Biomolecules resveratrol + coenzyme Q10 recover the cell state of human mesenchymal stem cells after 1-methyl-4-phenylpyridinium-induced damage and improve proliferation and neural differentiation
Neurodegenerative disorders are a critical affection with a high incidence around the world. Currently, there are no effective treatments to solve this problem. However, the application of mesenchymal stem cells (MSCs) and antioxidants in neurodegenerative diseases has shown to be a promising tool d...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.929590/full |
| _version_ | 1817993169381359616 |
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| author | Oscar R. Hernández-Pérez Karen J. Juárez-Navarro Nestor F. Diaz Eduardo Padilla-Camberos Miguel J. Beltran-Garcia Dalila Cardenas-Castrejon Héctor Corona-Perez Claudia Hernández-Jiménez Néstor E. Díaz-Martínez |
| author_facet | Oscar R. Hernández-Pérez Karen J. Juárez-Navarro Nestor F. Diaz Eduardo Padilla-Camberos Miguel J. Beltran-Garcia Dalila Cardenas-Castrejon Héctor Corona-Perez Claudia Hernández-Jiménez Néstor E. Díaz-Martínez |
| author_sort | Oscar R. Hernández-Pérez |
| collection | DOAJ |
| description | Neurodegenerative disorders are a critical affection with a high incidence around the world. Currently, there are no effective treatments to solve this problem. However, the application of mesenchymal stem cells (MSCs) and antioxidants in neurodegenerative diseases has shown to be a promising tool due to their multiple therapeutic effects. This work aimed to evaluate the effects of a combination of resveratrol (RSV) and coenzyme Q10 (CoQ10) on the proliferation and differentiation of MSC and the protector effects in induced damage. To characterize the MSCs, we performed flow cytometry, protocols of cellular differentiation, and immunocytochemistry analysis. The impact of RSV + CoQ10 in proliferation was evaluated by supplementing 2.5 and 10 μM of RSV + CoQ10 in a cellular kinetic for 14 days. Cell viability and lactate dehydrogenase levels (LDH) were also analyzed. The protective effect of RSV + CoQ10 was assessed by supplementing the treatment to damaged MSCs by 1-methyl-4-phenylpyridinium (MPP+); cellular viability, LDH, and reactive oxygen species (ROS) were evaluated.. MSCs expressed the surface markers CD44, CD73, CD90, and CD105 and showed multipotential ability. The combination of RSV + CoQ10 increased the proliferation potential and cell viability and decreased LDH levels. In addition, it reverted the effect of MPP+-induced damage in MSCs to enhance cell viability and decrease LDH and ROS. Finally, RSV + CoQ10 promoted the differentiation of neural progenitors. The combination of RSV + CoQ10 represents a potential treatment to improve MSCs capacities and protect against neurodegenerative damage. |
| first_indexed | 2024-04-14T01:36:24Z |
| format | Article |
| id | doaj.art-1b9d17621b01472cb9afc3e84e0285a5 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-14T01:36:24Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-1b9d17621b01472cb9afc3e84e0285a52022-12-22T02:19:55ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-08-011610.3389/fnins.2022.929590929590Biomolecules resveratrol + coenzyme Q10 recover the cell state of human mesenchymal stem cells after 1-methyl-4-phenylpyridinium-induced damage and improve proliferation and neural differentiationOscar R. Hernández-Pérez0Karen J. Juárez-Navarro1Nestor F. Diaz2Eduardo Padilla-Camberos3Miguel J. Beltran-Garcia4Dalila Cardenas-Castrejon5Héctor Corona-Perez6Claudia Hernández-Jiménez7Néstor E. Díaz-Martínez8Laboratorio de Reprogramación Celular y Bioingeniería de Tejidos, Biotecnología Médica y Farmacéutica, CONACYT Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara, MexicoLaboratorio de Reprogramación Celular y Bioingeniería de Tejidos, Biotecnología Médica y Farmacéutica, CONACYT Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara, MexicoInstituto Nacional de Perinatología (INPER), Mexico City, MexicoBiotecnología Médica y Farmacéutica, CONACYT Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara, MexicoDepartamento de Biotecnológicas y Ambientales, Universidad Autónoma de Guadalajara, Zapopan, MexicoFoundation LCells, Guadalajara, MexicoFoundation