Caffeoylquinic Acids and Flavonoids of Fringed Sagewort (<i>Artemisia frigida</i> Willd.): HPLC-DAD-ESI-QQQ-MS Profile, HPLC-DAD Quantification, in Vitro Digestion Stability, and Antioxidant Capacity
Fringed sagewort (<i>Artemisia frigida</i> Willd., Compositae family) is a well-known medicinal plant in Asian medical systems. Fifty-nine hydroxycinnamates and flavonoids have been found in <i>A. frigida</i> herbs of Siberian origin by high-performance liquid chromatography...
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MDPI AG
2019-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/8/307 |
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| author | Daniil N. Olennikov Nina I. Kashchenko Nadezhda K. Chirikova Aina G. Vasil’eva Aydan I. Gadimli Javanshir I. Isaev Cecile Vennos |
| author_facet | Daniil N. Olennikov Nina I. Kashchenko Nadezhda K. Chirikova Aina G. Vasil’eva Aydan I. Gadimli Javanshir I. Isaev Cecile Vennos |
| author_sort | Daniil N. Olennikov |
| collection | DOAJ |
| description | Fringed sagewort (<i>Artemisia frigida</i> Willd., Compositae family) is a well-known medicinal plant in Asian medical systems. Fifty-nine hydroxycinnamates and flavonoids have been found in <i>A. frigida</i> herbs of Siberian origin by high-performance liquid chromatography with diode array and electrospray triple quadrupole mass detection (HPLC-DAD-ESI-QQQ-MS). Their structures were determined after mass fragmentation analysis as caffeoylquinic acids, flavone <i>O</i>-/<i>C</i>-glycosides, flavones, and flavonol aglycones. Most of the discovered components were described in <i>A. frigida</i> for the first time. It was shown that flavonoids with different types of substitution have chemotaxonomic significance for species of <i>Artemisia</i> subsection Frigidae (section Absinthium). After HPLC-DAD quantification of 16 major phenolics in 21 Siberian populations of <i>A. frigida</i> and subsequent principal component analysis, we found substantial variation in the selected compounds, suggesting the existence of two geographical groups of <i>A. frigida</i>. The antioxidant activity of <i>A. frigida</i> herbal tea was determined using 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH<sup>•</sup>) and hydrophilic/lipophilic oxygen radical absorbance capacity (ORAC) assays and DPPH<sup>•</sup>-HPLC profiling, revealing it to be high. The effect of digestive media on the phenolic profile and antioxidant capacity of <i>A. frigida</i> herbal tea was assessed under simulated gastrointestinal digestion. We found a minor reduction in caffeoylquinic acid content and ORAC values, but remaining levels were satisfactory for antioxidant protection. These results suggest that <i>A. frigida</i> and its food derivate herbal tea could be recommended as new plant antioxidants rich in phenolics. |
| first_indexed | 2024-03-12T19:43:59Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T19:43:59Z |
| publishDate | 2019-08-01 |
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| series | Antioxidants |
| spelling | doaj.art-1b7b7c750b2f46afa0151591147c30002023-08-02T03:39:54ZengMDPI AGAntioxidants2076-39212019-08-018830710.3390/antiox8080307antiox8080307Caffeoylquinic Acids and Flavonoids of Fringed Sagewort (<i>Artemisia frigida</i> Willd.): HPLC-DAD-ESI-QQQ-MS Profile, HPLC-DAD Quantification, in Vitro Digestion Stability, and Antioxidant CapacityDaniil N. Olennikov0Nina I. Kashchenko1Nadezhda K. Chirikova2Aina G. Vasil’eva3Aydan I. Gadimli4Javanshir I. Isaev5Cecile Vennos6Laboratory of Medical and Biological Research, Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, 6 Sakh’yanovoy Street, Ulan-Ude 670047, RussiaLaboratory of Medical and Biological Research, Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, 6 Sakh’yanovoy Street, Ulan-Ude 670047, RussiaDepartment of Biochemistry and Biotechnology, North-Eastern Federal University, 58 Belinsky Street, Yakutsk 677027, RussiaDepartment of Biochemistry and Biotechnology, North-Eastern Federal University, 58 Belinsky Street, Yakutsk 677027, RussiaDepartment of Pharmacognosy, Azerbaijan Medical University, Anvar Gasimzade Street 14, Baku AZ1022, AzerbaijanDepartment of Pharmacognosy, Azerbaijan