Isolation and microencapsulation of Lactobacillus spp. from corn silage for probiotic application

Background and Objectives: Probiotics including strains of Lactobacillus spp. are living microorganisms including which are beneficial to human and animals health. In this study, Lactobacillus has been isolated from corn silage in a cold region of Iran by anaerobic culture. Materials and Methods: Th...

Full description

Bibliographic Details
Main Authors: R Kasra–Kermanshahi, J Fooladi, S Peymanfar
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2010-06-01
Series:Iranian Journal of Microbiology
Subjects:
Online Access:https://ijm.tums.ac.ir/index.php/ijm/article/view/55
_version_ 1818389503683854336
author R Kasra–Kermanshahi
J Fooladi
S Peymanfar
author_facet R Kasra–Kermanshahi
J Fooladi
S Peymanfar
author_sort R Kasra–Kermanshahi
collection DOAJ
description Background and Objectives: Probiotics including strains of Lactobacillus spp. are living microorganisms including which are beneficial to human and animals health. In this study, Lactobacillus has been isolated from corn silage in a cold region of Iran by anaerobic culture. Materials and Methods: The bacteriological and biochemical standard methods were used for identification and phenotypic characterization of isolated organism. To increase the stability of organism in the environment, we used microencapsulation technique using stabilizer polymers (Alginate and Chitosan). Results: The isolated Lactobacillus spp. was able to ferment tested carbohydrates and grow at 10°C -50°C. Using microencapsulation, the stability and survival of this bacterium increased. conclusion: microencapsulation of lactic acid bacteria with alginate and chitosan coating offers an effective way of delivering viable bacterial cells to the colon and maintaining their survival during refrigerated storage.
first_indexed 2024-12-14T04:42:46Z
format Article
id doaj.art-1b965c88b1a846c48a214195096c4067
institution Directory Open Access Journal
issn 2008-3289
2008-4447
language English
last_indexed 2024-12-14T04:42:46Z
publishDate 2010-06-01
publisher Tehran University of Medical Sciences
record_format Article
series Iranian Journal of Microbiology
spelling doaj.art-1b965c88b1a846c48a214195096c40672022-12-21T23:16:45ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472010-06-0122Isolation and microencapsulation of Lactobacillus spp. from corn silage for probiotic applicationR Kasra–Kermanshahi0J Fooladi1S Peymanfar2Department of Biological Science, Alzahra University, Vanak, Tehran, Iran.Department of Biological Science, Alzahra University, Vanak, Tehran, Iran.Department of Biological Science, Alzahra University, Vanak, Tehran, Iran.Background and Objectives: Probiotics including strains of Lactobacillus spp. are living microorganisms including which are beneficial to human and animals health. In this study, Lactobacillus has been isolated from corn silage in a cold region of Iran by anaerobic culture. Materials and Methods: The bacteriological and biochemical standard methods were used for identification and phenotypic characterization of isolated organism. To increase the stability of organism in the environment, we used microencapsulation technique using stabilizer polymers (Alginate and Chitosan). Results: The isolated Lactobacillus spp. was able to ferment tested carbohydrates and grow at 10°C -50°C. Using microencapsulation, the stability and survival of this bacterium increased. conclusion: microencapsulation of lactic acid bacteria with alginate and chitosan coating offers an effective way of delivering viable bacterial cells to the colon and maintaining their survival during refrigerated storage.https://ijm.tums.ac.ir/index.php/ijm/article/view/55Lactobacillussilagmicroencapsulationchitosanalginate
spellingShingle R Kasra–Kermanshahi
J Fooladi
S Peymanfar
Isolation and microencapsulation of Lactobacillus spp. from corn silage for probiotic application
Iranian Journal of Microbiology
Lactobacillus
silag
microencapsulation
chitosan
alginate
title Isolation and microencapsulation of Lactobacillus spp. from corn silage for probiotic application
title_full Isolation and microencapsulation of Lactobacillus spp. from corn silage for probiotic application
title_fullStr Isolation and microencapsulation of Lactobacillus spp. from corn silage for probiotic application
title_full_unstemmed Isolation and microencapsulation of Lactobacillus spp. from corn silage for probiotic application
title_short Isolation and microencapsulation of Lactobacillus spp. from corn silage for probiotic application
title_sort isolation and microencapsulation of lactobacillus spp from corn silage for probiotic application
topic Lactobacillus
silag
microencapsulation
chitosan
alginate
url https://ijm.tums.ac.ir/index.php/ijm/article/view/55
work_keys_str_mv AT rkasrakermanshahi isolationandmicroencapsulationoflactobacillussppfromcornsilageforprobioticapplication
AT jfooladi isolationandmicroencapsulationoflactobacillussppfromcornsilageforprobioticapplication
AT speymanfar isolationandmicroencapsulationoflactobacillussppfromcornsilageforprobioticapplication