Developmental programming modulates olfactory behavior in C. elegans via endogenous RNAi pathways
Environmental stress during early development can impact adult phenotypes via programmed changes in gene expression. C. elegans larvae respond to environmental stress by entering the stress-resistant dauer diapause pathway and resume development once conditions improve (postdauers). Here we show tha...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2016-06-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/11642 |
| _version_ | 1818018552188239872 |
|---|---|
| author | Jennie R Sims Maria C Ow Mailyn A Nishiguchi Kyuhyung Kim Piali Sengupta Sarah E Hall |
| author_facet | Jennie R Sims Maria C Ow Mailyn A Nishiguchi Kyuhyung Kim Piali Sengupta Sarah E Hall |
| author_sort | Jennie R Sims |
| collection | DOAJ |
| description | Environmental stress during early development can impact adult phenotypes via programmed changes in gene expression. C. elegans larvae respond to environmental stress by entering the stress-resistant dauer diapause pathway and resume development once conditions improve (postdauers). Here we show that the osm-9 TRPV channel gene is a target of developmental programming and is down-regulated specifically in the ADL chemosensory neurons of postdauer adults, resulting in a corresponding altered olfactory behavior that is mediated by ADL in an OSM-9-dependent manner. We identify a cis-acting motif bound by the DAF-3 SMAD and ZFP-1 (AF10) proteins that is necessary for the differential regulation of osm-9, and demonstrate that both chromatin remodeling and endo-siRNA pathways are major contributors to the transcriptional silencing of the osm-9 locus. This work describes an elegant mechanism by which developmental experience influences adult phenotypes by establishing and maintaining transcriptional changes via RNAi and chromatin remodeling pathways. |
| first_indexed | 2024-04-14T07:41:06Z |
| format | Article |
| id | doaj.art-1b80abdd677942baa0f2da7012ba00c9 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:41:06Z |
| publishDate | 2016-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-1b80abdd677942baa0f2da7012ba00c92022-12-22T02:05:29ZengeLife Sciences Publications LtdeLife2050-084X2016-06-01510.7554/eLife.11642Developmental programming modulates olfactory behavior in C. elegans via endogenous RNAi pathwaysJennie R Sims0Maria C Ow1Mailyn A Nishiguchi2Kyuhyung Kim3Piali Sengupta4Sarah E Hall5https://orcid.org/0000-0002-8536-4000Department of Biology, Syracuse University, Syracuse, United StatesDepartment of Biology, Syracuse University, Syracuse, United StatesDepartment of Biology, Syracuse University, Syracuse, United StatesDepartment of Brain and Cognitive Sciences, Daegu Gyeongbuk Institute of Science and Technology, Daegu, Republic of KoreaNational Center for Behavioral Genomics, Department of Biology, Brandeis University, Waltham, United StatesDepartment of Biology, Syracuse University, Syracuse, United StatesEnvironmental stress during early development can impact adult phenotypes via programmed changes in gene expression. C. elegans larvae respond to environmental stress by entering the stress-resistant dauer diapause pathway and resume development once conditions improve (postdauers). Here we show that the osm-9 TRPV channel gene is a target of developmental programming and is down-regulated specifically in the ADL chemosensory neurons of postdauer adults, resulting in a corresponding altered olfactory behavior that is mediated by ADL in an OSM-9-dependent manner. We identify a cis-acting motif bound by the DAF-3 SMAD and ZFP-1 (AF10) proteins that is necessary for the differential regulation of osm-9, and demonstrate that both chromatin remodeling and endo-siRNA pathways are major contributors to the transcriptional silencing of the osm-9 locus. This work describes an elegant mechanism by which developmental experience influences adult phenotypes by establishing and maintaining transcriptional changes via RNAi and chromatin remodeling pathways.https://elifesciences.org/articles/11642postdauerdauerTGF-betaRNAichromatinosm-9 |
| spellingShingle | Jennie R Sims Maria C Ow Mailyn A Nishiguchi Kyuhyung Kim Piali Sengupta Sarah E Hall Developmental programming modulates olfactory behavior in C. elegans via endogenous RNAi pathways eLife postdauer dauer TGF-beta RNAi chromatin osm-9 |
| title | Developmental programming modulates olfactory behavior in C. elegans via endogenous RNAi pathways |
| title_full | Developmental programming modulates olfactory behavior in C. elegans via endogenous RNAi pathways |
| title_fullStr | Developmental programming modulates olfactory behavior in C. elegans via endogenous RNAi pathways |
| title_full_unstemmed | Developmental programming modulates olfactory behavior in C. elegans via endogenous RNAi pathways |
| title_short | Developmental programming modulates olfactory behavior in C. elegans via endogenous RNAi pathways |
| title_sort | developmental programming modulates olfactory behavior in c elegans via endogenous rnai pathways |
| topic | postdauer dauer TGF-beta RNAi chromatin osm-9 |
| url | https://elifesciences.org/articles/11642 |
| work_keys_str_mv | AT jenniersims developmentalprogrammingmodulatesolfactorybehaviorincelegansviaendogenousrnaipathways AT mariacow developmentalprogrammingmodulatesolfactorybehaviorincelegansviaendogenousrnaipathways AT mailynanishiguchi developmentalprogrammingmodulatesolfactorybehaviorincelegansviaendogenousrnaipathways AT kyuhyungkim developmentalprogrammingmodulatesolfactorybehaviorincelegansviaendogenousrnaipathways AT pialisengupta developmentalprogrammingmodulatesolfactorybehaviorincelegansviaendogenousrnaipathways AT sarahehall developmentalprogrammingmodulatesolfactorybehaviorincelegansviaendogenousrnaipathways |