Enzymatic Hydrolysis of Copra Meal by Mannanase from Streptomyces sp. BF3.1 for The Production of Mannooligosaccharides
Copra meal contained high polysaccharide mannan. Mannanase Streptomyces sp. BF3.1 efficiently hydrolyzed copra meal to mannooligosaccharides. This research determined the optimum conditions of enzyme mannanase Streptomyces sp. BF3.1 to hydrolyze copra meal. The results of the hydrolysis products wer...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2015-04-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S197830191630078X |
| _version_ | 1818154429264691200 |
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| author | ARIANDI YOPI ANJA MERYANDINI |
| author_facet | ARIANDI YOPI ANJA MERYANDINI |
| author_sort | ARIANDI |
| collection | DOAJ |
| description | Copra meal contained high polysaccharide mannan. Mannanase Streptomyces sp. BF3.1 efficiently hydrolyzed copra meal to mannooligosaccharides. This research determined the optimum conditions of enzyme mannanase Streptomyces sp. BF3.1 to hydrolyze copra meal. The results of the hydrolysis products were analyzed concentrations of reducing sugars, total sugars and the degree of polymerization. In order to determine the type of product, mannooligosaccharides were analyzed by thin layer chromatography and high performance liquid chromatography. The mannanase had an optimum condition at 70 °C and pH 6. Optimum conditions of hydrolysis was 10% copra meal concentration with incubation time of 5 h at 30 °C which able to produce a variety of mannooligosaccharides products. Under such conditions, the yield of reducing sugar was 3.83 mg/mL with polymerization degree of 4. Analysis of mannooligosaccharides by thin layer chromatography and high performance liquid chromatography revealed mannobiose, mannotriose, mannotetrose, mannopentose, and mannoheksose. |
| first_indexed | 2024-12-11T14:26:22Z |
| format | Article |
| id | doaj.art-1b702c67a318417782a7a9c178c06ee2 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-12-11T14:26:22Z |
| publishDate | 2015-04-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-1b702c67a318417782a7a9c178c06ee22022-12-22T01:02:39ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192015-04-01222798610.4308/hjb.22.2.79Enzymatic Hydrolysis of Copra Meal by Mannanase from Streptomyces sp. BF3.1 for The Production of MannooligosaccharidesARIANDI0YOPI1ANJA MERYANDINI2Study Program of Biotechnology, Graduate School, Bogor Agricultural University, Darmaga Campus, Bogor 16680, IndonesiaBiocatalyst and Fermentation Laboratory, Research Center of Biotechnology, Indonesian Institute of Sciences, Cibinong 16911, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, IndonesiaCopra meal contained high polysaccharide mannan. Mannanase Streptomyces sp. BF3.1 efficiently hydrolyzed copra meal to mannooligosaccharides. This research determined the optimum conditions of enzyme mannanase Streptomyces sp. BF3.1 to hydrolyze copra meal. The results of the hydrolysis products were analyzed concentrations of reducing sugars, total sugars and the degree of polymerization. In order to determine the type of product, mannooligosaccharides were analyzed by thin layer chromatography and high performance liquid chromatography. The mannanase had an optimum condition at 70 °C and pH 6. Optimum conditions of hydrolysis was 10% copra meal concentration with incubation time of 5 h at 30 °C which able to produce a variety of mannooligosaccharides products. Under such conditions, the yield of reducing sugar was 3.83 mg/mL with polymerization degree of 4. Analysis of mannooligosaccharides by thin layer chromatography and high performance liquid chromatography revealed mannobiose, mannotriose, mannotetrose, mannopentose, and mannoheksose.http://www.sciencedirect.com/science/article/pii/S197830191630078Xcopra mealmannanasemannooligosaccharidesStreptomyces sp. |
| spellingShingle | ARIANDI YOPI ANJA MERYANDINI Enzymatic Hydrolysis of Copra Meal by Mannanase from Streptomyces sp. BF3.1 for The Production of Mannooligosaccharides Hayati Journal of Biosciences copra meal mannanase mannooligosaccharides Streptomyces sp. |
| title | Enzymatic Hydrolysis of Copra Meal by Mannanase from Streptomyces sp. BF3.1 for The Production of Mannooligosaccharides |
| title_full | Enzymatic Hydrolysis of Copra Meal by Mannanase from Streptomyces sp. BF3.1 for The Production of Mannooligosaccharides |
| title_fullStr | Enzymatic Hydrolysis of Copra Meal by Mannanase from Streptomyces sp. BF3.1 for The Production of Mannooligosaccharides |
| title_full_unstemmed | Enzymatic Hydrolysis of Copra Meal by Mannanase from Streptomyces sp. BF3.1 for The Production of Mannooligosaccharides |
| title_short | Enzymatic Hydrolysis of Copra Meal by Mannanase from Streptomyces sp. BF3.1 for The Production of Mannooligosaccharides |
| title_sort | enzymatic hydrolysis of copra meal by mannanase from streptomyces sp bf3 1 for the production of mannooligosaccharides |
| topic | copra meal mannanase mannooligosaccharides Streptomyces sp. |
| url | http://www.sciencedirect.com/science/article/pii/S197830191630078X |
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