LCells, Guadalajara, MexicoCirugía Experimental, Instituto Nacional de Enfermedades Respiratorias (INER), Mexico City, MexicoLaboratorio de Reprogramación Celular y Bioingeniería de Tejidos, Biotecnología Médica y Farmacéutica, CONACYT Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara, MexicoNeurodegenerative disorders are a critical affection with a high incidence around the world. Currently, there are no effective treatments to solve this problem. However, the application of mesenchymal stem cells (MSCs) and antioxidants in neurodegenerative diseases has shown to be a promising tool due to their multiple therapeutic effects. This work aimed to evaluate the effects of a combination of resveratrol (RSV) and coenzyme Q10 (CoQ10) on the proliferation and differentiation of MSC and the protector effects in induced damage. To characterize the MSCs, we performed flow cytometry, protocols of cellular differentiation, and immunocytochemistry analysis. The impact of RSV + CoQ10 in proliferation was evaluated by supplementing 2.5 and 10 μM of RSV + CoQ10 in a cellular kinetic for 14 days. Cell viability and lactate dehydrogenase levels (LDH) were also analyzed. The protective effect of RSV + CoQ10 was assessed by supplementing the treatment to damaged MSCs by 1-methyl-4-phenylpyridinium (MPP+); cellular viability, LDH, and reactive oxygen species (ROS) were evaluated.. MSCs expressed the surface markers CD44, CD73, CD90, and CD105 and showed multipotential ability. The combination of RSV + CoQ10 increased the proliferation potential and cell viability and decreased LDH levels. In addition, it reverted the effect of MPP+-induced damage in MSCs to enhance cell viability and decrease LDH and ROS. Finally, RSV + CoQ10 promoted the differentiation of neural progenitors. The combination of RSV + CoQ10 represents a potential treatment to improve MSCs capacities and protect against neurodegenerative damage.https://www.frontiersin.org/articles/10.3389/fnins.2022.929590/fullmesenchymal stem cellsantioxidantsproliferationresveratrol (3,5,4′-trihydroxy-trans-stilbene)ubiquinone (coenzyme Q10) |
| spellingShingle | Oscar R. Hernández-Pérez Karen J. Juárez-Navarro Nestor F. Diaz Eduardo Padilla-Camberos Miguel J. Beltran-Garcia Dalila Cardenas-Castrejon Héctor Corona-Perez Claudia Hernández-Jiménez Néstor E. Díaz-Martínez Biomolecules resveratrol + coenzyme Q10 recover the cell state of human mesenchymal stem cells after 1-methyl-4-phenylpyridinium-induced damage and improve proliferation and neural differentiation Frontiers in Neuroscience mesenchymal stem cells antioxidants proliferation resveratrol (3,5,4′-trihydroxy-trans-stilbene) ubiquinone (coenzyme Q10) |
| title | Biomolecules resveratrol + coenzyme Q10 recover the cell state of human mesenchymal stem cells after 1-methyl-4-phenylpyridinium-induced damage and improve proliferation and neural differentiation |
| title_full | Biomolecules resveratrol + coenzyme Q10 recover the cell state of human mesenchymal stem cells after 1-methyl-4-phenylpyridinium-induced damage and improve proliferation and neural differentiation |
| title_fullStr | Biomolecules resveratrol + coenzyme Q10 recover the cell state of human mesenchymal stem cells after 1-methyl-4-phenylpyridinium-induced damage and improve proliferation and neural differentiation |
| title_full_unstemmed | Biomolecules resveratrol + coenzyme Q10 recover the cell state of human mesenchymal stem cells after 1-methyl-4-phenylpyridinium-induced damage and improve proliferation and neural differentiation |
| title_short | Biomolecules resveratrol + coenzyme Q10 recover the cell state of human mesenchymal stem cells after 1-methyl-4-phenylpyridinium-induced damage and improve proliferation and neural differentiation |
| title_sort | biomolecules resveratrol coenzyme q10 recover the cell state of human mesenchymal stem cells after 1 methyl 4 phenylpyridinium induced damage and improve proliferation and neural differentiation |
| topic | mesenchymal stem cells antioxidants proliferation resveratrol (3,5,4′-trihydroxy-trans-stilbene) ubiquinone (coenzyme Q10) |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.929590/full |
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