Safety assessment and gastrointestinal retention of orally administered cerium oxide nanoparticles in rats
Abstract Cerium oxide nanoparticles (CeO2 NPs, NM-212) are well-known for their catalytic properties and antioxidant potential, and have many applications in various industries, drug delivery, and cosmetic formulations. CeO2 NPs exhibit strong antimicrobial activity and can be used to efficiently re...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-03-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-54659-9 |
| _version_ | 1797266747554791424 |
|---|---|
| author | Hyoung-Yun Han Bo-Kyung Kim Jinhyung Rho Se-Myo Park Mi-Sun Choi Soojin Kim Min Beom Heo Young-Su Yang Jung-Hwa Oh Tae Geol Lee Seokjoo Yoon |
| author_facet | Hyoung-Yun Han Bo-Kyung Kim Jinhyung Rho Se-Myo Park Mi-Sun Choi Soojin Kim Min Beom Heo Young-Su Yang Jung-Hwa Oh Tae Geol Lee Seokjoo Yoon |
| author_sort | Hyoung-Yun Han |
| collection | DOAJ |
| description | Abstract Cerium oxide nanoparticles (CeO2 NPs, NM-212) are well-known for their catalytic properties and antioxidant potential, and have many applications in various industries, drug delivery, and cosmetic formulations. CeO2 NPs exhibit strong antimicrobial activity and can be used to efficiently remove pathogens from different environments. However, knowledge of the toxicological evaluation of CeO2 NPs is too limited to support their safe use. In this study, CeO2 NPs were orally administered to Sprague Dawley rats for 13 weeks at the doses of 0, 10, 100, and 1000 mg/kg bw/day, followed by a four week recovery period. The hematology values for the absolute and relative reticulocyte counts in male rats treated with 1000 mg/kg bw/day CeO2 NPs were lower than those in control rats. The clinical chemistry values for sodium and chloride in the treated male rat groups (100 and 1000 mg/kg/day) and total protein and calcium in the treated female rat groups (100 mg/kg/day) were higher than those in the control groups. However, these changes were not consistent in both sexes, and no abnormalities were found in the corresponding pathological findings. The results showed no adverse effects on any of the parameters assessed. CeO2 NPs accumulated in the jejunum, colon, and stomach wall of rats administered 1000 mg/kg CeO2 NPs for 90 days. However, these changes were not abnormal in the corresponding histopathological and immunohistochemical examinations. Therefore, 1000 mg/kg bw/day may be considered the “no observed adverse effect level” of CeO2 NPs (NM-212) in male and female SD rats under the present experimental conditions. |
| first_indexed | 2024-04-25T01:05:36Z |
| format | Article |
| id | doaj.art-1b66a2d4ca9b4c10b99a0a6a3ef55664 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-25T01:05:36Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1b66a2d4ca9b4c10b99a0a6a3ef556642024-03-10T12:12:31ZengNature PortfolioScientific Reports2045-23222024-03-0114111410.1038/s41598-024-54659-9Safety assessment and gastrointestinal retention of orally administered cerium oxide nanoparticles in ratsHyoung-Yun Han0Bo-Kyung Kim1Jinhyung Rho2Se-Myo Park3Mi-Sun Choi4Soojin Kim5Min Beom Heo6Young-Su Yang7Jung-Hwa Oh8Tae Geol Lee9Seokjoo Yoon10Department of Predictive Toxicology, Korea Institute of ToxicologyJeonbuk Branch Institute, Korea Institute of ToxicologyJeonbuk Branch Institute, Korea Institute of ToxicologyDepartment of Predictive Toxicology, Korea Institute of ToxicologyDepartment of Predictive Toxicology, Korea Institute of ToxicologyDepartment of Predictive Toxicology, Korea Institute of ToxicologyNanosafety Metrology Center, Korea Research Institute of Standards and Science (KRISS)Jeonbuk Branch Institute, Korea Institute of ToxicologyDepartment of Predictive Toxicology, Korea Institute of ToxicologyNanosafety Metrology Center, Korea Research Institute of Standards and Science (KRISS)Department of Predictive Toxicology, Korea Institute of ToxicologyAbstract Cerium oxide nanoparticles (CeO2 NPs, NM-212) are well-known for their catalytic properties and antioxidant potential, and