Tick-borne pathogens in questing adults Dermacentor reticulatus from the Eastern European population (north-eastern Poland)
Abstract Dermacentor reticulatus is tick species with an expanding geographical range in Europe, which creates the possibility of spreading microorganisms of significant veterinary and medical importance. The study aimed to investigate the prevalence and genetic diversity of Rickettsia spp., Babesia...
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-51299-x |
| _version_ | 1797363281620369408 |
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| author | Katarzyna Kubiak Hanna Szymańska Janina Dziekońska-Rynko Agnieszka Tylkowska Małgorzata Dmitryjuk Ewa Dzika |
| author_facet | Katarzyna Kubiak Hanna Szymańska Janina Dziekońska-Rynko Agnieszka Tylkowska Małgorzata Dmitryjuk Ewa Dzika |
| author_sort | Katarzyna Kubiak |
| collection | DOAJ |
| description | Abstract Dermacentor reticulatus is tick species with an expanding geographical range in Europe, which creates the possibility of spreading microorganisms of significant veterinary and medical importance. The study aimed to investigate the prevalence and genetic diversity of Rickettsia spp., Babesia spp., Borrelia spp. and Anaplasma phagocytophilum in adult D. reticulatus ticks from the Eastern European population in the urban and the natural biotopes of north-eastern Poland. Microorganisms were detected by PCR and identified by DNA sequencing. The overall infection rate of at least one of the pathogens was 29.6%. The predominantly was Rickettsia spp. (27.1%) (with R. raoultii—9.1%) followed by Babesia spp. (2.4%) with B. canis (1.5%) as the most frequent. Based on 18S rRNA gene sequence, three B. canis genotypes were revealed. The prevalence of R. raoultii and B. canis was significantly higher in ticks from natural biotopes. The infection rates of B. afzelii and A. phagocytophilum were determined at 0.9% and 0.3%, respectively. Co-infections were detected in 3.8% of infected ticks. In diagnosing tick-borne diseases in humans, tick-borne lymphadenopathy should not be excluded. The prevalence of different genotypes of B. canis suggests differences in the clinical picture of canine babesiosis in the area. |
| first_indexed | 2024-03-08T16:19:07Z |
| format | Article |
| id | doaj.art-1b9c58626c564accba8ec163c8169b76 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T16:19:07Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1b9c58626c564accba8ec163c8169b762024-01-07T12:27:14ZengNature PortfolioScientific Reports2045-23222024-01-0114111010.1038/s41598-024-51299-xTick-borne pathogens in questing adults Dermacentor reticulatus from the Eastern European population (north-eastern Poland)Katarzyna Kubiak0Hanna Szymańska1Janina Dziekońska-Rynko2Agnieszka Tylkowska3Małgorzata Dmitryjuk4Ewa Dzika5Department of Medical Biology, School of Public Health, Collegium Medicum, University of Warmia and Mazury in OlsztynDepartment of Medical Biology, School of Public Health, Collegium Medicum, University of Warmia and Mazury in OlsztynDepartment of Zoology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynDepartment of Biology of Animal Environment, Institute of Animal Science, Warsaw University of Life SciencesDepartment of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury in OlsztynDepartment of Medical Biology, School of Public Health, Collegium Medicum, University of Warmia and Mazury in OlsztynAbstract Dermacentor reticulatus is tick species with an expanding geographical range in Europe, which creates the possibility of spreading microorganisms of significant veterinary and medical importance. The study aimed to investigate the prevalence and genetic diversity of Rickettsia spp., Babesia spp., Borrelia spp. and Anaplasma phagocytophilum in adult D. reticulatus ticks from the Eastern European population in the urban and the natural biotopes of north-eastern Poland. Microorganisms were detected by PCR and identified by DNA sequencing. The overall infection rate of at least one of the pathogens was 29.6%. The predominantly was Rickettsia spp. (27.1%) (with R. raoultii—9.1%) followed by Babesia spp. (2.4%) with B. canis (1.5%) as the most frequent. Based on 18S rRNA gene sequence, three B. canis genotypes were revealed. The prevalence of R. raoultii and B. canis was significantly higher in ticks from natural biotopes. The infection rates of B. afzelii and A. phagocytophilum were determined at 0.9% and 0.3%, respectively. Co-infections were detected in 3.8% of infected ticks. In diagnosing tick-borne diseases in humans, tick-borne lymphadenopathy should not be excluded. The prevalence of different genotypes of B. canis suggests differences in the clinical picture of canine babesiosis in the area.https://doi.org/10.1038/s41598-024-51299-x |
| spellingShingle | Katarzyna Kubiak Hanna Szymańska Janina Dziekońska-Rynko Agnieszka Tylkowska Małgorzata Dmitryjuk Ewa Dzika Tick-borne pathogens in questing adults Dermacentor reticulatus from the Eastern European population (north-eastern Poland) Scientific Reports |
| title | Tick-borne pathogens in questing adults Dermacentor reticulatus from the Eastern European population (north-eastern Poland) |
| title_full | Tick-borne pathogens in questing adults Dermacentor reticulatus from the Eastern European population (north-eastern Poland) |
| title_fullStr | Tick-borne pathogens in questing adults Dermacentor reticulatus from the Eastern European population (north-eastern Poland) |
| title_full_unstemmed | Tick-borne pathogens in questing adults Dermacentor reticulatus from the Eastern European population (north-eastern Poland) |
| title_short | Tick-borne pathogens in questing adults Dermacentor reticulatus from the Eastern European population (north-eastern Poland) |
| title_sort | tick borne pathogens in questing adults dermacentor reticulatus from the eastern european population north eastern poland |
| url | https://doi.org/10.1038/s41598-024-51299-x |
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