Seedlessness Trait and Genome Editing—A Review
Parthenocarpy and stenospermocarpy are the two mechanisms underlying the seedless fruit set program. Seedless fruit occurs naturally and can be produced using hormone application, crossbreeding, or ploidy breeding. However, the two types of breeding are time-consuming and sometimes ineffective due t...
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MDPI AG
2023-03-01
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Series: | International Journal of Molecular Sciences |
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Online Access: | https://www.mdpi.com/1422-0067/24/6/5660 |
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author | Md Moniruzzaman Ahmed G. Darwish Ahmed Ismail Ashraf El-kereamy Violeta Tsolova Islam El-Sharkawy |
author_facet | Md Moniruzzaman Ahmed G. Darwish Ahmed Ismail Ashraf El-kereamy Violeta Tsolova Islam El-Sharkawy |
author_sort | Md Moniruzzaman |
collection | DOAJ |
description | Parthenocarpy and stenospermocarpy are the two mechanisms underlying the seedless fruit set program. Seedless fruit occurs naturally and can be produced using hormone application, crossbreeding, or ploidy breeding. However, the two types of breeding are time-consuming and sometimes ineffective due to interspecies hybridization barriers or the absence of appropriate parental genotypes to use in the breeding process. The genetic engineering approach provides a better prospect, which can be explored based on an understanding of the genetic causes underlying the seedlessness trait. For instance, CRISPR/Cas is a comprehensive and precise technology. The prerequisite for using the strategy to induce seedlessness is identifying the crucial master gene or transcription factor liable for seed formation/development. In this review, we primarily explored the seedlessness mechanisms and identified the potential candidate genes underlying seed development. We also discussed the CRISPR/Cas-mediated genome editing approaches and their improvements. |
first_indexed | 2024-03-11T06:24:44Z |
format | Article |
id | doaj.art-1b818072afeb4d608028951e5068e6c8 |
institution | Directory Open Access Journal |
issn | 1661-6596 1422-0067 |
language | English |
last_indexed | 2024-03-11T06:24:44Z |
publishDate | 2023-03-01 |
publisher | MDPI AG |
record_format | Article |
series | International Journal of Molecular Sciences |
spelling | doaj.art-1b818072afeb4d608028951e5068e6c82023-11-17T11:37:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246566010.3390/ijms24065660Seedlessness Trait and Genome Editing—A ReviewMd Moniruzzaman0Ahmed G. Darwish1Ahmed Ismail2Ashraf El-kereamy3Violeta Tsolova4Islam El-Sharkawy5Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USACenter for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USACenter for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USADepartment of Botany and Plant Sciences, University of California Riverside, Riverside, CA 92521, USACenter for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USACenter for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences, Florida A&M University, Tallahassee, FL 32308, USAParthenocarpy and stenospermocarpy are the two mechanisms underlying the seedless fruit set program. Seedless fruit occurs naturally and can be produced using hormone application, crossbreeding, or ploidy breeding. However, the two types of breeding are time-consuming and sometimes ineffective due to interspecies hybridization barriers or the absence of appropriate parental genotypes to use in the breeding process. The genetic engineering approach provides a better prospect, which can be explored based on an understanding of the genetic causes underlying the seedlessness trait. For instance, CRISPR/Cas is a comprehensive and precise technology. The prerequisite for using the strategy to induce seedlessness is identifying the crucial master gene or transcription factor liable for seed formation/development. In this review, we primarily explored the seedlessness mechanisms and identified the potential candidate genes underlying seed development. We also discussed the CRISPR/Cas-mediated genome editing approaches and their improvements.https://www.mdpi.com/1422-0067/24/6/5660CRISPR/Casgenome editingmolecular breedingovule abortionparthenocarpyseedlessness |
spellingShingle | Md Moniruzzaman Ahmed G. Darwish Ahmed Ismail Ashraf El-kereamy Violeta Tsolova Islam El-Sharkawy Seedlessness Trait and Genome Editing—A Review International Journal of Molecular Sciences CRISPR/Cas genome editing molecular breeding ovule abortion parthenocarpy seedlessness |
title | Seedlessness Trait and Genome Editing—A Review |
title_full | Seedlessness Trait and Genome Editing—A Review |
title_fullStr | Seedlessness Trait and Genome Editing—A Review |
title_full_unstemmed | Seedlessness Trait and Genome Editing—A Review |
title_short | Seedlessness Trait and Genome Editing—A Review |
title_sort | seedlessness trait and genome editing a review |
topic | CRISPR/Cas genome editing molecular breeding ovule abortion parthenocarpy seedlessness |
url | https://www.mdpi.com/1422-0067/24/6/5660 |
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