Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activation
Abstract Background Ganoderma sp., such as Ganoderma tsugae (GT), play an important role in traditional Chinese medicine. Ganoderma sp. contains several constituents, including Sacacchin, which has recently drawn attention because it can not only enhance the repair of muscle damage but also strength...
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | Chinese Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s13020-020-00381-3 |
| _version_ | 1818503934420975616 |
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| author | Meng-Huang Wu Chuang-Yu Lin Chun-Yin Hou Ming-Thau Sheu Hsi Chang |
| author_facet | Meng-Huang Wu Chuang-Yu Lin Chun-Yin Hou Ming-Thau Sheu Hsi Chang |
| author_sort | Meng-Huang Wu |
| collection | DOAJ |
| description | Abstract Background Ganoderma sp., such as Ganoderma tsugae (GT), play an important role in traditional Chinese medicine. Ganoderma sp. contains several constituents, including Sacacchin, which has recently drawn attention because it can not only enhance the repair of muscle damage but also strengthen the muscle enforcement. Although Ganoderma sp. have a therapeutic effect for neuromuscular disorders, the underlying mechanism remains unclear. This study investigated the effect and underlying molecular mechanism of micronized sacchachitin (mSC) on satellite cells (SCs), which are known as the muscle stem cells. Methods The myogenic cells, included SCs (Pax7+) were isolated from tibialis anterior muscles of a healthy rat and were cultured in growth media with different mSC concentrations. For the evaluation of SC proliferation, these cultivated cells were immunostained with Pax7 and bromodeoxyuridine assessed simultaneously. The molecular signal pathway was further investigated by using Western blotting and signal pathway inhibitors. Results Our data revealed that 200 µg/mL mSC had an optimal capability to significantly enhance the SC proliferation. Furthermore, this enhancement of SC proliferation was verified to be involved with activation of TAK1-JNK-AP-1 signaling pathway through TLR2, whose expression on SC surface was confirmed for the first time here. Conclusion Micronized sacchachitin extracted from GT was capable of promoting the proliferation of SC under a correct concentration. |
| first_indexed | 2024-12-10T21:30:26Z |
| format | Article |
| id | doaj.art-1b8e8f2ad4f54a43ae447f5df06abf5e |
| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-12-10T21:30:26Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj.art-1b8e8f2ad4f54a43ae447f5df06abf5e2022-12-22T01:32:49ZengBMCChinese Medicine1749-85462020-09-0115111210.1186/s13020-020-00381-3Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activationMeng-Huang Wu0Chuang-Yu Lin1Chun-Yin Hou2Ming-Thau Sheu3Hsi Chang4Department of Orthopedics, Taipei Medical University HospitalDepartment of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto UniversityDepartment of Family Medicine, Taipei City HospitalSchool of Pharmacy, College of Pharmacy, Taipei Medical UniversityDepartment of Pediatrics, School of Medicine, College of Medicine, Taipei Medical UniversityAbstract Background Ganoderma sp., such as Ganoderma tsugae (GT), play an important role in traditional Chinese medicine. Ganoderma sp. contains several constituents, including Sacacchin, which has recently drawn attention because it can not only enhance the repair of muscle damage but also strengthen the muscle enforcement. Although Ganoderma sp. have a therapeutic effect for neuromuscular disorders, the underlying mechanism remains unclear. This study investigated the effect and underlying molecular mechanism of micronized sacchachitin (mSC) on satellite cells (SCs), which are known as the muscle stem cells. Methods The myogenic cells, included SCs (Pax7+) were isolated from tibialis anterior muscles of a healthy rat and were cultured in growth media with different mSC concentrations. For the evaluation of SC proliferation, these cultivated cells were immunostained with Pax7 and bromodeoxyuridine assessed simultaneously. The molecular signal pathway was further investigated by using Western blotting and signal pathway inhibitors. Results Our data revealed that 200 µg/mL mSC had an optimal capability to significantly enhance the SC proliferation. Furthermore, this enhancement of SC proliferation was verified to be involved with activation of TAK1-JNK-AP-1 signaling pathway through TLR2, whose expression on SC surface was confirmed for the first time here. Conclusion Micronized sacchachitin extracted from GT was capable of promoting the proliferation of SC under a correct concentration.http://link.springer.com/article/10.1186/s13020-020-00381-3SacchachitinSatellite cellsTAK1-JNK-AP-1 signaling pathwayMAPK signal pathwayMuscle regeneration |
| spellingShingle | Meng-Huang Wu Chuang-Yu Lin Chun-Yin Hou Ming-Thau Sheu Hsi Chang Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activation Chinese Medicine Sacchachitin Satellite cells TAK1-JNK-AP-1 signaling pathway MAPK signal pathway Muscle regeneration |
| title | Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activation |
| title_full | Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activation |
| title_fullStr | Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activation |
| title_full_unstemmed | Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activation |
| title_short | Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activation |
| title_sort | micronized sacchachitin promotes satellite cell proliferation through tak1 jnk ap 1 signaling pathway predominantly by tlr2 activation |
| topic | Sacchachitin Satellite cells TAK1-JNK-AP-1 signaling pathway MAPK signal pathway Muscle regeneration |
| url | http://link.springer.com/article/10.1186/s13020-020-00381-3 |
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