Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses
Burkholderia cepacia is an opportunistic pathogen that infects patients with debilitating underlying diseases. This study investigated the production of outer membrane vesicles (OMVs) by B. cepacia cultured with sub-minimum inhibitory concentrations (MICs) of antibiotics and examined their pathogeni...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2020.1802193 |
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| author | Se Yeon Kim Mi Hyun Kim Joo Hee Son Seung Il Kim Sung Ho Yun Kyeongmin Kim Shukho Kim Minsang Shin Je Chul Lee |
| author_facet | Se Yeon Kim Mi Hyun Kim Joo Hee Son Seung Il Kim Sung Ho Yun Kyeongmin Kim Shukho Kim Minsang Shin Je Chul Lee |
| author_sort | Se Yeon Kim |
| collection | DOAJ |
| description | Burkholderia cepacia is an opportunistic pathogen that infects patients with debilitating underlying diseases. This study investigated the production of outer membrane vesicles (OMVs) by B. cepacia cultured with sub-minimum inhibitory concentrations (MICs) of antibiotics and examined their pathogenic roles both in vitro and in vivo. B. cepacia ATCC 25416 produced more OMVs under antibiotic stress conditions than controls. OMVs isolated from B. cepacia cultured in Luria-Bertani (LB) broth (OMVs/LB) induced cytotoxicity and the expression of pro-inflammatory cytokine genes in A549 cells in a dose-dependent manner. Host cell cytotoxicity and pro-inflammatory responses were significantly higher in A549 cells treated with B. cepacia OMVs cultured with 1/4 MIC of ceftazidime (OMVs/CAZ) than in the cells treated with OMVs/LB, OMVs cultured with 1/4 MIC of trimethoprim/sulfamethoxazole (OMVs/SXT), or OMVs cultured with 1/4 MIC of meropenem. Intratracheal injection of B. cepacia OMVs also induced histopathology in vivo in mouse lungs. Expressions of IL-1β and TNF-α genes were significantly up-regulatedin the lungs of mice treated with OMVs/CAZ compared to mice administered other OMVs; the expression of the GRO-α gene, however, was significantly up-regulated in OMVs/SXT. In conclusion, OMVs produced by B. cepacia under different antibiotic stress conditions induce different host responses that may contribute to the pathogenesis of B. cepacia. |
| first_indexed | 2024-12-22T01:50:42Z |
| format | Article |
| id | doaj.art-1b9073f84fcf45e48bc1f7bdd1e831ac |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-12-22T01:50:42Z |
| publishDate | 2020-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-1b9073f84fcf45e48bc1f7bdd1e831ac2022-12-21T18:42:56ZengTaylor & Francis GroupVirulence2150-55942150-56082020-12-01111995100510.1080/21505594.2020.18021931802193Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responsesSe Yeon Kim0Mi Hyun Kim1Joo Hee Son2Seung Il Kim3Sung Ho Yun4Kyeongmin Kim5Shukho Kim6Minsang Shin7Je Chul Lee8School of Medicine, Kyungpook National UniversitySchool of Medicine, Kyungpook National UniversitySchool of Medicine, Kyungpook National UniversityKorea Basic Science InstituteKorea Basic Science InstituteSchool of Medicine, Kyungpook National UniversitySchool of Medicine, Kyungpook National UniversitySchool of Medicine, Kyungpook National UniversitySchool of Medicine, Kyungpook National UniversityBurkholderia cepacia is an opportunistic pathogen that infects patients with debilitating underlying diseases. This study investigated the production of outer membrane vesicles (OMVs) by B. cepacia cultured with sub-minimum inhibitory concentrations (MICs) of antibiotics and examined their pathogenic roles both in vitro and in vivo. B. cepacia ATCC 25416 produced more OMVs under antibiotic stress conditions than controls. OMVs isolated from B. cepacia cultured in Luria-Bertani (LB) broth (OMVs/LB) induced cytotoxicity and the expression of pro-inflammatory cytokine genes in A549 cells in a dose-dependent manner. Host cell cytotoxicity and pro-inflammatory responses were significantly higher in A549 cells treated with B. cepacia OMVs cultured with 1/4 MIC of ceftazidime (OMVs/CAZ) than in the cells treated with OMVs/LB, OMVs cultured with 1/4 MIC of trimethoprim/sulfamethoxazole (OMVs/SXT), or OMVs cultured with 1/4 MIC of meropenem. Intratracheal injection of B. cepacia OMVs also induced histopathology in vivo in mouse lungs. Expressions of IL-1β and TNF-α genes were significantly up-regulatedin the lungs of mice treated with OMVs/CAZ compared to mice administered other OMVs; the expression of the GRO-α gene, however, was significantly up-regulated in OMVs/SXT. In conclusion, OMVs produced by B. cepacia under different antibiotic stress conditions induce different host responses that may contribute to the pathogenesis of B. cepacia.http://dx.doi.org/10.1080/21505594.2020.1802193burkholderia cepaciaantibioticsouter membrane vesiclecytotoxicityinflammatory response |
| spellingShingle | Se Yeon Kim Mi Hyun Kim Joo Hee Son Seung Il Kim Sung Ho Yun Kyeongmin Kim Shukho Kim Minsang Shin Je Chul Lee Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses Virulence burkholderia cepacia antibiotics outer membrane vesicle cytotoxicity inflammatory response |
| title | Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses |
| title_full | Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses |
| title_fullStr | Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses |
| title_full_unstemmed | Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses |
| title_short | Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses |
| title_sort | outer membrane vesicles produced by burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro inflammatory responses |
| topic | burkholderia cepacia antibiotics outer membrane vesicle cytotoxicity inflammatory response |
| url | http://dx.doi.org/10.1080/21505594.2020.1802193 |
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