Abdominal Adipose Tissue Associates With Adiponectin and TNFα in Middle-Aged Healthy Men
IntroductionAdipokines are highly active biopeptides involved in glucose metabolism, insulin regulation and the development and progression of obesity and its associated diseases. It includes, among others, adiponectin, visfatin and tumor necrosis factor alpha (TNFα). The sources of adipokines and t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.874977/full |
| _version_ | 1818113215346769920 |
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| author | Hani Zaidi Hani Zaidi Tonje Aksnes Sissel Åkra Heidi B. Eggesbø Heidi B. Eggesbø Rune Byrkjeland Ingebjørg Seljeflot Ingebjørg Seljeflot Trine B. Opstad Trine B. Opstad |
| author_facet | Hani Zaidi Hani Zaidi Tonje Aksnes Sissel Åkra Heidi B. Eggesbø Heidi B. Eggesbø Rune Byrkjeland Ingebjørg Seljeflot Ingebjørg Seljeflot Trine B. Opstad Trine B. Opstad |
| author_sort | Hani Zaidi |
| collection | DOAJ |
| description | IntroductionAdipokines are highly active biopeptides involved in glucose metabolism, insulin regulation and the development and progression of obesity and its associated diseases. It includes, among others, adiponectin, visfatin and tumor necrosis factor alpha (TNFα). The sources of adipokines and their associations with glucometabolic variables are not completely understood.AimIn this cross-sectional study, we aimed to investigate whether gene expression levels in subcutaneous adipose tissue (SAT) of selected adipokines and their corresponding circulating levels associate with the amount of AT in superficial (sSAT), deep (dSAT) and visceral AT (VAT), assessed by computed tomography (CT). Any association with glucometabolic variables were also explored.MethodsIn 103 healthy Caucasian men, aged 39.5 years, fasting venous blood and SAT samples from the gluteal region were collected. Ninety-four of the participants underwent CT assessment of the abdominal AT, which was divided into VAT, sSAT and dSAT. Circulating levels of adipokines were measured by ELISA and AT gene-expression by PCR. Insulin sensitivity was determined by glucose clamp, assessing glucose disposal rate (GDR).ResultsCirculating adiponectin and TNFα gene expression correlated inversely and positively to the amount of AT in all three compartments (r=-0.266 to -0.276, p<0.05 for all) and (r=0.323 - 0.368, p<0.05 for all), respectively, with strongest correlations to the amount in sSAT and dSAT. When dividing AT compartments into quartiles, a tendency was observed towards lower circulating adiponectin and higher TNFα gene expression levels, respectively, with increasing amount of sSAT and dSAT. Circulating adiponectin correlated inversely to insulin, C-peptide and waist circumference (r=-456 to -0.373, p<0.001) and positively to GDR (r=0.356, p<0.001). AT-expressed visfatin correlated inversely to insulin and C-peptide (r=-0.370 and r=-0.404, p<0.001).ConclusionIncreased amount of AT is associated with lower levels of adiponectin and increased levels of TNFα AT expression. |
| first_indexed | 2024-12-11T03:31:17Z |
| format | Article |
| id | doaj.art-1ba1c38812b2426cb1b0735d69827c13 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-11T03:31:17Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-1ba1c38812b2426cb1b0735d69827c132022-12-22T01:22:22ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-07-011310.3389/fendo.2022.874977874977Abdominal Adipose Tissue Associates With Adiponectin and TNFα in Middle-Aged Healthy MenHani Zaidi0Hani Zaidi1Tonje Aksnes2Sissel Åkra3Heidi B. Eggesbø4Heidi B. Eggesbø5Rune Byrkjeland6Ingebjørg Seljeflot7Ingebjørg Seljeflot8Trine B. Opstad9Trine B. Opstad10Center for Clinical Heart Research, Department of Cardiology, Oslo University Hospital, Ullevål, NorwayFaculty of Medicine, University of Oslo, Oslo, NorwaySection for Interventional Cardiology, Department of Cardiology, Heart-, Lung-, and Vascular-Disease Clinic, Oslo University Hospital, Oslo, NorwayCenter for Clinical Heart Research, Department of