High-risk Human papillomavirus 16/18 in oral mucosa and cervix of sexually active women: A comparative pilot study using conventional nested PCR and an in-house real-time PCR
Background: Human papillomavirus (HPV) is known to be associated with both cervical and oropharyngeal cancers, yet little are known about the co-occurrence of similar HPV subtypes in these subsites. With the changing sexual behaviour it is necessary to evaluate the risk of a woman with abnormal cerv...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Journal of Clinical Virology Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667038022000448 |
| _version_ | 1811298191906177024 |
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| author | Pushkal Sinduvadi Ramesh Rahul Vyas Ruthvik Satishchandra Arun Brunda K B Suma Suma M Nataraj Avinash Shetty Veena Shetty Devanand Devegowda |
| author_facet | Pushkal Sinduvadi Ramesh Rahul Vyas Ruthvik Satishchandra Arun Brunda K B Suma Suma M Nataraj Avinash Shetty Veena Shetty Devanand Devegowda |
| author_sort | Pushkal Sinduvadi Ramesh |
| collection | DOAJ |
| description | Background: Human papillomavirus (HPV) is known to be associated with both cervical and oropharyngeal cancers, yet little are known about the co-occurrence of similar HPV subtypes in these subsites. With the changing sexual behaviour it is necessary to evaluate the risk of a woman with abnormal cervix developing cancer of the oropharynx. A study evaluating HPV infection in both oral mucosa and the cervix is therefore needed to understand the co-occurrence of HPV infection in 2 different sites Methods: Oral mucosa and cervical wash specimens were collected from a total of 100 women aged 20-60 years who visited for routine Pap test. DNA was extracted and then subjected to an in house real-time PCR and conventional nested PCR for the detection high risk HPV 16/18 subtypes. A limit of detection was established using serially diluted HPV positive DNA from cell lines and the sensitivity and specificity of both the assays were evaluated Results: The prevalence of HPV-16/18 in the cervical samples (19.7% vs 6.2%) and the oral mucosa (16.6% vs nil) were higher as detected by our in house real-time PCR in comparison to the conventional nested PCR. Oral HPV infection was found in 25% of the women with SCC of the cervix Conclusion: Due to the changing sexual behaviour there is a possibility that women with HPV-related cervical lesions can easily transfer the virus to the partner or even self-inoculate which might lead to the development of lesions in the head & neck turning into cancer. |
| first_indexed | 2024-04-13T06:15:56Z |
| format | Article |
| id | doaj.art-1b9ce32b795a41be8c3dc75657b9b77c |
| institution | Directory Open Access Journal |
| issn | 2667-0380 |
| language | English |
| last_indexed | 2024-04-13T06:15:56Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Clinical Virology Plus |
| spelling | doaj.art-1b9ce32b795a41be8c3dc75657b9b77c2022-12-22T02:58:49ZengElsevierJournal of Clinical Virology Plus2667-03802022-11-0124100105High-risk Human papillomavirus 16/18 in oral mucosa and cervix of sexually active women: A comparative pilot study using conventional nested PCR and an in-house real-time PCRPushkal Sinduvadi Ramesh0Rahul Vyas1Ruthvik Satishchandra2Arun Brunda3K B Suma4Suma M Nataraj5Avinash Shetty6Veena Shetty7Devanand Devegowda8Centre of Excellence in Molecular Biology & Regenerative Medicine (DST-FIST Sponsored), Department of Biochemistry, JSS Medical College, JSS Academy of Higher Education & Research, Mysuru 570015, IndiaJSS Medical College, JSS Academy of Higher Education & Research, Mysuru 570015, IndiaJSS Medical College, JSS Academy of Higher Education & Research, Mysuru 570015, IndiaDepartment of Biochemistry, JSS Medical College, JSS Academy of Higher Education & Research, Mysuru 570015, IndiaDepartment of Obstetrics and Gynecology, JSS Medical College, JSS Academy of Higher Education & Research, Mysuru 570015, IndiaDepartment of Biochemistry, JSS Medical