Mesenchymal stem cells have ameliorative effect on the colitis model via Nrf2/HO-1 pathway
ABSTRACT Purpose: To evaluate the ameliorative effect of mesenchymal stem cells (MSCs) on acetic acid colitis model via Nrf2/HO-1 pathway in rats. Methods: In this study, 30 rats were divided into three groups. Acute colitis was induced by rectal administration of 4% solution of acetic acid. MSCs...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2022-10-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000700200&tlng=en |
| _version_ | 1811194452856799232 |
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| author | Mehmet Fatih Bozkurt Muhammed Nasir Bhaya Cengiz Dibekoğlu Ayberk Akat Utku Ateş Oytun Erbaş |
| author_facet | Mehmet Fatih Bozkurt Muhammed Nasir Bhaya Cengiz Dibekoğlu Ayberk Akat Utku Ateş Oytun Erbaş |
| author_sort | Mehmet Fatih Bozkurt |
| collection | DOAJ |
| description | ABSTRACT Purpose: To evaluate the ameliorative effect of mesenchymal stem cells (MSCs) on acetic acid colitis model via Nrf2/HO-1 pathway in rats. Methods: In this study, 30 rats were divided into three groups. Acute colitis was induced by rectal administration of 4% solution of acetic acid. MSCs were injected intraperitoneally in the treatment group. Results: Increased levels of tumor necrosis factor-α (TNF-α), pentraxin-3, and malondialdehyde (MDA) in colitis group were revealed biochemically. Increased level of TNF-α and decreased levels of Nrf2 and interleukin-10 (IL-10) were observed in rectum tissues. Increased fibrous tissue proliferation, vascularization and inflammatory cell infiltration were described in the colitis group. Significant improvement was observed in MSCs treated group histopathologically. Increased immunopositivity of TNF-α, vascular endothelial growth factor (VEGF) and CD68 markers was observed in the colitis group cells, and decreased level of this positivity was observed in MSCs treated group. Conclusions: Biochemical, histopathological and immunohistochemical results strongly support the ameliorative effect of MSCs against acetic induced colitis model via Nrf2/HO-1 pathway in rats. |
| first_indexed | 2024-04-12T00:27:29Z |
| format | Article |
| id | doaj.art-1b6539064d9f4d1e93f7424bcf138eae |
| institution | Directory Open Access Journal |
| issn | 1678-2674 |
| language | English |
| last_indexed | 2024-04-12T00:27:29Z |
| publishDate | 2022-10-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-1b6539064d9f4d1e93f7424bcf138eae2022-12-22T03:55:27ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742022-10-0137710.1590/acb370704Mesenchymal stem cells have ameliorative effect on the colitis model via Nrf2/HO-1 pathwayMehmet Fatih Bozkurthttps://orcid.org/0000-0002-1669-0988Muhammed Nasir Bhayahttps://orcid.org/0000-0001-7696-3039Cengiz Dibekoğluhttps://orcid.org/0000-0001-7124-4385Ayberk Akathttps://orcid.org/0000-0001-6592-8369Utku Ateşhttps://orcid.org/0000-0002-7709-3626Oytun Erbaşhttps://orcid.org/0000-0001-5427-8428ABSTRACT Purpose: To evaluate the ameliorative effect of mesenchymal stem cells (MSCs) on acetic acid colitis model via Nrf2/HO-1 pathway in rats. Methods: In this study, 30 rats were divided into three groups. Acute colitis was induced by rectal administration of 4% solution of acetic acid. MSCs were injected intraperitoneally in the treatment group. Results: Increased levels of tumor necrosis factor-α (TNF-α), pentraxin-3, and malondialdehyde (MDA) in colitis group were revealed biochemically. Increased level of TNF-α and decreased levels of Nrf2 and interleukin-10 (IL-10) were observed in rectum tissues. Increased fibrous tissue proliferation, vascularization and inflammatory cell infiltration were described in the colitis group. Significant improvement was observed in MSCs treated group histopathologically. Increased immunopositivity of TNF-α, vascular endothelial growth factor (VEGF) and CD68 markers was observed in the colitis group cells, and decreased level of this positivity was observed in MSCs treated group. Conclusions: Biochemical, histopathological and immunohistochemical results strongly support the ameliorative effect of MSCs against acetic induced colitis model via Nrf2/HO-1 pathway in rats.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000700200&tlng=enInflammationAcetic AcidMesenchymal Stem CellsNF-E2-Related Factor 2Vascular Endothelial Growth Factor A |
| spellingShingle | Mehmet Fatih Bozkurt Muhammed Nasir Bhaya Cengiz Dibekoğlu Ayberk Akat Utku Ateş Oytun Erbaş Mesenchymal stem cells have ameliorative effect on the colitis model via Nrf2/HO-1 pathway Acta Cirúrgica Brasileira Inflammation Acetic Acid Mesenchymal Stem Cells NF-E2-Related Factor 2 Vascular Endothelial Growth Factor A |
| title | Mesenchymal stem cells have ameliorative effect on the colitis model via Nrf2/HO-1 pathway |
| title_full | Mesenchymal stem cells have ameliorative effect on the colitis model via Nrf2/HO-1 pathway |
| title_fullStr | Mesenchymal stem cells have ameliorative effect on the colitis model via Nrf2/HO-1 pathway |
| title_full_unstemmed | Mesenchymal stem cells have ameliorative effect on the colitis model via Nrf2/HO-1 pathway |
| title_short | Mesenchymal stem cells have ameliorative effect on the colitis model via Nrf2/HO-1 pathway |
| title_sort | mesenchymal stem cells have ameliorative effect on the colitis model via nrf2 ho 1 pathway |
| topic | Inflammation Acetic Acid Mesenchymal Stem Cells NF-E2-Related Factor 2 Vascular Endothelial Growth Factor A |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000700200&tlng=en |
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