Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over Endothelial Colony-Forming Cells
Cardiovascular diseases remain the leading cause of death worldwide, mainly triggered by the formation of atherosclerotic plaques that reduce blood flow. Angiogenic cell therapy based on endothelial colony forming cells (ECFCs) constitutes a promising alternative to promote vascular revascularizatio...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/5/851 |
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| author | Ismael Sánchez-Gomar Josefa Benítez-Camacho Cristina Cejudo-Bastante Lourdes Casas Rafael Moreno-Luna Casimiro Mantell Mª Carmen Durán-Ruiz |
| author_facet | Ismael Sánchez-Gomar Josefa Benítez-Camacho Cristina Cejudo-Bastante Lourdes Casas Rafael Moreno-Luna Casimiro Mantell Mª Carmen Durán-Ruiz |
| author_sort | Ismael Sánchez-Gomar |
| collection | DOAJ |
| description | Cardiovascular diseases remain the leading cause of death worldwide, mainly triggered by the formation of atherosclerotic plaques that reduce blood flow. Angiogenic cell therapy based on endothelial colony forming cells (ECFCs) constitutes a promising alternative to promote vascular revascularization; however, under the oxidative environment that prevails in ischemic areas, these cells become impaired. Thus, it is necessary to investigate strategies to enhance their regenerative properties. Antioxidant substances, such as polyphenols, have been shown to be useful for this purpose. In the current study we evaluated the potential of mango leaves, olive leaves and red grape pomace extracts, rich in polyphenols, to promote ECFC reparative effects. For this, aqueous and ethanolic extracts of the aforementioned raw materials were obtained by pressurized liquid extraction (PLE). After evaluating the polyphenol content and the antioxidant activity, in vitro assays were carried out, and we found that ethanolic extracts at low concentrations improved angiogenic capacities of ECFCs and reduced proliferation, apoptosis, and the inflammatory response of these cells. Overall, mango leaves ethanolic extract provided the most promising results, but all three extracts ameliorated the functionality of ECFCs. |
| first_indexed | 2024-03-10T03:26:03Z |
| format | Article |
| id | doaj.art-1b83d9fc52954b318e99700f14f00ceb |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:26:03Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-1b83d9fc52954b318e99700f14f00ceb2023-11-23T09:50:44ZengMDPI AGAntioxidants2076-39212022-04-0111585110.3390/antiox11050851Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over Endothelial Colony-Forming CellsIsmael Sánchez-Gomar0Josefa Benítez-Camacho1Cristina Cejudo-Bastante2Lourdes Casas3Rafael Moreno-Luna4Casimiro Mantell5Mª Carmen Durán-Ruiz6Biomedicine, Biotechnology and Public Health Department, University of Cadiz, 11002 Cadiz, SpainBiomedicine, Biotechnology and Public Health Department, University of Cadiz, 11002 Cadiz, SpainChemical Engineering and Food Technology Department, Science Faculty, Wine and Agrifood Research Institute (IVAGRO), University of Cadiz, 11519 Cadiz, SpainChemical Engineering and Food Technology Department, Science Faculty, Wine and Agrifood Research Institute (IVAGRO), University of Cadiz, 11519 Cadiz, SpainLaboratory of Neuroinflammation, National Paraplegics Hospital, SESCAM, 45071 Toledo, SpainChemical Engineering and Food Technology Department, Science Faculty, Wine and Agrifood Research Institute (IVAGRO), University of Cadiz, 11519 Cadiz, SpainBiomedicine, Biotechnology and Public Health Department, University of Cadiz, 11002 Cadiz, SpainCardiovascular diseases remain the leading cause of death worldwide, mainly triggered by the formation of atherosclerotic plaques that reduce blood flow. Angiogenic cell therapy based on endothelial colony forming cells (ECFCs) constitutes a promising alternative to promote vascular revascularization; however, under the oxidative environment that prevails in ischemic areas, these cells become impaired. Thus, it is necessary to investigate strategies to enhance their regenerative properties. Antioxidant substances, such as polyphenols, have been shown to be useful for this purpose. In the current study we evaluated the potential of mango leaves, olive leaves and red grape pomace extracts, rich in polyphenols, to promote ECFC reparative effects. For this, aqueous and ethanolic extracts of the aforementioned raw materials were obtained by pressurized liquid extraction (PLE). After evaluating the polyphenol content and the antioxidant activity, in vitro assays were carried out, and we found that ethanolic extracts at low concentrations improved angiogenic capacities of ECFCs and reduced proliferation, apoptosis, and the inflammatory response of these cells. Overall, mango leaves ethanolic extract provided the most promising results, but all three extracts ameliorated the functionality of ECFCs.https://www.mdpi.com/2076-3921/11/5/851ECFCsmango leavesolive leavesgrape pomaceangiogenesisproliferation |
| spellingShingle | Ismael Sánchez-Gomar Josefa Benítez-Camacho Cristina Cejudo-Bastante Lourdes Casas Rafael Moreno-Luna Casimiro Mantell Mª Carmen Durán-Ruiz Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over Endothelial Colony-Forming Cells Antioxidants ECFCs mango leaves olive leaves grape pomace angiogenesis proliferation |
| title | Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over Endothelial Colony-Forming Cells |
| title_full | Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over Endothelial Colony-Forming Cells |
| title_fullStr | Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over Endothelial Colony-Forming Cells |
| title_full_unstemmed | Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over Endothelial Colony-Forming Cells |
| title_short | Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over Endothelial Colony-Forming Cells |
| title_sort | pro angiogenic effects of natural antioxidants extracted from mango leaf olive leaf and red grape pomace over endothelial colony forming cells |
| topic | ECFCs mango leaves olive leaves grape pomace angiogenesis proliferation |
| url | https://www.mdpi.com/2076-3921/11/5/851 |
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