Medical University, Anvar Gasimzade Street 14, Baku AZ1022, AzerbaijanRegulatory and Medical Scientific Affairs, Padma AG, 1 Underfeldstrasse, CH-8340 Hinwil, SwitzerlandFringed sagewort (<i>Artemisia frigida</i> Willd., Compositae family) is a well-known medicinal plant in Asian medical systems. Fifty-nine hydroxycinnamates and flavonoids have been found in <i>A. frigida</i> herbs of Siberian origin by high-performance liquid chromatography with diode array and electrospray triple quadrupole mass detection (HPLC-DAD-ESI-QQQ-MS). Their structures were determined after mass fragmentation analysis as caffeoylquinic acids, flavone <i>O</i>-/<i>C</i>-glycosides, flavones, and flavonol aglycones. Most of the discovered components were described in <i>A. frigida</i> for the first time. It was shown that flavonoids with different types of substitution have chemotaxonomic significance for species of <i>Artemisia</i> subsection Frigidae (section Absinthium). After HPLC-DAD quantification of 16 major phenolics in 21 Siberian populations of <i>A. frigida</i> and subsequent principal component analysis, we found substantial variation in the selected compounds, suggesting the existence of two geographical groups of <i>A. frigida</i>. The antioxidant activity of <i>A. frigida</i> herbal tea was determined using 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH<sup>•</sup>) and hydrophilic/lipophilic oxygen radical absorbance capacity (ORAC) assays and DPPH<sup>•</sup>-HPLC profiling, revealing it to be high. The effect of digestive media on the phenolic profile and antioxidant capacity of <i>A. frigida</i> herbal tea was assessed under simulated gastrointestinal digestion. We found a minor reduction in caffeoylquinic acid content and ORAC values, but remaining levels were satisfactory for antioxidant protection. These results suggest that <i>A. frigida</i> and its food derivate herbal tea could be recommended as new plant antioxidants rich in phenolics.https://www.mdpi.com/2076-3921/8/8/307<i>Artemisia frigida</i>Compositae (Asteraceae)caffeoylquinic acidsflavonoidsHPLCmass spectrometrychemotaxonamyantioxidant capacityORAC |
| spellingShingle | Daniil N. Olennikov Nina I. Kashchenko Nadezhda K. Chirikova Aina G. Vasil’eva Aydan I. Gadimli Javanshir I. Isaev Cecile Vennos Caffeoylquinic Acids and Flavonoids of Fringed Sagewort (<i>Artemisia frigida</i> Willd.): HPLC-DAD-ESI-QQQ-MS Profile, HPLC-DAD Quantification, in Vitro Digestion Stability, and Antioxidant Capacity Antioxidants <i>Artemisia frigida</i> Compositae (Asteraceae) caffeoylquinic acids flavonoids HPLC mass spectrometry chemotaxonamy antioxidant capacity ORAC |
| title | Caffeoylquinic Acids and Flavonoids of Fringed Sagewort (<i>Artemisia frigida</i> Willd.): HPLC-DAD-ESI-QQQ-MS Profile, HPLC-DAD Quantification, in Vitro Digestion Stability, and Antioxidant Capacity |
| title_full | Caffeoylquinic Acids and Flavonoids of Fringed Sagewort (<i>Artemisia frigida</i> Willd.): HPLC-DAD-ESI-QQQ-MS Profile, HPLC-DAD Quantification, in Vitro Digestion Stability, and Antioxidant Capacity |
| title_fullStr | Caffeoylquinic Acids and Flavonoids of Fringed Sagewort (<i>Artemisia frigida</i> Willd.): HPLC-DAD-ESI-QQQ-MS Profile, HPLC-DAD Quantification, in Vitro Digestion Stability, and Antioxidant Capacity |
| title_full_unstemmed | Caffeoylquinic Acids and Flavonoids of Fringed Sagewort (<i>Artemisia frigida</i> Willd.): HPLC-DAD-ESI-QQQ-MS Profile, HPLC-DAD Quantification, in Vitro Digestion Stability, and Antioxidant Capacity |
| title_short | Caffeoylquinic Acids and Flavonoids of Fringed Sagewort (<i>Artemisia frigida</i> Willd.): HPLC-DAD-ESI-QQQ-MS Profile, HPLC-DAD Quantification, in Vitro Digestion Stability, and Antioxidant Capacity |
| title_sort | caffeoylquinic acids and flavonoids of fringed sagewort i artemisia frigida i willd hplc dad esi qqq ms profile hplc dad quantification in vitro digestion stability and antioxidant capacity |
| topic | <i>Artemisia frigida</i> Compositae (Asteraceae) caffeoylquinic acids flavonoids HPLC mass spectrometry chemotaxonamy antioxidant capacity ORAC |
| url | https://www.mdpi.com/2076-3921/8/8/307 |
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