Prevalence of Class D Carbapenemases among Extended-Spectrum β-Lactamases Producing Escherichia coli Isolates from Educational Hospitals in Shahrekord
Introduction: Extended-spectrum β-lactamases (ESBLs) are a set of plasmid-borne, various and quickly evolving enzymes that are a main therapeutic issue now-a-days for inpatient and outpatient treatment. Aim: The aim of this study was to determine multi-drug resistance (MDR) and ESBLs producing...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2016-05-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/7739/17722_CE(RA1)_F(T)_PF1(BMAK)_PFA(AK)_PF2(PAG).pdf |
| _version_ | 1828749737705078784 |
|---|---|
| author | Mohammad-Sadegh Damavandi Abolfazl Gholipour Mohammad Latif Pour |
| author_facet | Mohammad-Sadegh Damavandi Abolfazl Gholipour Mohammad Latif Pour |
| author_sort | Mohammad-Sadegh Damavandi |
| collection | DOAJ |
| description | Introduction: Extended-spectrum β-lactamases (ESBLs) are
a set of plasmid-borne, various and quickly evolving enzymes
that are a main therapeutic issue now-a-days for inpatient and
outpatient treatment.
Aim: The aim of this study was to determine multi-drug
resistance (MDR) and ESBLs producing E. coli strains,
prevalence of class D Carbapenemases among ESBLs
producing Escherichia coli isolates from educational hospitals
in Shahrekord, Iran.
Materials and Methods: Uropathogenic Escherichia coli strains
were isolated from patients with Urinary Tract Infections (UTIs).
The agar disc diffusion test was used to characterize the
antimicrobial sensitivity of the E. coli isolates. The ESBL positive
strains were identified by phenotypic double-disk synergy test,
by third-generation cephalosporin in combination with or without
clavulanic acid. Multiplex PCR was carried out for detection
of the three families of OXA-type carbapenamases including
OXA-23, OXA-24, and OXA-48 in E. coli strains.
Results: All bacterial isolates were susceptible to meropenem.
Ninety isolates produced ESBL, 55 E. coli isolates from inpatients,
and 35 isolates from outpatients, with a significant association
(p< 0.05). The prevalence of OXA-23, OXA-24, and OXA-48 in
the ESBLs producing isolates was respectively 21%, 18%, and
11% for inpatients, and 10%, 8%, and 6% for outpatients.
Conclusion: ESBL-producing E. coli isolates are also a major
threat in the clinical setting. The findings of this study indicated
the high occurrence of ESBLs and multiple antibiotic resistance
in E. coli isolates. |
| first_indexed | 2024-12-10T20:26:38Z |
| format | Article |
| id | doaj.art-1b927a73cab440b4ae5064550eb9c349 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-10T20:26:38Z |
| publishDate | 2016-05-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-1b927a73cab440b4ae5064550eb9c3492022-12-22T01:34:49ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-05-01105DC01DC0510.7860/JCDR/2016/17722.7739Prevalence of Class D Carbapenemases among Extended-Spectrum β-Lactamases Producing Escherichia coli Isolates from Educational Hospitals in ShahrekordMohammad-Sadegh Damavandi0Abolfazl Gholipour1Mohammad Latif Pour2Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran.Assistant Professor, Department of Microbiology and Immunology, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.Student, Department of Microbiology and Immunology, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.Introduction: Extended-spectrum β-lactamases (ESBLs) are a set of plasmid-borne, various and quickly evolving enzymes that are a main therapeutic issue now-a-days for inpatient and outpatient treatment. Aim: The aim of this study was to determine multi-drug resistance (MDR) and ESBLs producing E. coli strains, prevalence of class D Carbapenemases among ESBLs producing Escherichia coli isolates from educational hospitals in Shahrekord, Iran. Materials and Methods: Uropathogenic Escherichia coli strains were isolated from patients with Urinary Tract Infections (UTIs). The agar disc diffusion test was used to characterize the antimicrobial sensitivity of the E. coli isolates. The ESBL positive strains were identified by phenotypic double-disk synergy test, by third-generation cephalosporin in combination with or without clavulanic acid. Multiplex PCR was carried out for detection of the three families of OXA-type carbapenamases including OXA-23, OXA-24, and OXA-48 in E. coli strains. Results: All bacterial isolates were susceptible to meropenem. Ninety isolates produced ESBL, 55 E. coli isolates from inpatients, and 35 isolates from outpatients, with a significant association (p< 0.05). The prevalence of OXA-23, OXA-24, and OXA-48 in the ESBLs producing isolates was respectively 21%, 18%, and 11% for inpatients, and 10%, 8%, and 6% for outpatients. Conclusion: ESBL-producing E. coli isolates are also a major threat in the clinical setting. The findings of this study indicated the high occurrence of ESBLs and multiple antibiotic resistance in E. coli isolates.https://jcdr.net/articles/PDF/7739/17722_CE(RA1)_F(T)_PF1(BMAK)_PFA(AK)_PF2(PAG).pdfbacterial resistancemultiplex polymerase chain reactionurinary tract infections |
| spellingShingle | Mohammad-Sadegh Damavandi Abolfazl Gholipour Mohammad Latif Pour Prevalence of Class D Carbapenemases among Extended-Spectrum β-Lactamases Producing Escherichia coli Isolates from Educational Hospitals in Shahrekord Journal of Clinical and Diagnostic Research bacterial resistance multiplex polymerase chain reaction urinary tract infections |
| title | Prevalence of Class D Carbapenemases among Extended-Spectrum β-Lactamases Producing Escherichia coli Isolates from Educational Hospitals in Shahrekord |
| title_full | Prevalence of Class D Carbapenemases among Extended-Spectrum β-Lactamases Producing Escherichia coli Isolates from Educational Hospitals in Shahrekord |
| title_fullStr | Prevalence of Class D Carbapenemases among Extended-Spectrum β-Lactamases Producing Escherichia coli Isolates from Educational Hospitals in Shahrekord |
| title_full_unstemmed | Prevalence of Class D Carbapenemases among Extended-Spectrum β-Lactamases Producing Escherichia coli Isolates from Educational Hospitals in Shahrekord |
| title_short | Prevalence of Class D Carbapenemases among Extended-Spectrum β-Lactamases Producing Escherichia coli Isolates from Educational Hospitals in Shahrekord |
| title_sort | prevalence of class d carbapenemases among extended spectrum β lactamases producing escherichia coli isolates from educational hospitals in shahrekord |
| topic | bacterial resistance multiplex polymerase chain reaction urinary tract infections |
| url | https://jcdr.net/articles/PDF/7739/17722_CE(RA1)_F(T)_PF1(BMAK)_PFA(AK)_PF2(PAG).pdf |
| work_keys_str_mv | AT mohammadsadeghdamavandi prevalenceofclassdcarbapenemasesamongextendedspectrumblactamasesproducingescherichiacoliisolatesfromeducationalhospitalsinshahrekord AT abolfazlgholipour prevalenceofclassdcarbapenemasesamongextendedspectrumblactamasesproducingescherichiacoliisolatesfromeducationalhospitalsinshahrekord AT mohammadlatifpour prevalenceofclassdcarbapenemasesamongextendedspectrumblactamasesproducingescherichiacoliisolatesfromeducationalhospitalsinshahrekord |