College, JSS Academy of Higher Education & Research, Mysuru 570015, IndiaWake Forest University School of Medicine, Brenner Children's Hospital Winston Salem, NC, USADepartment of Microbiology, K S Hegde Medical Academy, Nitte (Deemed to be University), Deralakatte, Mangalore 575018, IndiaCentre of Excellence in Molecular Biology & Regenerative Medicine (DST-FIST Sponsored), Department of Biochemistry, JSS Medical College, JSS Academy of Higher Education & Research, Mysuru 570015, India; Corresponding author.Background: Human papillomavirus (HPV) is known to be associated with both cervical and oropharyngeal cancers, yet little are known about the co-occurrence of similar HPV subtypes in these subsites. With the changing sexual behaviour it is necessary to evaluate the risk of a woman with abnormal cervix developing cancer of the oropharynx. A study evaluating HPV infection in both oral mucosa and the cervix is therefore needed to understand the co-occurrence of HPV infection in 2 different sites Methods: Oral mucosa and cervical wash specimens were collected from a total of 100 women aged 20-60 years who visited for routine Pap test. DNA was extracted and then subjected to an in house real-time PCR and conventional nested PCR for the detection high risk HPV 16/18 subtypes. A limit of detection was established using serially diluted HPV positive DNA from cell lines and the sensitivity and specificity of both the assays were evaluated Results: The prevalence of HPV-16/18 in the cervical samples (19.7% vs 6.2%) and the oral mucosa (16.6% vs nil) were higher as detected by our in house real-time PCR in comparison to the conventional nested PCR. Oral HPV infection was found in 25% of the women with SCC of the cervix Conclusion: Due to the changing sexual behaviour there is a possibility that women with HPV-related cervical lesions can easily transfer the virus to the partner or even self-inoculate which might lead to the development of lesions in the head & neck turning into cancer.http://www.sciencedirect.com/science/article/pii/S2667038022000448Human papillomavirusNested PCRHigh-risk HPVMolecular diagnosticsCervical cancerOropharyngeal cancer |
| spellingShingle | Pushkal Sinduvadi Ramesh Rahul Vyas Ruthvik Satishchandra Arun Brunda K B Suma Suma M Nataraj Avinash Shetty Veena Shetty Devanand Devegowda High-risk Human papillomavirus 16/18 in oral mucosa and cervix of sexually active women: A comparative pilot study using conventional nested PCR and an in-house real-time PCR Journal of Clinical Virology Plus Human papillomavirus Nested PCR High-risk HPV Molecular diagnostics Cervical cancer Oropharyngeal cancer |
| title | High-risk Human papillomavirus 16/18 in oral mucosa and cervix of sexually active women: A comparative pilot study using conventional nested PCR and an in-house real-time PCR |
| title_full | High-risk Human papillomavirus 16/18 in oral mucosa and cervix of sexually active women: A comparative pilot study using conventional nested PCR and an in-house real-time PCR |
| title_fullStr | High-risk Human papillomavirus 16/18 in oral mucosa and cervix of sexually active women: A comparative pilot study using conventional nested PCR and an in-house real-time PCR |
| title_full_unstemmed | High-risk Human papillomavirus 16/18 in oral mucosa and cervix of sexually active women: A comparative pilot study using conventional nested PCR and an in-house real-time PCR |
| title_short | High-risk Human papillomavirus 16/18 in oral mucosa and cervix of sexually active women: A comparative pilot study using conventional nested PCR and an in-house real-time PCR |
| title_sort | high risk human papillomavirus 16 18 in oral mucosa and cervix of sexually active women a comparative pilot study using conventional nested pcr and an in house real time pcr |
| topic | Human papillomavirus Nested PCR High-risk HPV Molecular diagnostics Cervical cancer Oropharyngeal cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2667038022000448 |
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