Incorporation of Human Recombinant Tropoelastin into Silk Fibroin Membranes with the View to Repairing Bruch’s Membrane
Bombyx mori silk fibroin membranes provide a potential delivery vehicle for both cells and extracellular matrix (ECM) components into diseased or injured tissues. We have previously demonstrated the feasibility of growing retinal pigment epithelial cells (RPE) on fibroin membranes with the view to r...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-09-01
|
| Series: | Journal of Functional Biomaterials |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-4983/6/3/946 |
| _version_ | 1818013973994274816 |
|---|---|
| author | Audra M. A. Shadforth Shuko Suzuki Raphaelle Alzonne Grant A. Edwards Neil A. Richardson Traian V. Chirila Damien G. Harkin |
| author_facet | Audra M. A. Shadforth Shuko Suzuki Raphaelle Alzonne Grant A. Edwards Neil A. Richardson Traian V. Chirila Damien G. Harkin |
| author_sort | Audra M. A. Shadforth |
| collection | DOAJ |
| description | Bombyx mori silk fibroin membranes provide a potential delivery vehicle for both cells and extracellular matrix (ECM) components into diseased or injured tissues. We have previously demonstrated the feasibility of growing retinal pigment epithelial cells (RPE) on fibroin membranes with the view to repairing the retina of patients afflicted with age-related macular degeneration (AMD). The goal of the present study was to investigate the feasibility of incorporating the ECM component elastin, in the form of human recombinant tropoelastin, into these same membranes. Two basic strategies were explored: (1) membranes prepared from blended solutions of fibroin and tropoelastin; and (2) layered constructs prepared from sequentially cast solutions of fibroin, tropoelastin, and fibroin. Optimal conditions for RPE attachment were achieved using a tropoelastin-fibroin blend ratio of 10 to 90 parts by weight. Retention of tropoelastin within the blend and layered constructs was confirmed by immunolabelling and Fourier-transform infrared spectroscopy (FTIR). In the layered constructs, the bulk of tropoelastin was apparently absorbed into the initially cast fibroin layer. Blend membranes displayed higher elastic modulus, percentage elongation, and tensile strength (p < 0.01) when compared to the layered constructs. RPE cell response to fibroin membranes was not affected by the presence of tropoelastin. These findings support the potential use of fibroin membranes for the co-delivery of RPE cells and tropoelastin. |
| first_indexed | 2024-04-14T06:39:53Z |
| format | Article |
| id | doaj.art-1b86aab778dc41a8a7b4cd4b3a74d53a |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-04-14T06:39:53Z |
| publishDate | 2015-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-1b86aab778dc41a8a7b4cd4b3a74d53a2022-12-22T02:07:21ZengMDPI AGJournal of Functional Biomaterials2079-49832015-09-016394696210.3390/jfb6030946jfb6030946Incorporation of Human Recombinant Tropoelastin into Silk Fibroin Membranes with the View to Repairing Bruch’s MembraneAudra M. A. Shadforth0Shuko Suzuki1Raphaelle Alzonne2Grant A. Edwards3Neil A. Richardson4Traian V. Chirila5Damien G. Harkin6Queensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, AustraliaQueensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, AustraliaQueensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, AustraliaAustralian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, Queensland 4072, AustraliaQueensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, AustraliaQueensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, AustraliaQueensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland 4101, AustraliaBombyx mori silk fibroin membranes provide a potential delivery vehicle for both cells and extracellular matrix (ECM) components into diseased or injured tissues. We have previously demonstrated the feasibility of growing retinal pigment epithelial cells (RPE) on fibroin membranes with the view to repairing the retina of patients afflicted with age-related macular degeneration (AMD). The goal of the present study was to investigate the feasibility of incorporating the ECM component elastin, in the form of human recombinant tropoelastin, into these same membranes. Two basic strategies were explored: (1) membranes prepared from blended solutions of fibroin and tropoelastin; and (2) layered constructs prepared from sequentially cast solutions of fibroin, tropoelastin, and fibroin. Optimal conditions for RPE attachment were achieved using a tropoelastin-fibroin blend ratio of 10 to 90 parts by weight. Retention of tropoelastin within the blend and layered constructs was confirmed by immunolabelling and Fourier-transform infrared spectroscopy (FTIR). In the layered constructs, the bulk of tropoelastin was apparently absorbed into the initially cast fibroin layer. Blend membranes displayed higher elastic modulus, percentage elongation, and tensile strength (p < 0.01) when compared to the layered constructs. RPE cell response to fibroin membranes was not affected by the presence of tropoelastin. These findings support the potential use of fibroin membranes for the co-delivery of RPE cells and tropoelastin.http://www.mdpi.com/2079-4983/6/3/946Bombyx morisilk fibrointropoelastinBruch’s membraneretinal pigment epitheliumage-related macular degeneration |
| spellingShingle | Audra M. A. Shadforth Shuko Suzuki Raphaelle Alzonne Grant A. Edwards Neil A. Richardson Traian V. Chirila Damien G. Harkin Incorporation of Human Recombinant Tropoelastin into Silk Fibroin Membranes with the View to Repairing Bruch’s Membrane Journal of Functional Biomaterials Bombyx mori silk fibroin tropoelastin Bruch’s membrane retinal pigment epithelium age-related macular degeneration |
| title | Incorporation of Human Recombinant Tropoelastin into Silk Fibroin Membranes with the View to Repairing Bruch’s Membrane |
| title_full | Incorporation of Human Recombinant Tropoelastin into Silk Fibroin Membranes with the View to Repairing Bruch’s Membrane |
| title_fullStr | Incorporation of Human Recombinant Tropoelastin into Silk Fibroin Membranes with the View to Repairing Bruch’s Membrane |
| title_full_unstemmed | Incorporation of Human Recombinant Tropoelastin into Silk Fibroin Membranes with the View to Repairing Bruch’s Membrane |
| title_short | Incorporation of Human Recombinant Tropoelastin into Silk Fibroin Membranes with the View to Repairing Bruch’s Membrane |
| title_sort | incorporation of human recombinant tropoelastin into silk fibroin membranes with the view to repairing bruch s membrane |
| topic | Bombyx mori silk fibroin tropoelastin Bruch’s membrane retinal pigment epithelium age-related macular degeneration |
| url | http://www.mdpi.com/2079-4983/6/3/946 |
| work_keys_str_mv | AT audramashadforth incorporationofhumanrecombinanttropoelastinintosilkfibroinmembraneswiththeviewtorepairingbruchsmembrane AT shukosuzuki incorporationofhumanrecombinanttropoelastinintosilkfibroinmembraneswiththeviewtorepairingbruchsmembrane AT raphaellealzonne incorporationofhumanrecombinanttropoelastinintosilkfibroinmembraneswiththeviewtorepairingbruchsmembrane AT grantaedwards incorporationofhumanrecombinanttropoelastinintosilkfibroinmembraneswiththeviewtorepairingbruchsmembrane AT neilarichardson incorporationofhumanrecombinanttropoelastinintosilkfibroinmembraneswiththeviewtorepairingbruchsmembrane AT traianvchirila incorporationofhumanrecombinanttropoelastinintosilkfibroinmembraneswiththeviewtorepairingbruchsmembrane AT damiengharkin incorporationofhumanrecombinanttropoelastinintosilkfibroinmembraneswiththeviewtorepairingbruchsmembrane |