Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus
Abstract Objective Human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) are responsible for respiratory diseases, mostly in children. Despite the clinical and epidemiological similarities between these two pneumoviruses, they elicit different immune responses. This work aims to further...
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3541-0 |
| _version_ | 1819179590451462144 |
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| author | Ma. Del Rocio Baños-Lara Jovanny Zabaleta Jone Garai Melody Baddoo Antonieta Guerrero-Plata |
| author_facet | Ma. Del Rocio Baños-Lara Jovanny Zabaleta Jone Garai Melody Baddoo Antonieta Guerrero-Plata |
| author_sort | Ma. Del Rocio Baños-Lara |
| collection | DOAJ |
| description | Abstract Objective Human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) are responsible for respiratory diseases, mostly in children. Despite the clinical and epidemiological similarities between these two pneumoviruses, they elicit different immune responses. This work aims to further our understanding of the differential immune response induced by these respiratory viruses by determining the changes of small non-coding RNAs (miRNAs), which regulate gene expression and are involved in numerous cellular processes including the immune system. Results In the present study, we analyzed the expression of miRNA transcripts of human dendritic cells infected with RSV or HMPV by high throughput sequencing using Illumina sequencing technology. Further validation of miRNA expression by quantitative polymerase chain reaction indicated that HMPV infection up-regulated the expression of 2 miRNAs (hsa-miR-182-5p and hsa-miR-4634), while RSV infection induced significant expression of 3 miRNAs (hsa-miR-4448, hsa-miR-30a-5p and hsa-miR-4634). The predominant miRNA induced by both viruses was hsa-miR-4634. |
| first_indexed | 2024-12-22T22:00:52Z |
| format | Article |
| id | doaj.art-1b6cb9b86c6d4959aac3a5116b6f68f8 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-22T22:00:52Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-1b6cb9b86c6d4959aac3a5116b6f68f82022-12-21T18:11:08ZengBMCBMC Research Notes1756-05002018-07-011111710.1186/s13104-018-3541-0Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirusMa. Del Rocio Baños-Lara0Jovanny Zabaleta1Jone Garai2Melody Baddoo3Antonieta Guerrero-Plata4Department of Pathobiological Sciences, Louisiana State UniversityDepartment of Pediatrics, Louisiana State University Health Sciences CenterStanley S. Scott Cancer Center, Louisiana State University Health Sciences CenterTulane University School of MedicineDepartment of Pathobiological Sciences, Louisiana State UniversityAbstract Objective Human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) are responsible for respiratory diseases, mostly in children. Despite the clinical and epidemiological similarities between these two pneumoviruses, they elicit different immune responses. This work aims to further our understanding of the differential immune response induced by these respiratory viruses by determining the changes of small non-coding RNAs (miRNAs), which regulate gene expression and are involved in numerous cellular processes including the immune system. Results In the present study, we analyzed the expression of miRNA transcripts of human dendritic cells infected with RSV or HMPV by high throughput sequencing using Illumina sequencing technology. Further validation of miRNA expression by quantitative polymerase chain reaction indicated that HMPV infection up-regulated the expression of 2 miRNAs (hsa-miR-182-5p and hsa-miR-4634), while RSV infection induced significant expression of 3 miRNAs (hsa-miR-4448, hsa-miR-30a-5p and hsa-miR-4634). The predominant miRNA induced by both viruses was hsa-miR-4634.http://link.springer.com/article/10.1186/s13104-018-3541-0micro RNAPneumovirusHMPVRSVDendritic cellsDC |
| spellingShingle | Ma. Del Rocio Baños-Lara Jovanny Zabaleta Jone Garai Melody Baddoo Antonieta Guerrero-Plata Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus BMC Research Notes micro RNA Pneumovirus HMPV RSV Dendritic cells DC |
| title | Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus |
| title_full | Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus |
| title_fullStr | Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus |
| title_full_unstemmed | Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus |
| title_short | Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus |
| title_sort | comparative analysis of mirna profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus |
| topic | micro RNA Pneumovirus HMPV RSV Dendritic cells DC |
| url | http://link.springer.com/article/10.1186/s13104-018-3541-0 |
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