| Summary: | The aim of this study was to investigate the antioxidant and free-radical scavenging activity of extract and fractions from various parts of <em>Elsholtzia ciliata</em>. The inflorescences, leaves, stems and roots of <em>E. ciliata</em> were extracted separately and two phenolic component enrichment methods: ethyl acetate-water liquid-liquid extraction and macroporous resin adsorption-desorption, were adopted in this study. The antioxidant activities of water extracts and fractions of <em>E. ciliata</em> were examined using different assay model systems <em>in vitro</em>. The fraction root E (purified by HPD300 macroporous resin) exhibited the highest total phenolics content (497.2 ± 24.9 mg GAE/g), accompanied with the highest antioxidant activity against various antioxidant systems <em>in vitro</em> compared to other fractions. On the basis of the results obtained, <em>E. ciliata</em> extracts can be used potentially as a ready accessible and valuable bioactive source of natural antioxidants.
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