Lysine-Grafted MCM-41 Silica as an Antibacterial Biomaterial
This paper proposes a facile strategy for the zwitterionization of bioceramics that is based on the direct incorporation of l-lysine amino acid via the ε-amino group onto mesoporous MCM-41 materials. Fourier transform infrared (FTIR) studies of lysine-grafted MCM-41 (MCM-LYS) simultaneously showed b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-09-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/4/4/80 |
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| author | María F. Villegas Lorena Garcia-Uriostegui Ofelia Rodríguez Isabel Izquierdo-Barba Antonio J. Salinas Guillermo Toriz María Vallet-Regí Ezequiel Delgado |
| author_facet | María F. Villegas Lorena Garcia-Uriostegui Ofelia Rodríguez Isabel Izquierdo-Barba Antonio J. Salinas Guillermo Toriz María Vallet-Regí Ezequiel Delgado |
| author_sort | María F. Villegas |
| collection | DOAJ |
| description | This paper proposes a facile strategy for the zwitterionization of bioceramics that is based on the direct incorporation of l-lysine amino acid via the ε-amino group onto mesoporous MCM-41 materials. Fourier transform infrared (FTIR) studies of lysine-grafted MCM-41 (MCM-LYS) simultaneously showed bands at 3080 and 1540 cm−1 and bands at 1625 and 1415 cm−1 corresponding to -NH3+/COO− pairs, which demonstrate the incorporation of the amino acid on the material surface keeping its zwitterionic character. Both elemental and thermogravimetric analyses showed that the amount of grafted lysine was 8 wt. % based on the bioceramic total weight. Moreover, MCM-LYS exhibited a reduction of adhesion of S. aureus and E. coli bacteria in 33% and 50%, respectively at physiological pH, as compared with pristine MCM-41. Biofilm studies onto surfaces showed that lysine functionalization elicited a reduction of the area covered by S. aureus biofilm from 42% to only 5% (88%). This research shows a simple and effective approach to chemically modify bioceramics using single amino acids that provides zwitterionic functionality, which is useful to develop new biomaterials that are able to resist bacterial adhesion. |
| first_indexed | 2024-03-12T10:34:19Z |
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| id | doaj.art-1b65fae96aff407bb31b598db8619323 |
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| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T10:34:19Z |
| publishDate | 2017-09-01 |
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| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-1b65fae96aff407bb31b598db86193232023-09-02T08:53:07ZengMDPI AGBioengineering2306-53542017-09-01448010.3390/bioengineering4040080bioengineering4040080Lysine-Grafted MCM-41 Silica as an Antibacterial BiomaterialMaría F. Villegas0Lorena Garcia-Uriostegui1Ofelia Rodríguez2Isabel Izquierdo-Barba3Antonio J. Salinas4Guillermo Toriz5María Vallet-Regí6Ezequiel Delgado7Departamento de Madera, Celulosa y Papel, Universidad de Guadalajara, Guadalajara 44100, MexicoCONACYT Research Fellow at Departamento de Madera, Celulosa y Papel, Universidad de Guadalajara, Guadalajara 44100, MexicoLaboratorio de Microbiología e Inocuidad de Alimentos, Universidad de Guadalajara, Guadalajara 44100, MexicoDepartamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, Plaza Ramón y Cajal s/n, 28040 Madrid, SpainDepartamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, Plaza Ramón y Cajal s/n, 28040 Madrid, SpainDepartamento de Madera, Celulosa y Papel, Universidad de Guadalajara, Guadalajara 44100, MexicoDepartamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, Plaza Ramón y Cajal s/n, 28040 Madrid, SpainDepartamento de Madera, Celulosa y Papel, Universidad de Guadalajara, Guadalajara 44100, MexicoThis paper proposes a facile strategy for the zwitterionization of bioceramics that is based on the direct incorporation of l-lysine amino acid via the ε-amino group onto mesoporous MCM-41 materials. Fourier transform infrared (FTIR) studies of lysine-grafted MCM-41 (MCM-LYS) simultaneously showed bands at 3080 and 1540 cm−1 and bands at 1625 and 1415 cm−1 corresponding to -NH3+/COO− pairs, which demonstrate the incorporation of the amino acid on the material surface keeping its zwitterionic character. Both elemental and thermogravimetric analyses showed that the amount of grafted lysine was 8 wt. % based on the bioceramic total weight. Moreover, MCM-LYS exhibited a reduction of adhesion of S. aureus and E. coli bacteria in 33% and 50%, respectively at physiological pH, as compared with pristine MCM-41. Biofilm studies onto surfaces showed that lysine functionalization elicited a reduction of the area covered by S. aureus biofilm from 42% to only 5% (88%). This research shows a simple and effective approach to chemically modify bioceramics using single amino acids that provides zwitterionic functionality, which is useful to develop new biomaterials that are able to resist bacterial adhesion.https://www.mdpi.com/2306-5354/4/4/80lysine graftingzwitterionizationmesoporous MCM-41 biomaterialantibacterial adhesionbiofilm formation |
| spellingShingle | María F. Villegas Lorena Garcia-Uriostegui Ofelia Rodríguez Isabel Izquierdo-Barba Antonio J. Salinas Guillermo Toriz María Vallet-Regí Ezequiel Delgado Lysine-Grafted MCM-41 Silica as an Antibacterial Biomaterial Bioengineering lysine grafting zwitterionization mesoporous MCM-41 biomaterial antibacterial adhesion biofilm formation |
| title | Lysine-Grafted MCM-41 Silica as an Antibacterial Biomaterial |
| title_full | Lysine-Grafted MCM-41 Silica as an Antibacterial Biomaterial |
| title_fullStr | Lysine-Grafted MCM-41 Silica as an Antibacterial Biomaterial |
| title_full_unstemmed | Lysine-Grafted MCM-41 Silica as an Antibacterial Biomaterial |
| title_short | Lysine-Grafted MCM-41 Silica as an Antibacterial Biomaterial |
| title_sort | lysine grafted mcm 41 silica as an antibacterial biomaterial |
| topic | lysine grafting zwitterionization mesoporous MCM-41 biomaterial antibacterial adhesion biofilm formation |
| url | https://www.mdpi.com/2306-5354/4/4/80 |
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