Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of Iran
The family Taeniidae is of great importance in the medical and veterinary fields, particularly in the tropics and subtropics. Identification of eggs of different Taenia spp. in the final host by morphological examination is difficult owing to their similarity. Therefore, a multiplex polymerase chain...
| Հիմնական հեղինակներ: | , , , , , , |
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| Ձևաչափ: | Հոդված |
| Լեզու: | English |
| Հրապարակվել է: |
Sciendo
2016-09-01
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| Շարք: | Helminthologia |
| Խորագրեր: | |
| Առցանց հասանելիություն: | https://doi.org/10.1515/helmin-2016-0017 |
| _version_ | 1827846830791065600 |
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| author | Rahimi M.T. Sarvi S. Daryani A. Sharif M. Ahmadpour E. Shokri A. Mizani A. |
| author_facet | Rahimi M.T. Sarvi S. Daryani A. Sharif M. Ahmadpour E. Shokri A. Mizani A. |
| author_sort | Rahimi M.T. |
| collection | DOAJ |
| description | The family Taeniidae is of great importance in the medical and veterinary fields, particularly in the tropics and subtropics. Identification of eggs of different Taenia spp. in the final host by morphological examination is difficult owing to their similarity. Therefore, a multiplex polymerase chain reaction (PCR) targeting a mitochondrial gene was applied to identify morphologically indistinguishable eggs. Fecal samples from 100 domestic dogs, from the Mazandaran province in Iran, were examined using the flotation/sieving method followed by multiplex PCR. Taeniid eggs were observed in 24 % samples, of which 12 %, 10 %, and 2 % were infected with Echinococcus granulosus, Taenia spp., and both E. granulosus and Taenia spp., respectively. E. multilocularis was absent in these samples. The prevalence of E. granulosus in the examined domestic dogs as definitive hosts in north of Iran was high (14 %). Therefore, people living in this region of Iran are in danger of acquiring hydatid cyst, which is a serious public health problem. |
| first_indexed | 2024-03-12T09:22:54Z |
| format | Article |
| id | doaj.art-1b64df6d4e79485e8ca19d3c5f78c6b0 |
| institution | Directory Open Access Journal |
| issn | 0440-6605 1336-9083 |
| language | English |
| last_indexed | 2024-03-12T09:22:54Z |
| publishDate | 2016-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Helminthologia |
| spelling | doaj.art-1b64df6d4e79485e8ca19d3c5f78c6b02023-09-02T14:23:23ZengSciendoHelminthologia0440-66051336-90832016-09-0153328528910.1515/helmin-2016-0017helmin-2016-0017Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of IranRahimi M.T.Sarvi S.0Daryani A.1Sharif M.2Ahmadpour E.3Shokri A.4Mizani A.5Toxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, IranToxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, IranToxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, IranInfectious and Tropical Diseases Research Centre, Tabriz University of Medical Sciences, Tabriz, IranToxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, IranToxoplasmosis Research Center, Mazandaran University of Medical Science, Sari, IranThe family Taeniidae is of great importance in the medical and veterinary fields, particularly in the tropics and subtropics. Identification of eggs of different Taenia spp. in the final host by morphological examination is difficult owing to their similarity. Therefore, a multiplex polymerase chain reaction (PCR) targeting a mitochondrial gene was applied to identify morphologically indistinguishable eggs. Fecal samples from 100 domestic dogs, from the Mazandaran province in Iran, were examined using the flotation/sieving method followed by multiplex PCR. Taeniid eggs were observed in 24 % samples, of which 12 %, 10 %, and 2 % were infected with Echinococcus granulosus, Taenia spp., and both E. granulosus and Taenia spp., respectively. E. multilocularis was absent in these samples. The prevalence of E. granulosus in the examined domestic dogs as definitive hosts in north of Iran was high (14 %). Therefore, people living in this region of Iran are in danger of acquiring hydatid cyst, which is a serious public health problem.https://doi.org/10.1515/helmin-2016-0017cestodataenia sppechinococcus granulosusmultiplex polymerase chain reaction (pcr) |
| spellingShingle | Rahimi M.T. Sarvi S. Daryani A. Sharif M. Ahmadpour E. Shokri A. Mizani A. Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of Iran Helminthologia cestoda taenia spp echinococcus granulosus multiplex polymerase chain reaction (pcr) |
| title | Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of Iran |
| title_full | Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of Iran |
| title_fullStr | Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of Iran |
| title_full_unstemmed | Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of Iran |
| title_short | Application of multiplex PCR for the simultaneous detection of Taenia spp. from domestic dogs in the north of Iran |
| title_sort | application of multiplex pcr for the simultaneous detection of taenia spp from domestic dogs in the north of iran |
| topic | cestoda taenia spp echinococcus granulosus multiplex polymerase chain reaction (pcr) |
| url | https://doi.org/10.1515/helmin-2016-0017 |
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