HPV and p16 expression association with 5-year survival in oral squamous cell carcinoma patients of north-east India
Background: In our study, we examined the 5-year survival of OSCC patients with HPV positive or negative status along with p16 protein expression. Method: A total of 72 biopsy tissue specimens from histologically confirmed oral squamous cell carcinoma (OSCC) patients were collected. HPV detection an...
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| Language: | English |
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Elsevier
2024-07-01
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| Series: | Advances in Cancer Biology - Metastasis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667394024000029 |
| _version_ | 1797302455484022784 |
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| author | Rajjyoti Das Rupesh Kumar Avdhesh Kumar Rai Anupam Sarma Lopamudra Kakoti Amal Chandra Kataki Mouchumee Bhattacharyya Manoj Kalita |
| author_facet | Rajjyoti Das Rupesh Kumar Avdhesh Kumar Rai Anupam Sarma Lopamudra Kakoti Amal Chandra Kataki Mouchumee Bhattacharyya Manoj Kalita |
| author_sort | Rajjyoti Das |
| collection | DOAJ |
| description | Background: In our study, we examined the 5-year survival of OSCC patients with HPV positive or negative status along with p16 protein expression. Method: A total of 72 biopsy tissue specimens from histologically confirmed oral squamous cell carcinoma (OSCC) patients were collected. HPV detection and genotyping were performed using HPV E6/E7 and HPV- type-specific multiplex primer for nested-PCR. Immunohistochemistry evaluation of pl6 was conducted. SPSS statistical software (ver 20) was used for data analysis. Results: High risk-HPV (hr-HPV) DNA positivity was found in 27.7% (n = 20) of OSCC patients. Stage III OSCC patients were 7.80 times more likely to survive 5 years than stage IV patients (OR-7.80 CI-95%; P-0.03). Among the hr-HPV positive OSCC patients, we found that the median survival time for the 1st year (95%), 3 years (78.5%), and 5 years (38.5%) was significantly higher than that of the hr-HPV negative [1st year (78.6%), 3 years (45.2%) and 5 years (38.5%)] OSCC patients (P-0.03 The survival of male patients with hr-HPV positive OSCC is 9.75 times greater than the survival of patients with HPV negative OSCC (OR-9.75; CI-95%; P-0.05). The p16 expression level (low to overexpression) group and negative P16 expression group of OSCC patients have not demonstrated a significant association with 5-year survival. Conclusion: We conclude that in OSCC cases of North-East India, the presence of hr-HPV in OSCC cases could be a good predictor of 5-year survival rate. Expression of p16 does not appear to have any significant association with 5-year survival. |
| first_indexed | 2024-03-07T23:38:03Z |
| format | Article |
| id | doaj.art-1b87ef8757bc4e598a54a66aad347b8e |
| institution | Directory Open Access Journal |
| issn | 2667-3940 |
| language | English |
| last_indexed | 2024-03-07T23:38:03Z |
| publishDate | 2024-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Cancer Biology - Metastasis |
| spelling | doaj.art-1b87ef8757bc4e598a54a66aad347b8e2024-02-20T04:19:58ZengElsevierAdvances in Cancer Biology - Metastasis2667-39402024-07-0110100115HPV and p16 expression association with 5-year survival in oral squamous cell carcinoma patients of north-east IndiaRajjyoti Das0Rupesh Kumar1Avdhesh Kumar Rai2Anupam Sarma3Lopamudra Kakoti4Amal Chandra Kataki5Mouchumee Bhattacharyya6Manoj Kalita7Dr. B. Borooah Cancer Institute, Department of Head & Neck Surgery, Guwahati, 781016, Assam, IndiaDepartment of Biotechnology, The Assam Royal Global University, Guwahati, 781035, Assam, India; Department of Medical Laboratory Technology, The Assam Royal Global University, Guwahati, 781035, Assam, India; Corresponding author. Department of Bio-Technology, Royal, Global