A Study of the Molecular Regulatory Network of <i>VcTCP18</i> during Blueberry Bud Dormancy
<i>BRANCHED1</i> (<i>BRC1</i>) is a crucial member of the <i>TEOSINTE BRANCHED1/CYCLOIDEA/PCF</i> (TCP) gene family and is well known for playing a central role in shoot branching by controlling buds’ paradormancy. However, the expression characteristics and molec...
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MDPI AG
2023-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/14/2595 |
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| author | Ruixue Li Rui Ma Yuling Zheng Qi Zhao Yu Zong Youyin Zhu Wenrong Chen Yongqiang Li Weidong Guo |
| author_facet | Ruixue Li Rui Ma Yuling Zheng Qi Zhao Yu Zong Youyin Zhu Wenrong Chen Yongqiang Li Weidong Guo |
| author_sort | Ruixue Li |
| collection | DOAJ |
| description | <i>BRANCHED1</i> (<i>BRC1</i>) is a crucial member of the <i>TEOSINTE BRANCHED1/CYCLOIDEA/PCF</i> (TCP) gene family and is well known for playing a central role in shoot branching by controlling buds’ paradormancy. However, the expression characteristics and molecular regulatory mechanism of <i>BRC1</i> during blueberry bud dormancy are unclear. To shed light on these topics, shoots of three blueberry cultivars with different chilling requirements (CRs) were decapitated in summer to induce paradormancy release and subjected to different levels of chilling in winter to induce endodormancy release. The results showed that the high-CR cultivar ‘Chandler’ had the strongest apical dominance among the three cultivars; additionally, the expression of <i>VcTCP18</i>, which is homologous to <i>BRC1</i>, was the highest under both the decapitation treatment and low-temperature treatment. The ‘Emerald’ cultivar, with a low CR, demonstrated the opposite trend. These findings suggest that <i>VcTCP18</i> plays a negative regulatory role in bud break and that there may be a correlation between the CR and tree shape. Through yeast 1-hybrid (Y1H) assays, we finally screened 21 upstream regulatory genes, including eight transcription factors: zinc-finger homeodomain protein 1/4/5/9, MYB4, AP2-like ethylene-responsive transcription factor AINTEGUMENTA (ANT), ASIL2-like, and bHLH035. It was found that these upstream regulatory genes positively or negatively regulated the expression of <i>VcTCP18</i> based on the transcriptome expression profile. In summary, this study enriched our understanding of the regulatory network of <i>BRCl</i> during bud dormancy and provided new insights into the function of <i>BRC1</i>. |
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| language | English |
| last_indexed | 2024-03-11T00:42:41Z |
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| series | Plants |
| spelling | doaj.art-1b898662ec114c42b05f153ff73a08d02023-11-18T20:59:17ZengMDPI AGPlants2223-77472023-07-011214259510.3390/plants12142595A Study of the Molecular Regulatory Network of <i>VcTCP18</i> during Blueberry Bud DormancyRuixue Li0Rui Ma1Yuling Zheng2Qi Zhao3Yu Zong4Youyin Zhu5Wenrong Chen6Yongqiang Li7Weidong Guo8College of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaSchool of Agricultural, Jinhua Polytechinc, Jinhua 321007, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, ChinaCollege of Life Sciences, Zhejiang Normal University, Jinhua 321004, China<i>BRANCHED1</i> (<i>BRC1</i>) is a crucial member of the <i>TEOSINTE BRANCHED1/CYCLOIDEA/PCF</i> (TCP) gene family and is well known for playing a central role in shoot branching by controlling buds’ paradormancy. However, the expression characteristics and molecular regulatory mechanism of <i>BRC1</i> during blueberry bud dormancy are unclear. To shed light on these topics, shoots of three blueberry cultivars with different chilling requirements (CRs) were decapitated in summer to induce paradormancy release and subjected to different levels of chilling in winter to induce endodormancy release. The results showed that the high-CR cultivar ‘Chandler’ had the strongest apical dominance among the three cultivars; additionally, the expression of <i>VcTCP18</i>, which is homologous to <i>BRC1</i>, was the highest under both the decapitation treatment and low-temperature treatment. The ‘Emerald’ cultivar, with a low CR, demonstrated the opposite trend. These findings suggest that <i>VcTCP18</i> plays a negative regulatory role in bud break and that there may be a correlation between the CR and tree shape. Through yeast 1-hybrid (Y1H) assays, we finally screened 21 upstream regulatory genes, including eight transcription factors: zinc-finger homeodomain protein 1/4/5/9, MYB4, AP2-like ethylene-responsive transcription factor AINTEGUMENTA (ANT), ASIL2-like, and bHLH035. It was found that these upstream regulatory genes positively or negatively regulated the expression of <i>VcTCP18</i> based on the transcriptome expression profile. In summary, this study enriched our understanding of the regulatory network of <i>BRCl</i> during bud dormancy and provided new insights into the function of <i>BRC1</i>.https://www.mdpi.com/2223-7747/12/14/2595blueberry<i>VcTCP18</i><i>BRC1</i>yeast one-hybridupstream regulatory genebud dormancy |
| spellingShingle | Ruixue Li Rui Ma Yuling Zheng Qi Zhao Yu Zong Youyin Zhu Wenrong Chen Yongqiang Li Weidong Guo A Study of the Molecular Regulatory Network of <i>VcTCP18</i> during Blueberry Bud Dormancy Plants blueberry <i>VcTCP18</i> <i>BRC1</i> yeast one-hybrid upstream regulatory gene bud dormancy |
| title | A Study of the Molecular Regulatory Network of <i>VcTCP18</i> during Blueberry Bud Dormancy |
| title_full | A Study of the Molecular Regulatory Network of <i>VcTCP18</i> during Blueberry Bud Dormancy |
| title_fullStr | A Study of the Molecular Regulatory Network of <i>VcTCP18</i> during Blueberry Bud Dormancy |
| title_full_unstemmed | A Study of the Molecular Regulatory Network of <i>VcTCP18</i> during Blueberry Bud Dormancy |
| title_short | A Study of the Molecular Regulatory Network of <i>VcTCP18</i> during Blueberry Bud Dormancy |
| title_sort | study of the molecular regulatory network of i vctcp18 i during blueberry bud dormancy |
| topic | blueberry <i>VcTCP18</i> <i>BRC1</i> yeast one-hybrid upstream regulatory gene bud dormancy |
| url | https://www.mdpi.com/2223-7747/12/14/2595 |
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