POMC expression of the urothelium of the urinary bladder of mice submitted to pelvic radiation
Objective: Patients who have had pelvic radiotherapy as part of their cancer therapy may develop subsequent urinary bladder injury. The acute changes that the urothelium undergo after radiation are known, but the healing mechanism of the urothelium of the urinary bladder after pelvic radiotherapy is...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2016-04-01
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| Series: | European Journal of Inflammation |
| Online Access: | https://doi.org/10.1177/1721727X15624698 |
| _version_ | 1818317485535920128 |
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| author | M Kemal Ozbilgin Caner Aktas Merve Temel Tuna Önal Elgin Türköz Uluer H Seda Vatansever Cengiz Kurtman |
| author_facet | M Kemal Ozbilgin Caner Aktas Merve Temel Tuna Önal Elgin Türköz Uluer H Seda Vatansever Cengiz Kurtman |
| author_sort | M Kemal Ozbilgin |
| collection | DOAJ |
| description | Objective: Patients who have had pelvic radiotherapy as part of their cancer therapy may develop subsequent urinary bladder injury. The acute changes that the urothelium undergo after radiation are known, but the healing mechanism of the urothelium of the urinary bladder after pelvic radiotherapy is not clearly understood. Proopiomelanocortin (POMC) peptides, which have immunomodulatory effects, are produced locally in sites outside of the central nervous system. This study aims to determine the role of POMC expression in the urothelium during radiation injury. Methods: Twenty-four male Swiss Albino mice were divided into four groups. A single-fractioned 10 Gy of ionizing radiation was applied to the pelvic zone of all mice with Cobalt-60 radiotherapy. The first group 1, which consisted intact animal and not irradiated was the control group, and the second, third, and fourth groups were euthanized after 24 h (Group 2), 48 h (Group 3), and 7 days (Group 4) after irradiation. All bladders were prepared for histochemical analysis using hematoxylin eosin (H&E) and immunohistochemical analysis using anti-POMC antibody. Results: No morphological differences were seen in all the group samples stained with H&E. POMC expression of the urothelium of bladder tissue samples shows different staining levels. Group 1 (96.7 ± 7.68), Group 2 (88.3 ± 8.04), and Group 3 (85.10 ± 10.9) were very weakly stained, but the POMC immunoreactivity of Group 4 (113.0 ± 12.8) was observed to be strong. Conclusion: Expression of POMC from urothelium seems to prevent bladder damage from radiation supplying differentiation and restoration of the urothelium. |
| first_indexed | 2024-12-13T09:38:05Z |
| format | Article |
| id | doaj.art-1b5f7ab6a27d4d3ea7b97cbbfbc37479 |
| institution | Directory Open Access Journal |
| issn | 1721-727X |
| language | English |
| last_indexed | 2024-12-13T09:38:05Z |
| publishDate | 2016-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | European Journal of Inflammation |
| spelling | doaj.art-1b5f7ab6a27d4d3ea7b97cbbfbc374792022-12-21T23:52:17ZengSAGE PublishingEuropean Journal of Inflammation1721-727X2016-04-011410.1177/1721727X15624698POMC expression of the urothelium of the urinary bladder of mice submitted to pelvic radiationM Kemal Ozbilgin0Caner Aktas1Merve Temel2Tuna Önal3Elgin Türköz Uluer4H Seda Vatansever5Cengiz Kurtman6Celal Bayar University School of Medicine, Department of Histology and Embryology, Manisa, TurkeyDepartment of Radiation Oncology, Diskapi Training and Research Hospital, Ankara, TurkeyCelal Bayar University School of Medicine, Department of Histology and Embryology, Manisa, TurkeyCelal Bayar University School of Medicine, Department of Histology and Embryology, Manisa, TurkeyCelal Bayar University School of Medicine, Department of Histology and Embryology, Manisa, TurkeyNear East University, Experimental Health Research Center of Health Sciences, Nicosia, North CyprusDepartment of Radiation Oncology, Ankara University School of Medicine, Ankara, TurkeyObjective: Patients who have had pelvic radiotherapy as part of their cancer therapy may develop subsequent urinary bladder injury. The acute changes that the urothelium undergo after radiation are known, but the healing mechanism of the urothelium of the urinary bladder after pelvic radiotherapy is not clearly understood. Proopiomelanocortin (POMC) peptides, which have immunomodulatory effects, are produced locally in sites outside of the central nervous system. This study aims to determine the role of POMC expression in the urothelium during radiation injury. Methods: Twenty-four male Swiss Albino mice were divided into four groups. A single-fractioned 10 Gy of ionizing radiation was applied to the pelvic zone of all mice with Cobalt-60 radiotherapy. The first group 1, which consisted intact animal and not irradiated was the control group, and the second, third, and fourth groups were euthanized after 24 h (Group 2), 48 h (Group 3), and 7 days (Group 4) after irradiation. All bladders were prepared for histochemical analysis using hematoxylin eosin (H&E) and immunohistochemical analysis using anti-POMC antibody. Results: No morphological differences were seen in all the group samples stained with H&E. POMC expression of the urothelium of bladder tissue samples shows different staining levels. Group 1 (96.7 ± 7.68), Group 2 (88.3 ± 8.04), and Group 3 (85.10 ± 10.9) were very weakly stained, but the POMC immunoreactivity of Group 4 (113.0 ± 12.8) was observed to be strong. Conclusion: Expression of POMC from urothelium seems to prevent bladder damage from radiation supplying differentiation and restoration of the urothelium.https://doi.org/10.1177/1721727X15624698 |
| spellingShingle | M Kemal Ozbilgin Caner Aktas Merve Temel Tuna Önal Elgin Türköz Uluer H Seda Vatansever Cengiz Kurtman POMC expression of the urothelium of the urinary bladder of mice submitted to pelvic radiation European Journal of Inflammation |
| title | POMC expression of the urothelium of the urinary bladder of mice submitted to pelvic radiation |
| title_full | POMC expression of the urothelium of the urinary bladder of mice submitted to pelvic radiation |
| title_fullStr | POMC expression of the urothelium of the urinary bladder of mice submitted to pelvic radiation |
| title_full_unstemmed | POMC expression of the urothelium of the urinary bladder of mice submitted to pelvic radiation |
| title_short | POMC expression of the urothelium of the urinary bladder of mice submitted to pelvic radiation |
| title_sort | pomc expression of the urothelium of the urinary bladder of mice submitted to pelvic radiation |
| url | https://doi.org/10.1177/1721727X15624698 |
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