Hanoi, VietnamViet Duc Hospital, Hanoi, VietnamSaint Paul Hospital, Hanoi, VietnamThanh Nhan Hospital, Hanoi, Vietnam108 Military Central Hospital, Hanoi, Vietnam108 Military Central Hospital, Hanoi, VietnamDepartment of Medical Microbiology and Infectious Disease, Cardiff University, Cardiff, UKOxford University Clinical Research Unit, Hanoi, Vietnam; Quadram Institute Bioscience, Norwich Research Park, Norwich, Norfolk, UKMIVEGEC (IRD-CNRS-Université de Montpellier), Centre IRD, Montpellier, FranceOxford University Clinical Research Unit, Hanoi, Vietnam; Centre for Tropical Medicine and Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UKHanoi Medical University, Hanoi, VietnamNational Institute of Hygiene and Epidemiology, Hanoi, Vietnam; Hanoi Medical University, Hanoi, Vietnam; Corresponding author. National Institute of Hygiene and Epidemiology, 1 Yersin Street, Hanoi 100 000, Vietnam. Tel.: +84 8 3426 9368.ABSTRACT: Objectives: : The incidence of carbapenem resistance among nosocomial Gram-negative bacteria in Vietnam is high and increasing, including among Enterobacterales. In this study, we assessed the presence of one of the main carbapenemase genes, blaKPC, among carbapenem-resistant Enterobacterales (CRE) from four large hospitals in Hanoi, Vietnam, between 2010 and 2015, and described their key molecular characteristics. Methods: : KPC-producing Enterobacterales were detected using conventional PCR and were further analysed using S1 nuclease pulsed-field gel electrophoresis (S1-PFGE), Southern blotting and whole-genome sequencing (WGS) for sequence typing and genetic characterisation. Results: : blaKPC genes were detected in 122 (20.4%) of 599 CRE isolates. blaKPC-carrying plasmids were diverse in size. Klebsiella pneumoniae harbouring blaKPC genes belonged to ST15 and ST11, whereas KPC-producing Escherichia coli showed more diverse sequence types including ST3580, ST448, ST709 and ST405. Genotypic relationships supported the hypothesis of circulation of a population of ‘resident’ resistant bacteria in one hospital through the years and of transmission among these hospitals via patient transfer. WGS results revealed co-carriage of several other antimicrobial resistance genes and three different genetic contexts of blaKPC-2. Among these, the combination of ISEcp1–blaCTX-M and ISKpn27–blaKPC–ΔISKpn6 on the same plasmid is reported for the first time. Conclusion: : We describe the dissemination of blaKPC-expressing Enterobacterales in four large hospitals in Hanoi, Vietnam, since 2010, which may have started earlier, along with their resistance patterns, sequence types, genotypic relationship, plasmid sizes and genetic context, thereby contributing to the overall picture of the antimicrobial resistance situation in Enterobacterales in Vietnam.http://www.sciencedirect.com/science/article/pii/S2213716521002137Carbapenem resistanceKPCEnterobacteralesVietnam |
| spellingShingle | Tran Dieu Linh Nguyen Hoai Thu Keigo Shibayama Masato Suzuki LayMint Yoshida Pham Duy Thai Dang Duc Anh Tran Nhu Duong Hong Son Trinh Vu Phuong Thom Luu Thi Vu Nga Nguyen Thi Kim Phuong Bui Thanh Thuyet Timothy R. Walsh Le Viet Thanh Anne-Laure Bañuls H. Rogier van Doorn Tran Van Anh Tran Huy Hoang Expansion of KPC-producing Enterobacterales in four large hospitals in Hanoi, Vietnam Journal of Global Antimicrobial Resistance Carbapenem resistance KPC Enterobacterales Vietnam |
| title | Expansion of KPC-producing Enterobacterales in four large hospitals in Hanoi, Vietnam |
| title_full | Expansion of KPC-producing Enterobacterales in four large hospitals in Hanoi, Vietnam |
| title_fullStr | Expansion of KPC-producing Enterobacterales in four large hospitals in Hanoi, Vietnam |
| title_full_unstemmed | Expansion of KPC-producing Enterobacterales in four large hospitals in Hanoi, Vietnam |
| title_short | Expansion of KPC-producing Enterobacterales in four large hospitals in Hanoi, Vietnam |
| title_sort | expansion of kpc producing enterobacterales in four large hospitals in hanoi vietnam |
| topic | Carbapenem resistance KPC Enterobacterales Vietnam |
| url | http://www.sciencedirect.com/science/article/pii/S2213716521002137 |
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