University, Guwahati, 781035, Assam, India.Dr. B. Borooah Cancer Institute, DBT Centre for Molecular Biology & Cancer Research, Guwahati, 781016, Assam, India; Corresponding author. DBT Centre for Molecular Biology & Cancer Research, Dr. B. Borooah Cancer Institute, A.K.Azad Road, Gopinath Nagar, Guwahati, 781016, Assam, India.Dr. B. Borooah Cancer Institute, Department of Oncopathology, Guwahati, 781016, Assam, IndiaDr. B. Borooah Cancer Institute, Department of Oncopathology, Guwahati, 781016, Assam, IndiaDr B. Borooah Cancer Institute, Former Director, Guwahati, 781016, Assam, IndiaDr B. Borooah Cancer Institute, Department of Radiation Oncology, Guwahati, 781016, Assam, IndiaDr B. Borooah Cancer Institute, Population Based Cancer Registry, Guwahati, 781016, Assam, IndiaBackground: In our study, we examined the 5-year survival of OSCC patients with HPV positive or negative status along with p16 protein expression. Method: A total of 72 biopsy tissue specimens from histologically confirmed oral squamous cell carcinoma (OSCC) patients were collected. HPV detection and genotyping were performed using HPV E6/E7 and HPV- type-specific multiplex primer for nested-PCR. Immunohistochemistry evaluation of pl6 was conducted. SPSS statistical software (ver 20) was used for data analysis. Results: High risk-HPV (hr-HPV) DNA positivity was found in 27.7% (n = 20) of OSCC patients. Stage III OSCC patients were 7.80 times more likely to survive 5 years than stage IV patients (OR-7.80 CI-95%; P-0.03). Among the hr-HPV positive OSCC patients, we found that the median survival time for the 1st year (95%), 3 years (78.5%), and 5 years (38.5%) was significantly higher than that of the hr-HPV negative [1st year (78.6%), 3 years (45.2%) and 5 years (38.5%)] OSCC patients (P-0.03 The survival of male patients with hr-HPV positive OSCC is 9.75 times greater than the survival of patients with HPV negative OSCC (OR-9.75; CI-95%; P-0.05). The p16 expression level (low to overexpression) group and negative P16 expression group of OSCC patients have not demonstrated a significant association with 5-year survival. Conclusion: We conclude that in OSCC cases of North-East India, the presence of hr-HPV in OSCC cases could be a good predictor of 5-year survival rate. Expression of p16 does not appear to have any significant association with 5-year survival.http://www.sciencedirect.com/science/article/pii/S2667394024000029Oral cancerp16Human papiloma virusPCRImmunohistochemistrySurvival |
| spellingShingle | Rajjyoti Das Rupesh Kumar Avdhesh Kumar Rai Anupam Sarma Lopamudra Kakoti Amal Chandra Kataki Mouchumee Bhattacharyya Manoj Kalita HPV and p16 expression association with 5-year survival in oral squamous cell carcinoma patients of north-east India Advances in Cancer Biology - Metastasis Oral cancer p16 Human papiloma virus PCR Immunohistochemistry Survival |
| title | HPV and p16 expression association with 5-year survival in oral squamous cell carcinoma patients of north-east India |
| title_full | HPV and p16 expression association with 5-year survival in oral squamous cell carcinoma patients of north-east India |
| title_fullStr | HPV and p16 expression association with 5-year survival in oral squamous cell carcinoma patients of north-east India |
| title_full_unstemmed | HPV and p16 expression association with 5-year survival in oral squamous cell carcinoma patients of north-east India |
| title_short | HPV and p16 expression association with 5-year survival in oral squamous cell carcinoma patients of north-east India |
| title_sort | hpv and p16 expression association with 5 year survival in oral squamous cell carcinoma patients of north east india |
| topic | Oral cancer p16 Human papiloma virus PCR Immunohistochemistry Survival |
| url | http://www.sciencedirect.com/science/article/pii/S2667394024000029 |
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