Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma
Serine/arginine-rich splicing factor 7 (SRSF7), a known splicing factor, has been revealed to play oncogenic roles in multiple cancers. However, the mechanisms underlying its oncogenic roles have not been well addressed. Here, based on N6-methyladenosine (m6A) co-methylation network analysis across...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
|
| Series: | Genomics, Proteomics & Bioinformatics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672022921002527 |
| _version_ | 1797372024951144448 |
|---|---|
| author | Yixian Cun Sanqi An Haiqing Zheng Jing Lan Wenfang Chen Wanjun Luo Chengguo Yao Xincheng Li Xiang Huang Xiang Sun Zehong Wu Yameng Hu Ziwen Li Shuxia Zhang Geyan Wu Meisongzhu Yang Miaoling Tang Ruyuan Yu Xinyi Liao Guicheng Gao Wei Zhao Jinkai Wang Jun Li |
| author_facet | Yixian Cun Sanqi An Haiqing Zheng Jing Lan Wenfang Chen Wanjun Luo Chengguo Yao Xincheng Li Xiang Huang Xiang Sun Zehong Wu Yameng Hu Ziwen Li Shuxia Zhang Geyan Wu Meisongzhu Yang Miaoling Tang Ruyuan Yu Xinyi Liao Guicheng Gao Wei Zhao Jinkai Wang Jun Li |
| author_sort | Yixian Cun |
| collection | DOAJ |
| description | Serine/arginine-rich splicing factor 7 (SRSF7), a known splicing factor, has been revealed to play oncogenic roles in multiple cancers. However, the mechanisms underlying its oncogenic roles have not been well addressed. Here, based on N6-methyladenosine (m6A) co-methylation network analysis across diverse cell lines, we find that the gene expression of SRSF7 is positively correlated with glioblastoma (GBM) cell-specific m6A methylation. We then indicate that SRSF7 is a novel m6A regulator, which specifically facilitates the m6A methylation near its binding sites on the mRNAs involved in cell proliferation and migration, through recruiting the methyltransferase complex. Moreover, SRSF7 promotes the proliferation and migration of GBM cells largely dependent on the presence of the m6A methyltransferase. The two m6A sites on the mRNA for PDZ-binding kinase (PBK) are regulated by SRSF7 and partially mediate the effects of SRSF7 in GBM cells through recognition by insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2). Together, our discovery reveals a novel role of SRSF7 in regulating m6A and validates the presence and functional importance of temporal- and spatial-specific regulation of m6A mediated by RNA-binding proteins (RBPs). |
| first_indexed | 2024-03-08T18:30:25Z |
| format | Article |
| id | doaj.art-1b9505eaaf9a423e804ab45d9a0d7448 |
| institution | Directory Open Access Journal |
| issn | 1672-0229 |
| language | English |
| last_indexed | 2024-03-08T18:30:25Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Genomics, Proteomics & Bioinformatics |
| spelling | doaj.art-1b9505eaaf9a423e804ab45d9a0d74482023-12-30T04:42:43ZengElsevierGenomics, Proteomics & Bioinformatics1672-02292023-08-01214707728Specific Regulation of m6A by SRSF7 Promotes the Progression of GlioblastomaYixian Cun0Sanqi An1Haiqing Zheng2Jing Lan3Wenfang Chen4Wanjun Luo5Chengguo Yao6Xincheng Li7Xiang Huang8Xiang Sun9Zehong Wu10Yameng Hu11Ziwen Li12Shuxia Zhang13Geyan Wu14Meisongzhu Yang15Miaoling Tang16Ruyuan Yu17Xinyi Liao18Guicheng Gao19Wei Zhao20Jinkai Wang21Jun Li22Department of Rehabilitation Medicine, Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China; Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Medical Informatics, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou 510080, China; Biosafety Level-3 Laboratory, Life Sciences Institute, Guangxi Medical University, Nanning 530020, ChinaDepartment of Rehabilitation Medicine, Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Medical Informatics, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, ChinaCenter for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Rehabilitation Medicine, Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China; Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Medical Informatics, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Medical Informatics, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Medical Informatics, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Rehabilitation Medicine, Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China; Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Rehabilitation Medicine, Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China; Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Rehabilitation Medicine, Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China; Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaState Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Rehabilitation Medicine, Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China; Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaState Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Rehabilitation Medicine, Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China; Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Rehabilitation Medicine, Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China; Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Medical Informatics, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou 510080, ChinaCenter for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou 510080, ChinaDepartment of Medical Informatics, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou 510080, China; RNA Biomedical Institute, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510080, China; Corresponding authors.Department of Rehabilitation Medicine, Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China; Department of Biochemistry, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China; Corresponding authors.Serine/arginine-rich splicing factor 7 (SRSF7), a known splicing factor, has been revealed to play oncogenic roles in multiple cancers. However, the mechanisms underlying its oncogenic roles have not been well addressed. Here, based on N6-methyladenosine (m6A) co-methylation network analysis across diverse cell lines, we find that the gene expression of SRSF7 is positively correlated with glioblastoma (GBM) cell-specific m6A methylation. We then indicate that SRSF7 is a novel m6A regulator, which specifically facilitates the m6A methylation near its binding sites on the mRNAs involved in cell proliferation and migration, through recruiting the methyltransferase complex. Moreover, SRSF7 promotes the proliferation and migration of GBM cells largely dependent on the presence of the m6A methyltransferase. The two m6A sites on the mRNA for PDZ-binding kinase (PBK) are regulated by SRSF7 and partially mediate the effects of SRSF7 in GBM cells through recognition by insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2). Together, our discovery reveals a novel role of SRSF7 in regulating m6A and validates the presence and functional importance of temporal- and spatial-specific regulation of m6A mediated by RNA-binding proteins (RBPs).http://www.sciencedirect.com/science/article/pii/S1672022921002527m6ASerine/arginine-rich splicing factor 7Cell-specific regulationGlioblastomaPDZ-binding kinase |
| spellingShingle | Yixian Cun Sanqi An Haiqing Zheng Jing Lan Wenfang Chen Wanjun Luo Chengguo Yao Xincheng Li Xiang Huang Xiang Sun Zehong Wu Yameng Hu Ziwen Li Shuxia Zhang Geyan Wu Meisongzhu Yang Miaoling Tang Ruyuan Yu Xinyi Liao Guicheng Gao Wei Zhao Jinkai Wang Jun Li Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma Genomics, Proteomics & Bioinformatics m6A Serine/arginine-rich splicing factor 7 Cell-specific regulation Glioblastoma PDZ-binding kinase |
| title | Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma |
| title_full | Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma |
| title_fullStr | Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma |
| title_full_unstemmed | Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma |
| title_short | Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma |
| title_sort | specific regulation of m6a by srsf7 promotes the progression of glioblastoma |
| topic | m6A Serine/arginine-rich splicing factor 7 Cell-specific regulation Glioblastoma PDZ-binding kinase |
| url | http://www.sciencedirect.com/science/article/pii/S1672022921002527 |
| work_keys_str_mv | AT yixiancun specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT sanqian specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT haiqingzheng specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT jinglan specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT wenfangchen specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT wanjunluo specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT chengguoyao specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT xinchengli specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT xianghuang specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT xiangsun specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT zehongwu specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT yamenghu specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT ziwenli specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT shuxiazhang specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT geyanwu specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT meisongzhuyang specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT miaolingtang specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT ruyuanyu specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT xinyiliao specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT guichenggao specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT weizhao specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT jinkaiwang specificregulationofm6abysrsf7promotestheprogressionofglioblastoma AT junli specificregulationofm6abysrsf7promotestheprogressionofglioblastoma |