Evolutionary aspects of the Viridiplantae nitroreductases
Abstract Background Nitroreductases are a family of evolutionarily related proteins catalyzing the reduction of nitro-substituted compounds. Nitroreductases are widespread enzymes, but nearly all modern research and practical application have been concentrated on the bacterial proteins, mainly nitro...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | http://link.springer.com/article/10.1186/s43141-020-00073-3 |
| _version_ | 1797203483238072320 |
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| author | Siarhei A. Dabravolski |
| author_facet | Siarhei A. Dabravolski |
| author_sort | Siarhei A. Dabravolski |
| collection | DOAJ |
| description | Abstract Background Nitroreductases are a family of evolutionarily related proteins catalyzing the reduction of nitro-substituted compounds. Nitroreductases are widespread enzymes, but nearly all modern research and practical application have been concentrated on the bacterial proteins, mainly nitroreductases of Escherichia coli. The main aim of this study is to describe the phylogenic distribution of the nitroreductases in the photosynthetic eukaryotes (Viridiplantae) to highlight their structural similarity and areas for future research and application. Results This study suggests that homologs of nitroreductase proteins are widely presented also in Viridiplantae. Maximum likelihood phylogenetic tree reconstruction method and comparison of the structural models suggest close evolutional relation between cyanobacterial and Viridiplantae nitroreductases. Conclusions This study provides the first attempt to understand the evolution of nitroreductase protein family in Viridiplantae. Our phylogeny estimation and preservation of the chloroplasts/mitochondrial localization indicate the evolutional origin of the plant nitroreductases from the cyanobacterial endosymbiont. A defined high level of the similarity on the structural level suggests conservancy also for the functions. Directions for the future research and industrial application of the Viridiplantae nitroreductases are discussed. |
| first_indexed | 2024-04-24T08:20:03Z |
| format | Article |
| id | doaj.art-1ba4eabf17444b539b867d51b257b2cc |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:20:03Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-1ba4eabf17444b539b867d51b257b2cc2024-04-17T01:25:26ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202020-10-011811710.1186/s43141-020-00073-3Evolutionary aspects of the Viridiplantae nitroreductasesSiarhei A. Dabravolski0Department of Clinical Diagnostics, Vitebsk State Academy of Veterinary Medicine [UO VGAVM]Abstract Background Nitroreductases are a family of evolutionarily related proteins catalyzing the reduction of nitro-substituted compounds. Nitroreductases are widespread enzymes, but nearly all modern research and practical application have been concentrated on the bacterial proteins, mainly nitroreductases of Escherichia coli. The main aim of this study is to describe the phylogenic distribution of the nitroreductases in the photosynthetic eukaryotes (Viridiplantae) to highlight their structural similarity and areas for future research and application. Results This study suggests that homologs of nitroreductase proteins are widely presented also in Viridiplantae. Maximum likelihood phylogenetic tree reconstruction method and comparison of the structural models suggest close evolutional relation between cyanobacterial and Viridiplantae nitroreductases. Conclusions This study provides the first attempt to understand the evolution of nitroreductase protein family in Viridiplantae. Our phylogeny estimation and preservation of the chloroplasts/mitochondrial localization indicate the evolutional origin of the plant nitroreductases from the cyanobacterial endosymbiont. A defined high level of the similarity on the structural level suggests conservancy also for the functions. Directions for the future research and industrial application of the Viridiplantae nitroreductases are discussed.http://link.springer.com/article/10.1186/s43141-020-00073-3NitroreductaseViridiplantaeCyanobacteriaPhylogenyEvolution |
| spellingShingle | Siarhei A. Dabravolski Evolutionary aspects of the Viridiplantae nitroreductases Journal of Genetic Engineering and Biotechnology Nitroreductase Viridiplantae Cyanobacteria Phylogeny Evolution |
| title | Evolutionary aspects of the Viridiplantae nitroreductases |
| title_full | Evolutionary aspects of the Viridiplantae nitroreductases |
| title_fullStr | Evolutionary aspects of the Viridiplantae nitroreductases |
| title_full_unstemmed | Evolutionary aspects of the Viridiplantae nitroreductases |
| title_short | Evolutionary aspects of the Viridiplantae nitroreductases |
| title_sort | evolutionary aspects of the viridiplantae nitroreductases |
| topic | Nitroreductase Viridiplantae Cyanobacteria Phylogeny Evolution |
| url | http://link.springer.com/article/10.1186/s43141-020-00073-3 |
| work_keys_str_mv | AT siarheiadabravolski evolutionaryaspectsoftheviridiplantaenitroreductases |