Malignant Ascites Promote Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium and Fibroblasts
Although malignant ascites (MAs) are known to contribute to various aspects of ovarian cancer progression, knowledge regarding their role in the adhesion of cancer cells to normal peritoneal cells is incomplete. Here, we compared the effect of MAs and benign ascites (BAs) on the adhesion of A2780 an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/8/4222 |
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| author | Paweł Uruski Justyna Mikuła-Pietrasik Martyna Pakuła Sylwia Budkiewicz Marcin Drzewiecki Andrey N. Gaiday Małgorzata Wierzowiecka Eryk Naumowicz Rafał Moszyński Andrzej Tykarski Krzysztof Książek |
| author_facet | Paweł Uruski Justyna Mikuła-Pietrasik Martyna Pakuła Sylwia Budkiewicz Marcin Drzewiecki Andrey N. Gaiday Małgorzata Wierzowiecka Eryk Naumowicz Rafał Moszyński Andrzej Tykarski Krzysztof Książek |
| author_sort | Paweł Uruski |
| collection | DOAJ |
| description | Although malignant ascites (MAs) are known to contribute to various aspects of ovarian cancer progression, knowledge regarding their role in the adhesion of cancer cells to normal peritoneal cells is incomplete. Here, we compared the effect of MAs and benign ascites (BAs) on the adhesion of A2780 and OVCAR-3 cancer cells to omentum-derived peritoneal mesothelial cells (PMCs) and peritoneal fibroblasts (PFBs). The results showed that MAs stimulated the adhesion of A2780 and OVCAR-3 cells to PMCs and PFBs more efficiently than did BAs, and the strongest binding occurred when both cancer and normal cells were exposed to the fluid. Intervention studies showed that MAs-driven adhesion of A2780 cells to PMCs/PFBs depends on the presence of TGF-β1 and HGF, whereas binding of OVCAR-3 cells was mediated by TGF-β1, GRO-1, and IGF-1. Moreover, MAs upregulated α5β1 integrin expression on PFBs but not on PMCs or cancer cells, vimentin expression in all cells tested, and ICAM-1 only in cancer cells. When integrin-linked kinase was neutralized in PMCs or PFBs, cancer cell adhesion to PMCs and PFBs decreased. Collectively, our report shows that MAs may contribute to the early stages of ovarian cancer metastasis by modulating the proadhesive interplay between normal and cancer cells. |
| first_indexed | 2024-03-10T12:11:14Z |
| format | Article |
| id | doaj.art-1b89f7f2dd4147c9a08a7f438c58a4c4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:11:14Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1b89f7f2dd4147c9a08a7f438c58a4c42023-11-21T16:12:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01228422210.3390/ijms22084222Malignant Ascites Promote Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium and FibroblastsPaweł Uruski0Justyna Mikuła-Pietrasik1Martyna Pakuła2Sylwia Budkiewicz3Marcin Drzewiecki4Andrey N. Gaiday5Małgorzata Wierzowiecka6Eryk Naumowicz7Rafał Moszyński8Andrzej Tykarski9Krzysztof Książek10Department of Hypertensiology, Poznan University of Medical Sciences, Długa 1/2 Str., 61-848 Poznan, PolandDepartment of Pathophysiology of Ageing and Civilization Diseases, Poznan University of Medical Sciences, Długa 1/2 Str., 61-848 Poznan, PolandDepartment of Hypertensiology, Poznan University of Medical Sciences, Długa 1/2 Str., 61-848 Poznan, PolandDepartment of Pathophysiology of Ageing and Civilization Diseases, Poznan University of Medical Sciences, Długa 1/2 Str., 61-848 Poznan, PolandDepartment of Pathophysiology of Ageing and Civilization Diseases, Poznan University of Medical Sciences, Długa 1/2 Str., 61-848 Poznan, PolandDepartment of Obstetrics and Gynecology, West Kazakhstan Marat Ospanov Medical University, 50B 12th Microdistrict Apt.21, Aktobe 030008, KazakhstanDepartment of Hypertensiology, Poznan University of Medical Sciences, Długa 1/2 Str., 61-848 Poznan, PolandGeneral Surgery Ward, Medical Centre HCP, 28 Czerwca 1956 r. 223/229 Str., 61-485 Poznan, PolandDivision of Gynecological Surgery, Poznan University of Medical Sciences, Polna 33 Str., 60-535 Poznan, PolandDepartment of Hypertensiology, Poznan University of Medical Sciences, Długa 1/2 Str., 61-848 Poznan, PolandDepartment of Pathophysiology of Ageing and Civilization Diseases, Poznan University of Medical Sciences, Długa 1/2 Str., 61-848 Poznan, PolandAlthough malignant ascites (MAs) are known to contribute to various aspects of ovarian cancer progression, knowledge regarding their role in the adhesion of cancer cells to normal peritoneal cells is incomplete. Here, we compared the effect of MAs and benign ascites (BAs) on the adhesion of A2780 and OVCAR-3 cancer cells to omentum-derived peritoneal mesothelial cells (PMCs) and peritoneal fibroblasts (PFBs). The results showed that MAs stimulated the adhesion of A2780 and OVCAR-3 cells to PMCs and PFBs more efficiently than did BAs, and the strongest binding occurred when both cancer and normal cells were exposed to the fluid. Intervention studies showed that MAs-driven adhesion of A2780 cells to PMCs/PFBs depends on the presence of TGF-β1 and HGF, whereas binding of OVCAR-3 cells was mediated by TGF-β1, GRO-1, and IGF-1. Moreover, MAs upregulated α5β1 integrin expression on PFBs but not on PMCs or cancer cells, vimentin expression in all cells tested, and ICAM-1 only in cancer cells. When integrin-linked kinase was neutralized in PMCs or PFBs, cancer cell adhesion to PMCs and PFBs decreased. Collectively, our report shows that MAs may contribute to the early stages of ovarian cancer metastasis by modulating the proadhesive interplay between normal and cancer cells.https://www.mdpi.com/1422-0067/22/8/4222cancer cell adhesionmalignant ascitesovarian cancerperitoneal metastases |
| spellingShingle | Paweł Uruski Justyna Mikuła-Pietrasik Martyna Pakuła Sylwia Budkiewicz Marcin Drzewiecki Andrey N. Gaiday Małgorzata Wierzowiecka Eryk Naumowicz Rafał Moszyński Andrzej Tykarski Krzysztof Książek Malignant Ascites Promote Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium and Fibroblasts International Journal of Molecular Sciences cancer cell adhesion malignant ascites ovarian cancer peritoneal metastases |
| title | Malignant Ascites Promote Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium and Fibroblasts |
| title_full | Malignant Ascites Promote Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium and Fibroblasts |
| title_fullStr | Malignant Ascites Promote Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium and Fibroblasts |
| title_full_unstemmed | Malignant Ascites Promote Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium and Fibroblasts |
| title_short | Malignant Ascites Promote Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium and Fibroblasts |
| title_sort | malignant ascites promote adhesion of ovarian cancer cells to peritoneal mesothelium and fibroblasts |
| topic | cancer cell adhesion malignant ascites ovarian cancer peritoneal metastases |
| url | https://www.mdpi.com/1422-0067/22/8/4222 |
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