Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis
We measured circulating endothelial precursor cells (EPCs), activated circulating endothelial cells (aCECs), and mature circulating endothelial cells (mCECs) using four-color multiparametric flow cytometry in the peripheral blood of 84 chronic myeloid leukemia (CML) patients and 65 healthy controls;...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2015-06-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600509&lng=en&tlng=en |
| _version_ | 1811326447420178432 |
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| author | C.R.T. Godoy D. Levy V. Giampaoli D.A.F. Chamone S.P. Bydlowski J. Pereira |
| author_facet | C.R.T. Godoy D. Levy V. Giampaoli D.A.F. Chamone S.P. Bydlowski J. Pereira |
| author_sort | C.R.T. Godoy |
| collection | DOAJ |
| description | We measured circulating endothelial precursor cells (EPCs), activated circulating endothelial cells (aCECs), and mature circulating endothelial cells (mCECs) using four-color multiparametric flow cytometry in the peripheral blood of 84 chronic myeloid leukemia (CML) patients and 65 healthy controls; and vascular endothelial growth factor (VEGF) by quantitative real-time PCR in 50 CML patients and 32 healthy controls. Because of an increase in mCECs, the median percentage of CECs in CML blast crisis (0.0146%) was significantly higher than in healthy subjects (0.0059%, P<0.01) and in the accelerated phase (0.0059%, P=0.01). There were no significant differences in the percentages of CECs in chronic- or active-phase patients and healthy subjects (P>0.05). In addition, VEGF gene expression was significantly higher in all phases of CML: 0.245 in blast crisis, 0.320 in the active phase, and 0.330 in chronic phase patients than it was in healthy subjects (0.145). In conclusion, CML in blast crisis had increased levels of CECs and VEGF gene expression, which may serve as markers of disease progression and may become targets for the management of CML. |
| first_indexed | 2024-04-13T14:50:28Z |
| format | Article |
| id | doaj.art-1b86afe303da46bf82bed94ec3495775 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-13T14:50:28Z |
| publishDate | 2015-06-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-1b86afe303da46bf82bed94ec34957752022-12-22T02:42:37ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2015-06-0148650951410.1590/1414-431X20153646S0100-879X2015000600509Circulating endothelial cells are increased in chronic myeloid leukemia blast crisisC.R.T. GodoyD. LevyV. GiampaoliD.A.F. ChamoneS.P. BydlowskiJ. PereiraWe measured circulating endothelial precursor cells (EPCs), activated circulating endothelial cells (aCECs), and mature circulating endothelial cells (mCECs) using four-color multiparametric flow cytometry in the peripheral blood of 84 chronic myeloid leukemia (CML) patients and 65 healthy controls; and vascular endothelial growth factor (VEGF) by quantitative real-time PCR in 50 CML patients and 32 healthy controls. Because of an increase in mCECs, the median percentage of CECs in CML blast crisis (0.0146%) was significantly higher than in healthy subjects (0.0059%, P<0.01) and in the accelerated phase (0.0059%, P=0.01). There were no significant differences in the percentages of CECs in chronic- or active-phase patients and healthy subjects (P>0.05). In addition, VEGF gene expression was significantly higher in all phases of CML: 0.245 in blast crisis, 0.320 in the active phase, and 0.330 in chronic phase patients than it was in healthy subjects (0.145). In conclusion, CML in blast crisis had increased levels of CECs and VEGF gene expression, which may serve as markers of disease progression and may become targets for the management of CML.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600509&lng=en&tlng=enCirculating endothelial cellsVascular endothelial growth factorChronic myeloid leukemiaFlow cytometryAngiogenesis |
| spellingShingle | C.R.T. Godoy D. Levy V. Giampaoli D.A.F. Chamone S.P. Bydlowski J. Pereira Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis Brazilian Journal of Medical and Biological Research Circulating endothelial cells Vascular endothelial growth factor Chronic myeloid leukemia Flow cytometry Angiogenesis |
| title | Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis |
| title_full | Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis |
| title_fullStr | Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis |
| title_full_unstemmed | Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis |
| title_short | Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis |
| title_sort | circulating endothelial cells are increased in chronic myeloid leukemia blast crisis |
| topic | Circulating endothelial cells Vascular endothelial growth factor Chronic myeloid leukemia Flow cytometry Angiogenesis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600509&lng=en&tlng=en |
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