Ex vivo, in situ perfusion protocol for human brain fixation compatible with microscopy, MRI techniques, and anatomical studies

Ex vivo, in situ perfusion protocol for human brain fixation compatible with microscopy, MRI techniques, and anatomical studies

We present a method for human brain fixation based on simultaneous perfusion of 4% paraformaldehyde through carotids after a flush with saline. The left carotid cannula is used to perfuse the body with 10% formalin, to allow further use of the body for anatomical research or teaching. The aim of our...

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Main Authors: Ricardo Insausti, Ana María Insausti, Mónica Muñoz López, Isidro Medina Lorenzo, Maria del Mar Arroyo-Jiménez, María Pilar Marcos Rabal, Carlos de la Rosa-Prieto, José Carlos Delgado-González, Javier Montón Etxeberria, Sandra Cebada-Sánchez, Juan Francisco Raspeño-García, María Mercedes Iñiguez de Onzoño, Francisco Javier Molina Romero, Ruth Benavides-Piccione, Silvia Tapia-González, Laura E. M. Wisse, Sadhana Ravikumar, David A. Wolk, Javier DeFelipe, Paul Yushkevich, Emilio Artacho-Pérula
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-03-01
Series:Frontiers in Neuroanatomy
Subjects:
human brain
fixation
carotid perfusion
histology
electron microscopy
intracellular injection
Online Access:https://www.frontiersin.org/articles/10.3389/fnana.2023.1149674/full

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