A rapid and efficient DNA extraction method from high oily content seeds:
Ricinus communis seeds harbor high oil and polyphenolics contents that hinder DNA extraction. Here, a rapid and efficient protocol for isolating total DNA from R. communis seeds was developed. The current method implies the use of repeated cycles of freeze/heat shock for the seed tissue to lyse the...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2023-12-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/16094 |
| _version_ | 1797390860401246208 |
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| author | Elham R. S. SOLIMAN |
| author_facet | Elham R. S. SOLIMAN |
| author_sort | Elham R. S. SOLIMAN |
| collection | DOAJ |
| description | Ricinus communis seeds harbor high oil and polyphenolics contents that hinder DNA extraction. Here, a rapid and efficient protocol for isolating total DNA from R. communis seeds was developed. The current method implies the use of repeated cycles of freeze/heat shock for the seed tissue to lyse the cells. DNA isolated with this protocol was successfully used as a template for PCR amplification of the internal transcribed spacer (ITS) region of the rRNA encoding gene that widely used for molecular identification of different plant species. As far to our knowledge, this study is the first one that report the efficient use of freeze/heat shock repeated cycles for isolation of a high-quality DNA from plant cells. The current protocol would support the subsequent analysis for seed lot purity analysis.
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| first_indexed | 2024-03-08T23:23:28Z |
| format | Article |
| id | doaj.art-1b60ec5d32ec4b55a734e5db80bef7ac |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2024-03-08T23:23:28Z |
| publishDate | 2023-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-1b60ec5d32ec4b55a734e5db80bef7ac2023-12-14T22:14:05ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412023-12-01119410.14720/aas.2023.119.4.16094A rapid and efficient DNA extraction method from high oily content seeds:Elham R. S. SOLIMAN0Cytogenetics and Molecular Genetics Unit, Botany and Microbiology Department, Faculty of Science, Helwan University, CairoRicinus communis seeds harbor high oil and polyphenolics contents that hinder DNA extraction. Here, a rapid and efficient protocol for isolating total DNA from R. communis seeds was developed. The current method implies the use of repeated cycles of freeze/heat shock for the seed tissue to lyse the cells. DNA isolated with this protocol was successfully used as a template for PCR amplification of the internal transcribed spacer (ITS) region of the rRNA encoding gene that widely used for molecular identification of different plant species. As far to our knowledge, this study is the first one that report the efficient use of freeze/heat shock repeated cycles for isolation of a high-quality DNA from plant cells. The current protocol would support the subsequent analysis for seed lot purity analysis. https://journals.uni-lj.si/aas/article/view/16094castor bean seedcell lysisheat shockITSPCRsequencing |
| spellingShingle | Elham R. S. SOLIMAN A rapid and efficient DNA extraction method from high oily content seeds: Acta Agriculturae Slovenica castor bean seed cell lysis heat shock ITS PCR sequencing |
| title | A rapid and efficient DNA extraction method from high oily content seeds: |
| title_full | A rapid and efficient DNA extraction method from high oily content seeds: |
| title_fullStr | A rapid and efficient DNA extraction method from high oily content seeds: |
| title_full_unstemmed | A rapid and efficient DNA extraction method from high oily content seeds: |
| title_short | A rapid and efficient DNA extraction method from high oily content seeds: |
| title_sort | rapid and efficient dna extraction method from high oily content seeds |
| topic | castor bean seed cell lysis heat shock ITS PCR sequencing |
| url | https://journals.uni-lj.si/aas/article/view/16094 |
| work_keys_str_mv | AT elhamrssoliman arapidandefficientdnaextractionmethodfromhighoilycontentseeds AT elhamrssoliman rapidandefficientdnaextractionmethodfromhighoilycontentseeds |