A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast
Cellular aging is a multifactorial process that is characterized by a decline in homeostatic capacity, best described at the molecular level. Physicochemical properties such as pH and macromolecular crowding are essential to all molecular processes in cells and require maintenance. Whether a drift i...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-09-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/54707 |
| _version_ | 1811202612002816000 |
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| author | Sara N Mouton David J Thaller Matthew M Crane Irina L Rempel Owen T Terpstra Anton Steen Matt Kaeberlein C Patrick Lusk Arnold J Boersma Liesbeth M Veenhoff |
| author_facet | Sara N Mouton David J Thaller Matthew M Crane Irina L Rempel Owen T Terpstra Anton Steen Matt Kaeberlein C Patrick Lusk Arnold J Boersma Liesbeth M Veenhoff |
| author_sort | Sara N Mouton |
| collection | DOAJ |
| description | Cellular aging is a multifactorial process that is characterized by a decline in homeostatic capacity, best described at the molecular level. Physicochemical properties such as pH and macromolecular crowding are essential to all molecular processes in cells and require maintenance. Whether a drift in physicochemical properties contributes to the overall decline of homeostasis in aging is not known. Here, we show that the cytosol of yeast cells acidifies modestly in early aging and sharply after senescence. Using a macromolecular crowding sensor optimized for long-term FRET measurements, we show that crowding is rather stable and that the stability of crowding is a stronger predictor for lifespan than the absolute crowding levels. Additionally, in aged cells, we observe drastic changes in organellar volume, leading to crowding on the micrometer scale, which we term organellar crowding. Our measurements provide an initial framework of physicochemical parameters of replicatively aged yeast cells. |
| first_indexed | 2024-04-12T02:41:42Z |
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| id | doaj.art-1b80c526f8be4038a0f0386d26ecaf5c |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:41:42Z |
| publishDate | 2020-09-01 |
| publisher | eLife Sciences Publications Ltd |
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| spelling | doaj.art-1b80c526f8be4038a0f0386d26ecaf5c2022-12-22T03:51:18ZengeLife Sciences Publications LtdeLife2050-084X2020-09-01910.7554/eLife.54707A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeastSara N Mouton0https://orcid.org/0000-0001-9429-3788David J Thaller1https://orcid.org/0000-0003-3577-5562Matthew M Crane2https://orcid.org/0000-0002-6234-0954Irina L Rempel3https://orcid.org/0000-0003-4655-5311Owen T Terpstra4https://orcid.org/0000-0002-8767-4061Anton Steen5https://orcid.org/0000-0002-1064-6038Matt Kaeberlein6https://orcid.org/0000-0002-1311-3421C Patrick Lusk7https://orcid.org/0000-0003-4703-0533Arnold J Boersma8https://orcid.org/0000-0002-3714-5938Liesbeth M Veenhoff9https://orcid.org/0000-0002-0158-4728European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsDepartment of Cell Biology, Yale School of Medicine, New Haven, United StatesDepartment of Pathology, School of Medicine, University of Washington, Seattle, United StatesEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsDepartment of Pathology, School of Medicine, University of Washington, Seattle, United StatesDepartment of Cell Biology, Yale School of Medicine, New Haven, United StatesDWI-Leibniz Institute for Interactive Materials, Aachen, GermanyEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, NetherlandsCellular aging is a multifactorial process that is characterized by a decline in homeostatic capacity, best described at the molecular level. Physicochemical properties such as pH and macromolecular crowding are essential to all molecular processes in cells and require maintenance. Whether a drift in physicochemical properties contributes to the overall decline of homeostasis in aging is not known. Here, we show that the cytosol of yeast cells acidifies modestly in early aging and sharply after senescence. Using a macromolecular crowding sensor optimized for long-term FRET measurements, we show that crowding is rather stable and that the stability of crowding is a stronger predictor for lifespan than the absolute crowding levels. Additionally, in aged cells, we observe drastic changes in organellar volume, leading to crowding on the micrometer scale, which we term organellar crowding. Our measurements provide an initial framework of physicochemical parameters of replicatively aged yeast cells.https://elifesciences.org/articles/54707agingpHcrowdingFRET sensor |
| spellingShingle | Sara N Mouton David J Thaller Matthew M Crane Irina L Rempel Owen T Terpstra Anton Steen Matt Kaeberlein C Patrick Lusk Arnold J Boersma Liesbeth M Veenhoff A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast eLife aging pH crowding FRET sensor |
| title | A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast |
| title_full | A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast |
| title_fullStr | A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast |
| title_full_unstemmed | A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast |
| title_short | A physicochemical perspective of aging from single-cell analysis of pH, macromolecular and organellar crowding in yeast |
| title_sort | physicochemical perspective of aging from single cell analysis of ph macromolecular and organellar crowding in yeast |
| topic | aging pH crowding FRET sensor |
| url | https://elifesciences.org/articles/54707 |
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