Seedlessness Trait and Genome Editing—A Review

Parthenocarpy and stenospermocarpy are the two mechanisms underlying the seedless fruit set program. Seedless fruit occurs naturally and can be produced using hormone application, crossbreeding, or ploidy breeding. However, the two types of breeding are time-consuming and sometimes ineffective due t...

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Main Authors: Md Moniruzzaman, Ahmed G. Darwish, Ahmed Ismail, Ashraf El-kereamy, Violeta Tsolova, Islam El-Sharkawy
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/24/6/5660
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author Md Moniruzzaman
Ahmed G. Darwish
Ahmed Ismail
Ashraf El-kereamy
Violeta Tsolova
Islam El-Sharkawy
author_facet Md Moniruzzaman
Ahmed G. Darwish
Ahmed Ismail
Ashraf El-kereamy
Violeta Tsolova
Islam El-Sharkawy
author_sort Md Moniruzzaman
collection DOAJ
description Parthenocarpy and stenospermocarpy are the two mechanisms underlying the seedless fruit set program. Seedless fruit occurs naturally and can be produced using hormone application, crossbreeding, or ploidy breeding. However, the two types of breeding are time-consuming and sometimes ineffective due to interspecies hybridization barriers or the absence of appropriate parental genotypes to use in the breeding process. The genetic engineering approach provides a better prospect, which can be explored based on an understanding of the genetic causes underlying the seedlessness trait. For instance, CRISPR/Cas is a comprehensive and precise technology. The prerequisite for using the strategy to induce seedlessness is identifying the crucial master gene or transcription factor liable for seed formation/development. In this review, we primarily explored the seedlessness mechanisms and identified the potential candidate genes underlying seed development. We also discussed the CRISPR/Cas-mediated genome editing approaches and their improvements.
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spelling doaj.art-1b818072afeb4d608028951e5068e6c82023-11-17T11:37:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246566010.3390/ijms24065660Seedlessness Trait and Genome Editing—A ReviewMd Moniruzzaman0Ahmed G. Darwish1Ahmed Ismail2Ashraf El-kereamy3Violeta Tsolova4Islam El-Sharkawy5Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USACenter for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USACenter for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USADepartment of Botany and Plant Sciences, University of California Riverside, Riverside, CA 92521, USACenter for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USACenter for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USAParthenocarpy and stenospermocarpy are the two mechanisms underlying the seedless fruit set program. Seedless fruit occurs naturally and can be produced using hormone application, crossbreeding, or ploidy breeding. However, the two types of breeding are time-consuming and sometimes ineffective due to interspecies hybridization barriers or the absence of appropriate parental genotypes to use in the breeding process. The genetic engineering approach provides a better prospect, which can be explored based on an understanding of the genetic causes underlying the seedlessness trait. For instance, CRISPR/Cas is a comprehensive and precise technology. The prerequisite for using the strategy to induce seedlessness is identifying the crucial master gene or transcription factor liable for seed formation/development. In this review, we primarily explored the seedlessness mechanisms and identified the potential candidate genes underlying seed development. We also discussed the CRISPR/Cas-mediated genome editing approaches and their improvements.https://www.mdpi.com/1422-0067/24/6/5660CRISPR/Casgenome editingmolecular breedingovule abortionparthenocarpyseedlessness
spellingShingle Md Moniruzzaman
Ahmed G. Darwish
Ahmed Ismail
Ashraf El-kereamy
Violeta Tsolova
Islam El-Sharkawy
Seedlessness Trait and Genome Editing—A Review
International Journal of Molecular Sciences
CRISPR/Cas
genome editing
molecular breeding
ovule abortion
parthenocarpy
seedlessness
title Seedlessness Trait and Genome Editing—A Review
title_full Seedlessness Trait and Genome Editing—A Review
title_fullStr Seedlessness Trait and Genome Editing—A Review
title_full_unstemmed Seedlessness Trait and Genome Editing—A Review
title_short Seedlessness Trait and Genome Editing—A Review
title_sort seedlessness trait and genome editing a review
topic CRISPR/Cas
genome editing
molecular breeding
ovule abortion
parthenocarpy
seedlessness
url https://www.mdpi.com/1422-0067/24/6/5660
work_keys_str_mv AT mdmoniruzzaman seedlessnesstraitandgenomeeditingareview
AT ahmedgdarwish seedlessnesstraitandgenomeeditingareview
AT ahmedismail seedlessnesstraitandgenomeeditingareview
AT ashrafelkereamy seedlessnesstraitandgenomeeditingareview
AT violetatsolova seedlessnesstraitandgenomeeditingareview
AT islamelsharkawy seedlessnesstraitandgenomeeditingareview