HPLC Fingerprint Analysis Combined with Chemometrics for Authentication of <i>Kaempferia galanga</i> from Related Species
Fingerprint analysis using high performance liquid chromatography (HPLC) has been developed for authentication of Kaempferia galanga from related species, such as Kaempferia pandurata and K. rotunda. By comparing the fingerprint chromatograms of K. galanga, K. pandurata and K. rotunda, we could iden...
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| Format: | Article |
| Language: | English |
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Department of Chemistry, Universitas Gadjah Mada
2016-12-01
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| Series: | Indonesian Journal of Chemistry |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijc/article/view/21146 |
| _version_ | 1818999368862138368 |
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| author | Cahya Septyanti Irmanida Batubara Mohamad Rafi |
| author_facet | Cahya Septyanti Irmanida Batubara Mohamad Rafi |
| author_sort | Cahya Septyanti |
| collection | DOAJ |
| description | Fingerprint analysis using high performance liquid chromatography (HPLC) has been developed for authentication of Kaempferia galanga from related species, such as Kaempferia pandurata and K. rotunda. By comparing the fingerprint chromatograms of K. galanga, K. pandurata and K. rotunda, we could identify K. galanga samples and detect adulteration of K. galanga from K. pandurata and K. rotunda by using their marker peaks. We also combined HPLC fingerprint with chemometrics for discrimination the three species and also for authentication of K. galanga. All the three species and K. galanga adulterated with K. pandurata and K. rotunda were discriminated successfully by using principal component analysis (PCA) and discriminant analysis (DA). This result indicates that HPLC fingerprint analysis in combination with PCA (PC1 = 30.06% and PC2 = 34.74%) and DA (DF1 = 94.59% and DF2 = 3.32%) could be used for authentication of K. galanga samples from the related species. |
| first_indexed | 2024-12-20T22:16:19Z |
| format | Article |
| id | doaj.art-1ba57c4b1bba491a811106d5568922ac |
| institution | Directory Open Access Journal |
| issn | 1411-9420 2460-1578 |
| language | English |
| last_indexed | 2024-12-20T22:16:19Z |
| publishDate | 2016-12-01 |
| publisher | Department of Chemistry, Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Chemistry |
| spelling | doaj.art-1ba57c4b1bba491a811106d5568922ac2022-12-21T19:25:02ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782016-12-0116330230710.22146/ijc.2114614244HPLC Fingerprint Analysis Combined with Chemometrics for Authentication of <i>Kaempferia galanga</i> from Related SpeciesCahya Septyanti0Irmanida Batubara1Mohamad Rafi2Department of Chemistry, Faculty of Mathematic and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Kimia Building, Tanjung Street, Bogor 16680Department of Chemistry, Faculty of Mathematic and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Kimia Building, Tanjung Street, Bogor 16680 Biopharmaca Research Center-Research and Community Empowerment Institute, Bogor Agricultural University, Bogor 16128Biopharmaca Research Center-Research and Community Empowerment Institute, Bogor Agricultural University, Bogor 16128Fingerprint analysis using high performance liquid chromatography (HPLC) has been developed for authentication of Kaempferia galanga from related species, such as Kaempferia pandurata and K. rotunda. By comparing the fingerprint chromatograms of K. galanga, K. pandurata and K. rotunda, we could identify K. galanga samples and detect adulteration of K. galanga from K. pandurata and K. rotunda by using their marker peaks. We also combined HPLC fingerprint with chemometrics for discrimination the three species and also for authentication of K. galanga. All the three species and K. galanga adulterated with K. pandurata and K. rotunda were discriminated successfully by using principal component analysis (PCA) and discriminant analysis (DA). This result indicates that HPLC fingerprint analysis in combination with PCA (PC1 = 30.06% and PC2 = 34.74%) and DA (DF1 = 94.59% and DF2 = 3.32%) could be used for authentication of K. galanga samples from the related species.https://jurnal.ugm.ac.id/ijc/article/view/21146Kaempferia galangaHPLCFingerprint analysisChemometrics |
| spellingShingle | Cahya Septyanti Irmanida Batubara Mohamad Rafi HPLC Fingerprint Analysis Combined with Chemometrics for Authentication of <i>Kaempferia galanga</i> from Related Species Indonesian Journal of Chemistry Kaempferia galanga HPLC Fingerprint analysis Chemometrics |
| title | HPLC Fingerprint Analysis Combined with Chemometrics for Authentication of <i>Kaempferia galanga</i> from Related Species |
| title_full | HPLC Fingerprint Analysis Combined with Chemometrics for Authentication of <i>Kaempferia galanga</i> from Related Species |
| title_fullStr | HPLC Fingerprint Analysis Combined with Chemometrics for Authentication of <i>Kaempferia galanga</i> from Related Species |
| title_full_unstemmed | HPLC Fingerprint Analysis Combined with Chemometrics for Authentication of <i>Kaempferia galanga</i> from Related Species |
| title_short | HPLC Fingerprint Analysis Combined with Chemometrics for Authentication of <i>Kaempferia galanga</i> from Related Species |
| title_sort | hplc fingerprint analysis combined with chemometrics for authentication of i kaempferia galanga i from related species |
| topic | Kaempferia galanga HPLC Fingerprint analysis Chemometrics |
| url | https://jurnal.ugm.ac.id/ijc/article/view/21146 |
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