Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep
Abstract: The objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the...
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| Format: | Article |
| Language: | English |
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Embrapa Informação Tecnológica
2019-11-01
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| Series: | Pesquisa Agropecuária Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102810&tlng=en |
| _version_ | 1818744524507185152 |
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| author | Luis Paulo Batista Sousa Junior Ariana Nascimento Meira Hymerson Costa Azevedo Evandro Nevez Muniz Luiz Lehmann Coutinho Gerson Barreto Mourão Victor Breno Pedrosa Luís Fernando Batista Pinto |
| author_facet | Luis Paulo Batista Sousa Junior Ariana Nascimento Meira Hymerson Costa Azevedo Evandro Nevez Muniz Luiz Lehmann Coutinho Gerson Barreto Mourão Victor Breno Pedrosa Luís Fernando Batista Pinto |
| author_sort | Luis Paulo Batista Sousa Junior |
| collection | DOAJ |
| description | Abstract: The objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the Ovis aries genome. Genotype and allele frequencies were estimated, and the Hardy-Weinberg equilibrium was tested. Fragments containing 2,493 bp (MyoD1), 1,836 bp (MyoG), 2,813 bp (MyF5), 1,126 bp (MyF6), and 2,380 bp (MSTN) were obtained, and, in these sequences, 160 variants were identified. These polymorphisms were distributed as follows: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6), and 10 (MSTN). One hundred and four were novel polymorphisms, 45 in MyoD1, 2 in MyoG, 56 in MyF5, and 1 in MSTN. Regarding site, 61 were in intron (27 in MyoD1, 16 in MyoG, 5 in MyF5, 3 in MyF6, and 10 in MSTN), 87 in coding region (22 in MyoD1, 8 in MyoG, 56 in MyF5, and 1 in MyF6), and 12 on 3’UTR (10 in MyoD1 and 2 in MyF5). Therefore, the MyoD family and MSTN genes have several polymorphisms in Santa Inês sheep, which can be useful for association studies. |
| first_indexed | 2024-12-18T02:45:41Z |
| format | Article |
| id | doaj.art-1ba098e197864d91a955cc8f7e42565f |
| institution | Directory Open Access Journal |
| issn | 1678-3921 |
| language | English |
| last_indexed | 2024-12-18T02:45:41Z |
| publishDate | 2019-11-01 |
| publisher | Embrapa Informação Tecnológica |
| record_format | Article |
| series | Pesquisa Agropecuária Brasileira |
| spelling | doaj.art-1ba098e197864d91a955cc8f7e42565f2022-12-21T21:23:34ZengEmbrapa Informação TecnológicaPesquisa Agropecuária Brasileira1678-39212019-11-015410.1590/s1678-3921.pab2019.v54.01132Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheepLuis Paulo Batista Sousa Juniorhttps://orcid.org/0000-0003-0976-9791Ariana Nascimento Meirahttps://orcid.org/0000-0003-2124-8331Hymerson Costa Azevedohttps://orcid.org/0000-0002-0187-9227Evandro Nevez Munizhttps://orcid.org/0000-0003-2806-229XLuiz Lehmann Coutinhohttps://orcid.org/0000-0002-7266-8881Gerson Barreto Mourãohttps://orcid.org/0000-0002-0990-4108Victor Breno Pedrosahttps://orcid.org/0000-0001-8966-2227Luís Fernando Batista Pintohttps://orcid.org/0000-0002-0831-3293Abstract: The objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the Ovis aries genome. Genotype and allele frequencies were estimated, and the Hardy-Weinberg equilibrium was tested. Fragments containing 2,493 bp (MyoD1), 1,836 bp (MyoG), 2,813 bp (MyF5), 1,126 bp (MyF6), and 2,380 bp (MSTN) were obtained, and, in these sequences, 160 variants were identified. These polymorphisms were distributed as follows: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6), and 10 (MSTN). One hundred and four were novel polymorphisms, 45 in MyoD1, 2 in MyoG, 56 in MyF5, and 1 in MSTN. Regarding site, 61 were in intron (27 in MyoD1, 16 in MyoG, 5 in MyF5, 3 in MyF6, and 10 in MSTN), 87 in coding region (22 in MyoD1, 8 in MyoG, 56 in MyF5, and 1 in MyF6), and 12 on 3’UTR (10 in MyoD1 and 2 in MyF5). Therefore, the MyoD family and MSTN genes have several polymorphisms in Santa Inês sheep, which can be useful for association studies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102810&tlng=enOvis ariescandidate genesfrequenciesvariants |
| spellingShingle | Luis Paulo Batista Sousa Junior Ariana Nascimento Meira Hymerson Costa Azevedo Evandro Nevez Muniz Luiz Lehmann Coutinho Gerson Barreto Mourão Victor Breno Pedrosa Luís Fernando Batista Pinto Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep Pesquisa Agropecuária Brasileira Ovis aries candidate genes frequencies variants |
| title | Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep |
| title_full | Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep |
| title_fullStr | Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep |
| title_full_unstemmed | Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep |
| title_short | Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep |
| title_sort | polymorphisms in myod1 myog myf5 myf6 and mstn genes in santa ines sheep |
| topic | Ovis aries candidate genes frequencies variants |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102810&tlng=en |
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