Estimation of zeta-chain-associated protein 70, interleukin-6 and interleukin-10 levels in sera of Iraqi newly diagnosed chronic lymphocytic leukemia
Background: Chronic lymphocytic leukemia (CLL) is the most common type of leukemia in the Western world. The zeta-chain-associated protein (ZAP-70) an intracellular tyrosine kinase which play an important role in T-cell receptor signaling, natural killer cell activation, and early B-cell development...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Iraqi Journal of Hematology |
| Subjects: | |
| Online Access: | http://www.ijhonline.org/article.asp?issn=2072-8069;year=2016;volume=5;issue=2;spage=173;epage=177;aulast=Al-Dahery |
| _version_ | 1819059634512592896 |
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| author | Haider S Al-Dahery Alaa Fadhil Alwan Hassnien S Muslit |
| author_facet | Haider S Al-Dahery Alaa Fadhil Alwan Hassnien S Muslit |
| author_sort | Haider S Al-Dahery |
| collection | DOAJ |
| description | Background: Chronic lymphocytic leukemia (CLL) is the most common type of leukemia in the Western world. The zeta-chain-associated protein (ZAP-70) an intracellular tyrosine kinase which play an important role in T-cell receptor signaling, natural killer cell activation, and early B-cell development. Interleukin-10 (IL-10) production has strong immunosuppressive effects through inhibition of Th1 type cytokines. IL-6 is a pleiotropic cytokine produced by a variety of cell types, including normal hematopoietic cells, and lymphocytes.
Objectives: The objective of this study is to assess the level of ZAP-70, IL-10, and IL-6 in CLL and to correlate these levels with prognosis.
Materials and Methods: A prospective cohort study carried out at the National Center of Hematology from October 2013 to September 2015. Eighteen patients with newly diagnosis of CLL compared to 19 apparently healthy controls were also involved in this study. ZAP-70 was measured by while IL-10 and IL-6 were measured using serological methods including the enzyme-linked immunosorbent assay.
Results: ZAP-70 range between (24% and 90%) with a mean of 46.89 ± 19.15, two patients out of 18 were negative (<24%) for ZAP-70 as compared with control. The levels of IL-6 in the serum of untreated patients with CLL were increased in compared with healthy control (2.53 ± 1.98), ranged between (0.12 and 6.94) pg/ml. The range of the IL-10 expression of the untreated CLL patients was between (the lowest positive value (212 pg/ml) and which is the highest positive value (987 pg/ml) with a mean 614 ± 301 pg/ml, with all the morphologically diagnosed CLL cases show positive expression for IL-10.
Conclusion: ZAP-70 level was higher in CLL patients than control and immunochemotherapy can normalize this which indicated good response to treatment. On the other hand, IL6 and IL10 were also higher in patients with CLL but not affected by therapy. |
| first_indexed | 2024-12-21T14:14:13Z |
| format | Article |
| id | doaj.art-1ba81b67a8ef4d679442e5038b11b600 |
| institution | Directory Open Access Journal |
| issn | 2072-8069 |
| language | English |
| last_indexed | 2024-12-21T14:14:13Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Iraqi Journal of Hematology |
| spelling | doaj.art-1ba81b67a8ef4d679442e5038b11b6002022-12-21T19:00:57ZengWolters Kluwer Medknow PublicationsIraqi Journal of Hematology2072-80692016-01-015217317710.4103/2072-8069.198123Estimation of zeta-chain-associated protein 70, interleukin-6 and interleukin-10 levels in sera of Iraqi newly diagnosed chronic lymphocytic leukemiaHaider S Al-DaheryAlaa Fadhil AlwanHassnien S MuslitBackground: Chronic lymphocytic leukemia (CLL) is the most common type of leukemia in the Western world. The zeta-chain-associated protein (ZAP-70) an intracellular tyrosine kinase which play an important role in T-cell receptor signaling, natural killer cell activation, and early B-cell development. Interleukin-10 (IL-10) production has strong immunosuppressive effects through inhibition of Th1 type cytokines. IL-6 is a pleiotropic cytokine produced by a variety of cell types, including normal hematopoietic cells, and lymphocytes. Objectives: The objective of this study is to assess the level of ZAP-70, IL-10, and IL-6 in CLL and to correlate these levels with prognosis. Materials and Methods: A prospective cohort study carried out at the National Center of Hematology from October 2013 to September 2015. Eighteen patients with newly diagnosis of CLL compared to 19 apparently healthy controls were also involved in this study. ZAP-70 was measured by while IL-10 and IL-6 were measured using serological methods including the enzyme-linked immunosorbent assay. Results: ZAP-70 range between (24% and 90%) with a mean of 46.89 ± 19.15, two patients out of 18 were negative (<24%) for ZAP-70 as compared with control. The levels of IL-6 in the serum of untreated patients with CLL were increased in compared with healthy control (2.53 ± 1.98), ranged between (0.12 and 6.94) pg/ml. The range of the IL-10 expression of the untreated CLL patients was between (the lowest positive value (212 pg/ml) and which is the highest positive value (987 pg/ml) with a mean 614 ± 301 pg/ml, with all the morphologically diagnosed CLL cases show positive expression for IL-10. Conclusion: ZAP-70 level was higher in CLL patients than control and immunochemotherapy can normalize this which indicated good response to treatment. On the other hand, IL6 and IL10 were also higher in patients with CLL but not affected by therapy.http://www.ijhonline.org/article.asp?issn=2072-8069;year=2016;volume=5;issue=2;spage=173;epage=177;aulast=Al-DaheryChronic lymphocytic leukemiainterleukin-10interleukin-6zeta-chain-associated protein 70 |
| spellingShingle | Haider S Al-Dahery Alaa Fadhil Alwan Hassnien S Muslit Estimation of zeta-chain-associated protein 70, interleukin-6 and interleukin-10 levels in sera of Iraqi newly diagnosed chronic lymphocytic leukemia Iraqi Journal of Hematology Chronic lymphocytic leukemia interleukin-10 interleukin-6 zeta-chain-associated protein 70 |
| title | Estimation of zeta-chain-associated protein 70, interleukin-6 and interleukin-10 levels in sera of Iraqi newly diagnosed chronic lymphocytic leukemia |
| title_full | Estimation of zeta-chain-associated protein 70, interleukin-6 and interleukin-10 levels in sera of Iraqi newly diagnosed chronic lymphocytic leukemia |
| title_fullStr | Estimation of zeta-chain-associated protein 70, interleukin-6 and interleukin-10 levels in sera of Iraqi newly diagnosed chronic lymphocytic leukemia |
| title_full_unstemmed | Estimation of zeta-chain-associated protein 70, interleukin-6 and interleukin-10 levels in sera of Iraqi newly diagnosed chronic lymphocytic leukemia |
| title_short | Estimation of zeta-chain-associated protein 70, interleukin-6 and interleukin-10 levels in sera of Iraqi newly diagnosed chronic lymphocytic leukemia |
| title_sort | estimation of zeta chain associated protein 70 interleukin 6 and interleukin 10 levels in sera of iraqi newly diagnosed chronic lymphocytic leukemia |
| topic | Chronic lymphocytic leukemia interleukin-10 interleukin-6 zeta-chain-associated protein 70 |
| url | http://www.ijhonline.org/article.asp?issn=2072-8069;year=2016;volume=5;issue=2;spage=173;epage=177;aulast=Al-Dahery |
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