Stability of Metabolomic Content during Sample Preparation: Blood and Brain Tissues
Thermal and enzymatic reactions can significantly change the tissue metabolomic content during the sample preparation. In this work, we evaluated the stability of metabolites in human whole blood, serum, and rat brain, as well as in metabolomic extracts from these tissues. We measured the concentrat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/9/811 |
| _version_ | 1797484853595209728 |
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| author | Maxim V. Fomenko Lyudmila V. Yanshole Yuri P. Tsentalovich |
| author_facet | Maxim V. Fomenko Lyudmila V. Yanshole Yuri P. Tsentalovich |
| author_sort | Maxim V. Fomenko |
| collection | DOAJ |
| description | Thermal and enzymatic reactions can significantly change the tissue metabolomic content during the sample preparation. In this work, we evaluated the stability of metabolites in human whole blood, serum, and rat brain, as well as in metabolomic extracts from these tissues. We measured the concentrations of 63 metabolites in brain and 52 metabolites in blood. We have shown that metabolites in the extracts from biological tissues are stable within 24 h at 4 °C. Serum and whole blood metabolomes are also rather stable, changes in metabolomic content of the whole blood homogenate become apparent only after 1–2 h of incubation at 4 °C, and become strong after 24 h. The most significant changes correspond to energy metabolites: the concentrations of ATP and ADP decrease fivefold, and the concentrations of NAD, NADH, and NADPH decrease below the detectable level. A statistically significant increase was observed for AMP, IMP, hypoxanthine, and nicotinamide. The brain tissue is much more metabolically active than human blood, and significant metabolomic changes occur already within the first several minutes during the brain harvest and sample homogenization. At a longer timescale (hours), noticeable changes were observed for all classes of compounds, including amino acids, organic acids, alcohols, amines, sugars, nitrogenous bases, nucleotides, and nucleosides. |
| first_indexed | 2024-03-09T23:11:26Z |
| format | Article |
| id | doaj.art-1b969f5603fb4126b551742cda115fe5 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T23:11:26Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-1b969f5603fb4126b551742cda115fe52023-11-23T17:44:02ZengMDPI AGMetabolites2218-19892022-08-0112981110.3390/metabo12090811Stability of Metabolomic Content during Sample Preparation: Blood and Brain TissuesMaxim V. Fomenko0Lyudmila V. Yanshole1Yuri P. Tsentalovich2Laboratory of Proteomics and Metabolomics, International Tomography Center SB RAS, Institutskaya 3a, 630090 Novosibirsk, RussiaLaboratory of Proteomics and Metabolomics, International Tomography Center SB RAS, Institutskaya 3a, 630090 Novosibirsk, RussiaLaboratory of Proteomics and Metabolomics, International Tomography Center SB RAS, Institutskaya 3a, 630090 Novosibirsk, RussiaThermal and enzymatic reactions can significantly change the tissue metabolomic content during the sample preparation. In this work, we evaluated the stability of metabolites in human whole blood, serum, and rat brain, as well as in metabolomic extracts from these tissues. We measured the concentrations of 63 metabolites in brain and 52 metabolites in blood. We have shown that metabolites in the extracts from biological tissues are stable within 24 h at 4 °C. Serum and whole blood metabolomes are also rather stable, changes in metabolomic content of the whole blood homogenate become apparent only after 1–2 h of incubation at 4 °C, and become strong after 24 h. The most significant changes correspond to energy metabolites: the concentrations of ATP and ADP decrease fivefold, and the concentrations of NAD, NADH, and NADPH decrease below the detectable level. A statistically significant increase was observed for AMP, IMP, hypoxanthine, and nicotinamide. The brain tissue is much more metabolically active than human blood, and significant metabolomic changes occur already within the first several minutes during the brain harvest and sample homogenization. At a longer timescale (hours), noticeable changes were observed for all classes of compounds, including amino acids, organic acids, alcohols, amines, sugars, nitrogenous bases, nucleotides, and nucleosides.https://www.mdpi.com/2218-1989/12/9/811sample preparationhuman bloodrat brainmetabolomicsNMR spectroscopy |
| spellingShingle | Maxim V. Fomenko Lyudmila V. Yanshole Yuri P. Tsentalovich Stability of Metabolomic Content during Sample Preparation: Blood and Brain Tissues Metabolites sample preparation human blood rat brain metabolomics NMR spectroscopy |
| title | Stability of Metabolomic Content during Sample Preparation: Blood and Brain Tissues |
| title_full | Stability of Metabolomic Content during Sample Preparation: Blood and Brain Tissues |
| title_fullStr | Stability of Metabolomic Content during Sample Preparation: Blood and Brain Tissues |
| title_full_unstemmed | Stability of Metabolomic Content during Sample Preparation: Blood and Brain Tissues |
| title_short | Stability of Metabolomic Content during Sample Preparation: Blood and Brain Tissues |
| title_sort | stability of metabolomic content during sample preparation blood and brain tissues |
| topic | sample preparation human blood rat brain metabolomics NMR spectroscopy |
| url | https://www.mdpi.com/2218-1989/12/9/811 |
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