Rhamnogalacturonan Endolyase Family 4 Enzymes: An Update on Their Importance in the Fruit Ripening Process
Fruit ripening is a process that produces fruit with top sensory qualities that are ideal for consumption. For the plant, the final objective is seed dispersal. One of the fruit characteristics observed by consumers is texture, which is related to the ripening and softening of the fruit. Controlled...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/5/465 |
| _version_ | 1797499344369221632 |
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| author | Luis Morales-Quintana Patricio Ramos Angela Méndez-Yáñez |
| author_facet | Luis Morales-Quintana Patricio Ramos Angela Méndez-Yáñez |
| author_sort | Luis Morales-Quintana |
| collection | DOAJ |
| description | Fruit ripening is a process that produces fruit with top sensory qualities that are ideal for consumption. For the plant, the final objective is seed dispersal. One of the fruit characteristics observed by consumers is texture, which is related to the ripening and softening of the fruit. Controlled and orchestrated events occur to regulate the expression of genes involved in disassembling and solubilizing the cell wall. Studies have shown that changes in pectins are closely related to the loss of firmness and fruit softening. For this reason, studying the mechanisms and enzymes that act on pectins could help to elucidate the molecular events that occur in the fruit. This paper provides a review of the enzyme rhamnogalacturonan endolyase (RGL; EC 4.2.2.23), which is responsible for cleavage of the pectin rhamnogalacturonan I (RGL-I) between rhamnose (Rha) and galacturonic acid (GalA) through the mechanism of β-elimination during fruit ripening. RGL promotes the loosening and weakening of the cell wall and exposes the backbone of the polysaccharide to the action of other enzymes. Investigations into RGL and its relationship with fruit ripening have reliably demonstrated that this enzyme has an important role in this process. |
| first_indexed | 2024-03-10T03:46:04Z |
| format | Article |
| id | doaj.art-1ba23acc347948308632a0aded2973a9 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T03:46:04Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-1ba23acc347948308632a0aded2973a92023-11-23T11:17:45ZengMDPI AGHorticulturae2311-75242022-05-018546510.3390/horticulturae8050465Rhamnogalacturonan Endolyase Family 4 Enzymes: An Update on Their Importance in the Fruit Ripening ProcessLuis Morales-Quintana0Patricio Ramos1Angela Méndez-Yáñez2Multidisciplinary Agroindustry Research Laboratory, Instituto de Ciencias Biomédicas, Universidad Autónoma de Chile, Talca 3466706, ChileCentro de Investigación de Estudios Avanzados del Maule (CIEAM), Vicerrectoría de Investigación y Postgrado, Universidad Católica del Maule, Talca 3466706, ChileMultidisciplinary Agroindustry Research Laboratory, Instituto de Ciencias Biomédicas, Universidad Autónoma de Chile, Talca 3466706, ChileFruit ripening is a process that produces fruit with top sensory qualities that are ideal for consumption. For the plant, the final objective is seed dispersal. One of the fruit characteristics observed by consumers is texture, which is related to the ripening and softening of the fruit. Controlled and orchestrated events occur to regulate the expression of genes involved in disassembling and solubilizing the cell wall. Studies have shown that changes in pectins are closely related to the loss of firmness and fruit softening. For this reason, studying the mechanisms and enzymes that act on pectins could help to elucidate the molecular events that occur in the fruit. This paper provides a review of the enzyme rhamnogalacturonan endolyase (RGL; EC 4.2.2.23), which is responsible for cleavage of the pectin rhamnogalacturonan I (RGL-I) between rhamnose (Rha) and galacturonic acid (GalA) through the mechanism of β-elimination during fruit ripening. RGL promotes the loosening and weakening of the cell wall and exposes the backbone of the polysaccharide to the action of other enzymes. Investigations into RGL and its relationship with fruit ripening have reliably demonstrated that this enzyme has an important role in this process.https://www.mdpi.com/2311-7524/8/5/465fruit ripeningrhamnogalacturonan-Irhamnogalacturonan lyase enzymecell wall remodeling |
| spellingShingle | Luis Morales-Quintana Patricio Ramos Angela Méndez-Yáñez Rhamnogalacturonan Endolyase Family 4 Enzymes: An Update on Their Importance in the Fruit Ripening Process Horticulturae fruit ripening rhamnogalacturonan-I rhamnogalacturonan lyase enzyme cell wall remodeling |
| title | Rhamnogalacturonan Endolyase Family 4 Enzymes: An Update on Their Importance in the Fruit Ripening Process |
| title_full | Rhamnogalacturonan Endolyase Family 4 Enzymes: An Update on Their Importance in the Fruit Ripening Process |
| title_fullStr | Rhamnogalacturonan Endolyase Family 4 Enzymes: An Update on Their Importance in the Fruit Ripening Process |
| title_full_unstemmed | Rhamnogalacturonan Endolyase Family 4 Enzymes: An Update on Their Importance in the Fruit Ripening Process |
| title_short | Rhamnogalacturonan Endolyase Family 4 Enzymes: An Update on Their Importance in the Fruit Ripening Process |
| title_sort | rhamnogalacturonan endolyase family 4 enzymes an update on their importance in the fruit ripening process |
| topic | fruit ripening rhamnogalacturonan-I rhamnogalacturonan lyase enzyme cell wall remodeling |
| url | https://www.mdpi.com/2311-7524/8/5/465 |
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