Impiego del sistema Uro-Quick per l’identificazione di resistenze ben caratterizzate agli antibiotici veicolate da diverse specie batteriche

The Uro-Quick system, previously used to carry out antibiotic susceptibility tests directly on urine samples, has been employed for the detection of antibiotic resistance traits that might be not easily detected in well-characterized bacterial species.The period of time requested by the instrument t...

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Main Authors: Simona Roveta, Anna Marchese, Eugenio A. Debbia
Format: Article
Language:English
Published: PAGEPress Publications 2003-03-01
Series:Microbiologia Medica
Subjects:
Online Access:http://www.pagepressjournals.org/index.php/mm/article/view/3059
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author Simona Roveta
Anna Marchese
Eugenio A. Debbia
author_facet Simona Roveta
Anna Marchese
Eugenio A. Debbia
author_sort Simona Roveta
collection DOAJ
description The Uro-Quick system, previously used to carry out antibiotic susceptibility tests directly on urine samples, has been employed for the detection of antibiotic resistance traits that might be not easily detected in well-characterized bacterial species.The period of time requested by the instrument to identify the drug resistance were: penicillin-resistant (PEN-R), constitutive and inducible oxacillin-resistant (OXA-R) Staphylococcus aureus, OXA-R S. epidermidis 480 min.Teicoplanin-resistant (TEI-R) S. haemolyticus, Vancomycin- (VanA,VanB,VanC), aminoglycoside- resistant (HLAR) Enterococcus spp, 24h. S. pyogenes exhibiting the three erythromycin-resistance phenotypes (Ery-R,C, I e M) 480 min.Ampicillin- (AMP-R), ciprofloxacin (CIP-R) and clavulanic acid-resistant (IRT) Escherichia coli 180 min. Imipenem-resistant (IMI-R) Pseudomonas aeruginosa 24h. For each strain was evaluated the optimal experimental conditions including inoculum size, time of incubation and antibiotic concentration. The results obtained indicated that Uro-Quick might be employed for the correct detection of the above antibiotic resistant markers.
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spelling doaj.art-1b914addfc6a46fb9d89c9810401cb9e2022-12-21T23:21:21ZengPAGEPress PublicationsMicrobiologia Medica2280-64232003-03-0118110.4081/mm.2003.30592307Impiego del sistema Uro-Quick per l’identificazione di resistenze ben caratterizzate agli antibiotici veicolate da diverse specie battericheSimona RovetaAnna MarcheseEugenio A. DebbiaThe Uro-Quick system, previously used to carry out antibiotic susceptibility tests directly on urine samples, has been employed for the detection of antibiotic resistance traits that might be not easily detected in well-characterized bacterial species.The period of time requested by the instrument to identify the drug resistance were: penicillin-resistant (PEN-R), constitutive and inducible oxacillin-resistant (OXA-R) Staphylococcus aureus, OXA-R S. epidermidis 480 min.Teicoplanin-resistant (TEI-R) S. haemolyticus, Vancomycin- (VanA,VanB,VanC), aminoglycoside- resistant (HLAR) Enterococcus spp, 24h. S. pyogenes exhibiting the three erythromycin-resistance phenotypes (Ery-R,C, I e M) 480 min.Ampicillin- (AMP-R), ciprofloxacin (CIP-R) and clavulanic acid-resistant (IRT) Escherichia coli 180 min. Imipenem-resistant (IMI-R) Pseudomonas aeruginosa 24h. For each strain was evaluated the optimal experimental conditions including inoculum size, time of incubation and antibiotic concentration. The results obtained indicated that Uro-Quick might be employed for the correct detection of the above antibiotic resistant markers.http://www.pagepressjournals.org/index.php/mm/article/view/3059Uro-Quick, Detection of antibiotic resistance,Well-characterized strains
spellingShingle Simona Roveta
Anna Marchese
Eugenio A. Debbia
Impiego del sistema Uro-Quick per l’identificazione di resistenze ben caratterizzate agli antibiotici veicolate da diverse specie batteriche
Microbiologia Medica
Uro-Quick, Detection of antibiotic resistance,Well-characterized strains
title Impiego del sistema Uro-Quick per l’identificazione di resistenze ben caratterizzate agli antibiotici veicolate da diverse specie batteriche
title_full Impiego del sistema Uro-Quick per l’identificazione di resistenze ben caratterizzate agli antibiotici veicolate da diverse specie batteriche
title_fullStr Impiego del sistema Uro-Quick per l’identificazione di resistenze ben caratterizzate agli antibiotici veicolate da diverse specie batteriche
title_full_unstemmed Impiego del sistema Uro-Quick per l’identificazione di resistenze ben caratterizzate agli antibiotici veicolate da diverse specie batteriche
title_short Impiego del sistema Uro-Quick per l’identificazione di resistenze ben caratterizzate agli antibiotici veicolate da diverse specie batteriche
title_sort impiego del sistema uro quick per l identificazione di resistenze ben caratterizzate agli antibiotici veicolate da diverse specie batteriche
topic Uro-Quick, Detection of antibiotic resistance,Well-characterized strains
url http://www.pagepressjournals.org/index.php/mm/article/view/3059
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