Comparing Protection of Remote Limb with Resveratrol Preconditioning following Rodent Subarachnoid Hemorrhage
Background: Preventing delayed cerebral ischemia (DCI) after subarachnoid hemorrhage (SAH) remains an important therapeutic target. Preconditioning stimulates multiple endogenous protective mechanisms and may be a suitable treatment for DCI following SAH. We here compare remote limb conditioning wit...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/4/568 |
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| author | Sebastian Koch Giselle De La Rua Donnae Farquharson Isabel Saul Miguel Perez-Pinzon Kunjan Dave |
| author_facet | Sebastian Koch Giselle De La Rua Donnae Farquharson Isabel Saul Miguel Perez-Pinzon Kunjan Dave |
| author_sort | Sebastian Koch |
| collection | DOAJ |
| description | Background: Preventing delayed cerebral ischemia (DCI) after subarachnoid hemorrhage (SAH) remains an important therapeutic target. Preconditioning stimulates multiple endogenous protective mechanisms and may be a suitable treatment for DCI following SAH. We here compare remote limb conditioning with resveratrol conditioning in a clinically relevant SAH model. Methods: We produced a SAH in 39 male Sprague Dawley rats using a single injection model. Animals were randomized to four groups: repetitive limb conditioning with a blood pressure cuff, sham conditioning, intraperitoneal resveratrol (10 mg/kg) or intraperitoneal vehicle administered at 24, 48 and 72 h after SAH. On day 4 neurological and behavioral scores were obtained, and animals were euthanized. The cross-sectional area of the basilar artery was measured at the vertebrobasilar junction, and at the mid and distal segments. Hippocampal cells were counted in both hemispheres and normalized per mm length. We compared true limb preconditioning with sham conditioning and resveratrol with vehicle preconditioning. Results: The cross-sectional area of the mid-basilar artery in the true limb preconditioning group was significantly larger by 43% (<i>p</i> = 0.03) when compared with the sham preconditioning group. No differences in the cross-sectional area were found in the resveratrol-treated group when compared to the vehicle-treated group. We found no differences in the neuro score, behavioral score, and in mean hippocampal neuron counts between the groups. Conclusion: We found beneficial vascular effects of remote limb preconditioning on SAH-induced basilar artery vasoconstriction. Our findings support further studies of limb preconditioning as a potential treatment after SAH. |
| first_indexed | 2024-03-09T11:05:51Z |
| format | Article |
| id | doaj.art-1b9a0320c57f4a7d800ae93dede7897c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T11:05:51Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-1b9a0320c57f4a7d800ae93dede7897c2023-12-01T00:57:00ZengMDPI AGBiomolecules2218-273X2022-04-0112456810.3390/biom12040568Comparing Protection of Remote Limb with Resveratrol Preconditioning following Rodent Subarachnoid HemorrhageSebastian Koch0Giselle De La Rua1Donnae Farquharson2Isabel Saul3Miguel Perez-Pinzon4Kunjan Dave5Department of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USADepartment of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USADepartment of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USADepartment of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USADepartment of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USADepartment of Neurology, Miller School of Medicine, University of Miami, Miami, FL 33136, USABackground: Preventing delayed cerebral ischemia (DCI) after subarachnoid hemorrhage (SAH) remains an important therapeutic target. Preconditioning stimulates multiple endogenous protective mechanisms and may be a suitable treatment for DCI following SAH. We here compare remote limb conditioning with resveratrol conditioning in a clinically relevant SAH model. Methods: We produced a SAH in 39 male Sprague Dawley rats using a single injection model. Animals were randomized to four groups: repetitive limb conditioning with a blood pressure cuff, sham conditioning, intraperitoneal resveratrol (10 mg/kg) or intraperitoneal vehicle administered at 24, 48 and 72 h after SAH. On day 4 neurological and behavioral scores were obtained, and animals were euthanized. The cross-sectional area of the basilar artery was measured at the vertebrobasilar junction, and at the mid and distal segments. Hippocampal cells were counted in both hemispheres and normalized per mm length. We compared true limb preconditioning with sham conditioning and resveratrol with vehicle preconditioning. Results: The cross-sectional area of the mid-basilar artery in the true limb preconditioning group was significantly larger by 43% (<i>p</i> = 0.03) when compared with the sham preconditioning group. No differences in the cross-sectional area were found in the resveratrol-treated group when compared to the vehicle-treated group. We found no differences in the neuro score, behavioral score, and in mean hippocampal neuron counts between the groups. Conclusion: We found beneficial vascular effects of remote limb preconditioning on SAH-induced basilar artery vasoconstriction. Our findings support further studies of limb preconditioning as a potential treatment after SAH.https://www.mdpi.com/2218-273X/12/4/568preconditioningsubarachnoid hemorrhageresveratrollimb preconditioning |
| spellingShingle | Sebastian Koch Giselle De La Rua Donnae Farquharson Isabel Saul Miguel Perez-Pinzon Kunjan Dave Comparing Protection of Remote Limb with Resveratrol Preconditioning following Rodent Subarachnoid Hemorrhage Biomolecules preconditioning subarachnoid hemorrhage resveratrol limb preconditioning |
| title | Comparing Protection of Remote Limb with Resveratrol Preconditioning following Rodent Subarachnoid Hemorrhage |
| title_full | Comparing Protection of Remote Limb with Resveratrol Preconditioning following Rodent Subarachnoid Hemorrhage |
| title_fullStr | Comparing Protection of Remote Limb with Resveratrol Preconditioning following Rodent Subarachnoid Hemorrhage |
| title_full_unstemmed | Comparing Protection of Remote Limb with Resveratrol Preconditioning following Rodent Subarachnoid Hemorrhage |
| title_short | Comparing Protection of Remote Limb with Resveratrol Preconditioning following Rodent Subarachnoid Hemorrhage |
| title_sort | comparing protection of remote limb with resveratrol preconditioning following rodent subarachnoid hemorrhage |
| topic | preconditioning subarachnoid hemorrhage resveratrol limb preconditioning |
| url | https://www.mdpi.com/2218-273X/12/4/568 |
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