Three-Dimensional Ex Vivo Culture for Drug Responses of Patient-Derived Gastric Cancer Tissue
Gastric cancer (GC) is one of the most common malignancies with high mortality and substantial morbidity. Although the traditional treatment strategies for GC revolve around surgery, radiotherapy, and chemotherapy, none have been able to optimally treat most affected patients. To improve clinical ou...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-02-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2020.614096/full |
| _version_ | 1828242440584167424 |
|---|---|
| author | Sian Chen Sian Chen Chenbin Chen Chenbin Chen Yuanbo Hu Yuanbo Hu Ce Zhu Xiaozhi Luo Xiaozhi Luo Lizhu Wang Xiang Wang Xiangwei Sun Xiangwei Sun Xiaodong Chen Xiaodong Chen Wangkai Xie Wangkai Xie Han Lou Xielin Huang Chao Li Jun Xu Xiangyang Xue Xian Shen |
| author_facet | Sian Chen Sian Chen Chenbin Chen Chenbin Chen Yuanbo Hu Yuanbo Hu Ce Zhu Xiaozhi Luo Xiaozhi Luo Lizhu Wang Xiang Wang Xiangwei Sun Xiangwei Sun Xiaodong Chen Xiaodong Chen Wangkai Xie Wangkai Xie Han Lou Xielin Huang Chao Li Jun Xu Xiangyang Xue Xian Shen |
| author_sort | Sian Chen |
| collection | DOAJ |
| description | Gastric cancer (GC) is one of the most common malignancies with high mortality and substantial morbidity. Although the traditional treatment strategies for GC revolve around surgery, radiotherapy, and chemotherapy, none have been able to optimally treat most affected patients. To improve clinical outcomes and overcome potential GC resistance, we established a three-dimensional (3D) culturing platform that accurately predicts drug responses in a time- and cost-effective manner. We collected tumor tissues from patients following surgeries and cultured them for 3 days using our protocol. We first evaluated cell proliferation, viability, and apoptosis using the following markers: Ki67 and cleaved caspase 3 (Cas3). We demonstrated that cell viability was maintained for 72 h in culture and that the tumor microenvironments and vascular integrities of the tissues were intact throughout the culture period. We then administered chemotherapeutics to assess drug responses and found differential sensitivity across different patient-derived tissues, enabling us to determine individualized medication plans. Overall, our study validated this rapid, cost-effective, scalable, and reproducible protocol for GC tissue culture that can be employed for drug response assessments. Our 3D culture platform paves a new way for personalized medication in GC and other tumors and can greatly impact future oncological research. |
| first_indexed | 2024-04-12T22:14:53Z |
| format | Article |
| id | doaj.art-1b8e0b560770400d8d3899bdb5d304c4 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-12T22:14:53Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-1b8e0b560770400d8d3899bdb5d304c42022-12-22T03:14:33ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-02-011010.3389/fonc.2020.614096614096Three-Dimensional Ex Vivo Culture for Drug Responses of Patient-Derived Gastric Cancer TissueSian Chen0Sian Chen1Chenbin Chen2Chenbin Chen3Yuanbo Hu4Yuanbo Hu5Ce Zhu6Xiaozhi Luo7Xiaozhi Luo8Lizhu Wang9Xiang Wang10Xiangwei Sun11Xiangwei Sun12Xiaodong Chen13Xiaodong Chen14Wangkai Xie15Wangkai Xie16Han Lou17Xielin Huang18Chao Li19Jun Xu20Xiangyang Xue21Xian Shen22Department of Gastrointestinal Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Pathology, The Second Affiliated Hospital & Yuying Children’s, Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaThe First School of Medicine, Wenzhou Medical University, Wenzhou, ChinaDepartment of Microbiology and Immunology, School of Basic Medical Sciences, Institute of Molecular Virology and Immunology, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaGastric cancer (GC) is one of the most common malignancies with high mortality and substantial morbidity. Although the traditional treatment strategies for GC revolve around surgery, radiotherapy, and chemotherapy, none have been able to optimally treat most affected patients. To improve clinical outcomes and overcome potential GC resistance, we established a three-dimensional (3D) culturing platform that accurately predicts drug responses in a time- and cost-effective manner. We collected tumor tissues from patients following surgeries and cultured them for 3 days using our protocol. We first evaluated cell proliferation, viability, and apoptosis using the following markers: Ki67 and cleaved caspase 3 (Cas3). We demonstrated that cell viability was maintained for 72 h in culture and that the tumor microenvironments and vascular integrities of the tissues were intact throughout the culture period. We then administered chemotherapeutics to assess drug responses and found differential sensitivity across different patient-derived tissues, enabling us to determine individualized medication plans. Overall, our study validated this rapid, cost-effective, scalable, and reproducible protocol for GC tissue culture that can be employed for drug response assessments. Our 3D culture platform paves a new way for personalized medication in GC and other tumors and can greatly impact future oncological research.https://www.frontiersin.org/articles/10.3389/fonc.2020.614096/fullgastric cancerex vivo tumor tissue cultureproliferationapoptosischemotherapy effectiveness |
| spellingShingle | Sian Chen Sian Chen Chenbin Chen Chenbin Chen Yuanbo Hu Yuanbo Hu Ce Zhu Xiaozhi Luo Xiaozhi Luo Lizhu Wang Xiang Wang Xiangwei Sun Xiangwei Sun Xiaodong Chen Xiaodong Chen Wangkai Xie Wangkai Xie Han Lou Xielin Huang Chao Li Jun Xu Xiangyang Xue Xian Shen Three-Dimensional Ex Vivo Culture for Drug Responses of Patient-Derived Gastric Cancer Tissue Frontiers in Oncology gastric cancer ex vivo tumor tissue culture proliferation apoptosis chemotherapy effectiveness |
| title | Three-Dimensional Ex Vivo Culture for Drug Responses of Patient-Derived Gastric Cancer Tissue |
| title_full | Three-Dimensional Ex Vivo Culture for Drug Responses of Patient-Derived Gastric Cancer Tissue |
| title_fullStr | Three-Dimensional Ex Vivo Culture for Drug Responses of Patient-Derived Gastric Cancer Tissue |
| title_full_unstemmed | Three-Dimensional Ex Vivo Culture for Drug Responses of Patient-Derived Gastric Cancer Tissue |
| title_short | Three-Dimensional Ex Vivo Culture for Drug Responses of Patient-Derived Gastric Cancer Tissue |
| title_sort | three dimensional ex vivo culture for drug responses of patient derived gastric cancer tissue |
| topic | gastric cancer ex vivo tumor tissue culture proliferation apoptosis chemotherapy effectiveness |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2020.614096/full |
| work_keys_str_mv | AT sianchen threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT sianchen threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT chenbinchen threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT chenbinchen threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT yuanbohu threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT yuanbohu threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT cezhu threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT xiaozhiluo threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT xiaozhiluo threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT lizhuwang threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT xiangwang threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT xiangweisun threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT xiangweisun threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT xiaodongchen threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT xiaodongchen threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT wangkaixie threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT wangkaixie threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT hanlou threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT xielinhuang threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT chaoli threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT junxu threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT xiangyangxue threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue AT xianshen threedimensionalexvivoculturefordrugresponsesofpatientderivedgastriccancertissue |