have many applications in various industries, drug delivery, and cosmetic formulations. CeO2 NPs exhibit strong antimicrobial activity and can be used to efficiently remove pathogens from different environments. However, knowledge of the toxicological evaluation of CeO2 NPs is too limited to support their safe use. In this study, CeO2 NPs were orally administered to Sprague Dawley rats for 13 weeks at the doses of 0, 10, 100, and 1000 mg/kg bw/day, followed by a four week recovery period. The hematology values for the absolute and relative reticulocyte counts in male rats treated with 1000 mg/kg bw/day CeO2 NPs were lower than those in control rats. The clinical chemistry values for sodium and chloride in the treated male rat groups (100 and 1000 mg/kg/day) and total protein and calcium in the treated female rat groups (100 mg/kg/day) were higher than those in the control groups. However, these changes were not consistent in both sexes, and no abnormalities were found in the corresponding pathological findings. The results showed no adverse effects on any of the parameters assessed. CeO2 NPs accumulated in the jejunum, colon, and stomach wall of rats administered 1000 mg/kg CeO2 NPs for 90 days. However, these changes were not abnormal in the corresponding histopathological and immunohistochemical examinations. Therefore, 1000 mg/kg bw/day may be considered the “no observed adverse effect level” of CeO2 NPs (NM-212) in male and female SD rats under the present experimental conditions.https://doi.org/10.1038/s41598-024-54659-9Cerium dioxideNanoparticlesSubchronic toxicityOral toxicityNOAEL |
| spellingShingle | Hyoung-Yun Han Bo-Kyung Kim Jinhyung Rho Se-Myo Park Mi-Sun Choi Soojin Kim Min Beom Heo Young-Su Yang Jung-Hwa Oh Tae Geol Lee Seokjoo Yoon Safety assessment and gastrointestinal retention of orally administered cerium oxide nanoparticles in rats Scientific Reports Cerium dioxide Nanoparticles Subchronic toxicity Oral toxicity NOAEL |
| title | Safety assessment and gastrointestinal retention of orally administered cerium oxide nanoparticles in rats |
| title_full | Safety assessment and gastrointestinal retention of orally administered cerium oxide nanoparticles in rats |
| title_fullStr | Safety assessment and gastrointestinal retention of orally administered cerium oxide nanoparticles in rats |
| title_full_unstemmed | Safety assessment and gastrointestinal retention of orally administered cerium oxide nanoparticles in rats |
| title_short | Safety assessment and gastrointestinal retention of orally administered cerium oxide nanoparticles in rats |
| title_sort | safety assessment and gastrointestinal retention of orally administered cerium oxide nanoparticles in rats |
| topic | Cerium dioxide Nanoparticles Subchronic toxicity Oral toxicity NOAEL |
| url | https://doi.org/10.1038/s41598-024-54659-9 |
| work_keys_str_mv | AT hyoungyunhan safetyassessmentandgastrointestinalretentionoforallyadministeredceriumoxidenanoparticlesinrats AT bokyungkim safetyassessmentandgastrointestinalretentionoforallyadministeredceriumoxidenanoparticlesinrats AT jinhyungrho safetyassessmentandgastrointestinalretentionoforallyadministeredceriumoxidenanoparticlesinrats AT semyopark safetyassessmentandgastrointestinalretentionoforallyadministeredceriumoxidenanoparticlesinrats AT misunchoi safetyassessmentandgastrointestinalretentionoforallyadministeredceriumoxidenanoparticlesinrats AT soojinkim safetyassessmentandgastrointestinalretentionoforallyadministeredceriumoxidenanoparticlesinrats AT minbeomheo safetyassessmentandgastrointestinalretentionoforallyadministeredceriumoxidenanoparticlesinrats AT youngsuyang safetyassessmentandgastrointestinalretentionoforallyadministeredceriumoxidenanoparticlesinrats AT junghwaoh safetyassessmentandgastrointestinalretentionoforallyadministeredceriumoxidenanoparticlesinrats AT taegeollee safetyassessmentandgastrointestinalretentionoforallyadministeredceriumoxidenanoparticlesinrats AT seokjooyoon safetyassessmentandgastrointestinalretentionoforallyadministeredceriumoxidenanoparticlesinrats |