Cardiology, Oslo University Hospital, Ullevål, NorwayFaculty of Medicine, University of Oslo, Oslo, NorwayDivision of Radiology and Nuclear Medicine, Oslo University Hospital, Oslo, NorwayCenter for Clinical Heart Research, Department of Cardiology, Oslo University Hospital, Ullevål, NorwayCenter for Clinical Heart Research, Department of Cardiology, Oslo University Hospital, Ullevål, NorwayFaculty of Medicine, University of Oslo, Oslo, NorwayCenter for Clinical Heart Research, Department of Cardiology, Oslo University Hospital, Ullevål, NorwayFaculty of Medicine, University of Oslo, Oslo, NorwayIntroductionAdipokines are highly active biopeptides involved in glucose metabolism, insulin regulation and the development and progression of obesity and its associated diseases. It includes, among others, adiponectin, visfatin and tumor necrosis factor alpha (TNFα). The sources of adipokines and their associations with glucometabolic variables are not completely understood.AimIn this cross-sectional study, we aimed to investigate whether gene expression levels in subcutaneous adipose tissue (SAT) of selected adipokines and their corresponding circulating levels associate with the amount of AT in superficial (sSAT), deep (dSAT) and visceral AT (VAT), assessed by computed tomography (CT). Any association with glucometabolic variables were also explored.MethodsIn 103 healthy Caucasian men, aged 39.5 years, fasting venous blood and SAT samples from the gluteal region were collected. Ninety-four of the participants underwent CT assessment of the abdominal AT, which was divided into VAT, sSAT and dSAT. Circulating levels of adipokines were measured by ELISA and AT gene-expression by PCR. Insulin sensitivity was determined by glucose clamp, assessing glucose disposal rate (GDR).ResultsCirculating adiponectin and TNFα gene expression correlated inversely and positively to the amount of AT in all three compartments (r=-0.266 to -0.276, p<0.05 for all) and (r=0.323 - 0.368, p<0.05 for all), respectively, with strongest correlations to the amount in sSAT and dSAT. When dividing AT compartments into quartiles, a tendency was observed towards lower circulating adiponectin and higher TNFα gene expression levels, respectively, with increasing amount of sSAT and dSAT. Circulating adiponectin correlated inversely to insulin, C-peptide and waist circumference (r=-456 to -0.373, p<0.001) and positively to GDR (r=0.356, p<0.001). AT-expressed visfatin correlated inversely to insulin and C-peptide (r=-0.370 and r=-0.404, p<0.001).ConclusionIncreased amount of AT is associated with lower levels of adiponectin and increased levels of TNFα AT expression.https://www.frontiersin.org/articles/10.3389/fendo.2022.874977/fulladiponectinTNFαadipose tissuegene expressionadipose tissue compartments |
| spellingShingle | Hani Zaidi Hani Zaidi Tonje Aksnes Sissel Åkra Heidi B. Eggesbø Heidi B. Eggesbø Rune Byrkjeland Ingebjørg Seljeflot Ingebjørg Seljeflot Trine B. Opstad Trine B. Opstad Abdominal Adipose Tissue Associates With Adiponectin and TNFα in Middle-Aged Healthy Men Frontiers in Endocrinology adiponectin TNFα adipose tissue gene expression adipose tissue compartments |
| title | Abdominal Adipose Tissue Associates With Adiponectin and TNFα in Middle-Aged Healthy Men |
| title_full | Abdominal Adipose Tissue Associates With Adiponectin and TNFα in Middle-Aged Healthy Men |
| title_fullStr | Abdominal Adipose Tissue Associates With Adiponectin and TNFα in Middle-Aged Healthy Men |
| title_full_unstemmed | Abdominal Adipose Tissue Associates With Adiponectin and TNFα in Middle-Aged Healthy Men |
| title_short | Abdominal Adipose Tissue Associates With Adiponectin and TNFα in Middle-Aged Healthy Men |
| title_sort | abdominal adipose tissue associates with adiponectin and tnfα in middle aged healthy men |
| topic | adiponectin TNFα adipose tissue gene expression adipose tissue compartments |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.874977/full |
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